Validation of A Portable Beta-Hydroxybutyric Acid Analyser for The Diagnosis of Subclinical Ketosis in Dairy Cows

Öz

On-farm determination of beta-hydroxybutyric acid (BHBA) with an accurate analyser is important for rapid diagnosis of subclinical ketosis (SCK) or clinical ketosis in dairy cows. The accuracy of the Taidoc BHBA portable analyser has not yet been tested and validated. The objective was the validation of the Taidoc BHBA analyser with another device Wellion (already validated) in 84 dairy cows. Whole blood was collected from the coccygeal vein and immediately analysed with Taidoc and Wellion in one drop of whole venous blood of 84 dairy cows. The averages of BHBA concentration were 0.79±0.66 and 1.09±0.51 mmol.l-1 for Wellion and Taidoc, respectively. The correlation coefficient was r=0.64 (p<0.001). Method comparison by the Passing-Bablok regression equation (y=0.0664+0.06111x) showed no agreement between the two instruments. The intercept A and slope B did not include 0.0 and 1.0, respectively. The Bland-Altman plots of agreement indicated that the mean deviation was 0.30 mmol.l-1 and the total deviation was 2.02 mmol.l-1, which was unacceptably high. The weighted kappa value was 0.26, indicating minimal agreement. AUC, sensitivity and specificity for the diagnosis of SCK at cut-off point ≥1.2 mmol.l-1 BHBA were 0.78, 64.7% and 91.0%, respectively. There was no agreement between the Taidoc and the Wellion BHBA portable analyser for dairy cows and they cannot be used interchangeably. Taidoc gave much higher BHBA values than Wellion and its sensitivity and specificity for SCK diagnosis was not comparable to Wellion.

Anahtar Kelimeler

• Beta-hydroxybutyric acid
• Dairy cow
• Subclinical ketosis
• Taidoc
• Wellion

Süt İneklerinde Subklinik Ketozis Teşhisi İçin Portatif Beta-Hidroksibütirik Asit Analiz Cihazının Validasyonu

Öz

Süt çiftliklerinde beta-hidroksibütirik asidin (BHBA) doğru bir cihazla ölçülmesi, subklinik ketozisin (SCK) veya klinik ketozisin hızlı teşhisi için önemlidir. Taidoc BHBA taşınabilir analiz cihazının doğruluğu henüz valide edilmemiştir. Bu çalışma, Taidoc BHBA analiz cihazını valide etmek için daha önceden validasyonu yapılmış Wellion cihazı ile 84 süt ineğinde karşılaştırılarak yapılmıştır. Süt ineklerinde V. coccygea’dan alınan bir damla kan Taidoc ve Wellion ile analiz edilmiştir. BHBA konsantrasyonlarının ortalamaları Wellion ve Taidoc için sırasıyla 0,79±0,66 ve 1,09±0,51 mmol.l-1 olarak bulunmuştur. İki cihaz arasında korelasyon katsayısı r=0,64 (p<0,001) çıkmıştır. Passing-Bablok regresyon denklemiyle (y=0,0664+0,06111x) yöntem karşılaştırması, iki cihaz arasında bir uyum olmadığını gösterdi. Intercept ve slope sırasıyla 0.0 ve 1.0'ı içermemiştir. Bland-Altman uyum grafikleri, ortalama sapmanın 0,30 mmol.l-1 ve toplam sapmanın 2,02 mmol.l-1 olduğunu gösterdi ki, bu kabul edilemeyecek kadar yüksek çıkmıştır. Ağırlıklı kappa değeri 0.26 çıkmış ve bu değer minimum derecede bir uyumu göstermiştir. SCK’nın teşhisi için BHBA≥1,2 mmol.l-1 kesme noktasında eğri altındaki alan, duyarlılık ve özgüllük sırasıyla 0,78, %64,7 ve %91,0 çıkmıştır. Süt inekleri için Taidoc ve Wellion BHBA taşınabilir analiz cihazları arasında bir uyum bulunmamış ve cihazlar birbirlerinin yerine kullanılamazlar. Taidoc, Wellion'dan çok daha yüksek BHBA değerleri vermiştir ve SCK teşhisi için duyarlılığı ve özgüllüğü Wellion ile karşılaştırılabilir oranda değildir.

Anahtar Kelimeler

• Beta-hidroksibütirik asit
• Süt ineği
• Subklinik ketozis
• Taidoc
• Wellion

Kaynakça

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