Sıçanlarda Valproik Asite Bağlı Testis Hasarına Rutin Etkisinin Araştırılması

Öz

Valproik asit (VALP), epilepsi gibi birçok psikiyatrik hastalık için kullanılan bir ilaçtır. Ancak VALP kullanımının testisler de dahil olmak üzere çeşitli dokularda potansiyel yan etkileri vardır. Rutin (RUT), oksidatif stres kaynaklı hastalıklara ve lipid peroksidasyonuna karşı koruyucu etkileri olan bir flavonoiddir. Bu çalışmada VALP'in neden olduğu testis hasarına karşı RUT'un koruyucu etkisi araştırıldı. Bu amaçla çalışmada ağırlıkları 220-250 g olan 35 adet erkek Sprague-Dawley cinsi rat kullanıldı. Ratlar kontrol (serum fizyolojik), RUT (100 mg/kg/va), VALP, (500mg/kg/va), VALP+RUT 50 (500 mg/kg/va VALP+50 mg/kg/va RUT) ve VALP +RUT 100 (500 mg/kg/v VALP+100 mg/kg/va RUT) olarak 5 gruba ayrıldı. Uygulamalarının sonunda ratlar sakrifiye edilerek testis dokuları biyokimyasal ve spermatolojik analizlerde kullanılmak üzere alındı. Sunulan çalışmanın sonuçlarına göre VALP grubunun testis dokularındaki MDA düzeyi diğer deney gruplarına göre istatistiksel olarak yüksek bulundu (p<0,05). Testis dokusu GSH ve Nrf-2 düzeyi VALP grubunda en düşüktü. Testis dokusunda TNF-α, IL-1β ve MAPK14 seviyeleri VALP grubunda en yüksek iken, RUT tedavi gruplarında azaldı. Benzer şekilde en yüksek Bax, Caspase-3 ve MMP-9 seviyeleri VALP grubunda gözlenirken, RUT tedavisi bu değeri azaltmıştır. Spermatolojik analizler incelendiğinde total motilite değerinin VALP grubunda anlamlı olarak düştüğü, ancak RUT tedavi grubunda total motilite değerinin arttığı görüldü (p<0,05). VALP grubunda ölü ve anormal sperm oranında önemli bir artış bulundu, ancak bu parametreler RUT tedavi grubunda düzeldi. Sonuç olarak RUT, sıçanlarda VALP'nin neden olduğu testis hasarına karşı koruyucu etkilere sahiptir.

Anahtar Kelimeler

• Oksidatif stres
• rat
• rutin
• sperma
• valproik asit.

Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats

Abstract

Valproic acid (VALP) is a drug used for many psychiatric diseases such as epilepsy. However, the use of VALP has potential side effects on various tissues, including the testicles. Rutin (RUT) is a flavonoid with protective effects against oxidative stress-induced diseases and lipid peroxidation. In this study, the protective effects of RUT against testicular damage caused by VALP were investigated. For this purpose, 35 male Spraque-Dawley rats weighing 220-250 g were used in the study. The rats were randomly divided into 5 groups as Control (physiological saline), RUT (100 mg/kg/bw), VALP, (500mg/kg/bw), VALP+RUT 50 (500 mg/kg/bw VALP+50 mg/kg/bw RUT), and VALP +RUT 100 (500 mg/kg/bw VALP+100 mg/kg/bw RUT). At the end of the RUT and VALP administrations, the rats were sacrificed and testicular tissues were taken to be used for biochemical and spermatological analyzes. According to the results of this study, the MDA level in the testicular tissues of the VALP group was found to be statistically higher than the other experimental groups (p<0.05). Testicular tissue GSH and Nrf-2 level was the lowest in the VALP group. TNF-α, IL-1Β and MAPK14 levels in testicular tissue were highest in the VALP group, while it was decreased in the RUT treatment groups. Similarly, the highest levels of Bax, Caspase-3 and MMP-9 were observed in the VALP group, while RUT treatment decreased this value. When the spermatological analyzes were examined, it was observed that the total motility value decreased significantly in the VALP group, but the total motility value increased in the RUT treatment group (p<0.05). A significant increase in the rate of dead and abnormal sperm was found in the VALP group, but these parameters improved in the RUT treatment group. As a result, RUT has protective effects against VALP-induced testicular damage in rats.

Keywords

• Oxidative stress
• rat
• rutin
• semen
• valproic acid.

Kaynakça

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