Rapid Confirmation Analysis of Multiple Antibiotic Residues in Honey by LC-MS/MS

Öz

Honey is a natural product containing high levels of sugar, amino acids, minerals, enzymes and vitamins and has been used as food and medicine for thousands of years. In terms of public health, honey should not contain any chemicals that may pose a health risk. Both pesticides used in agricultural areas around the apiary and drugs used in the treatment of bee diseases cause residue problems in honey. In the study, it was aimed to develop a method for rapid confirmation analysis of multiple antibiotic residues in honey samples using a two-stage liquid-liquid extraction method. With the developed method, sulphonamide (n:11), fluoroquinolone (n:2), phenicol (n:2) tetracycline (n:2) and macrolide (n:2) were used in honey samples. Screening and confirmation analyses can be performed for 19 antibiotic residues in 5 different antibiotic groups. The detection limits of the method are between 5.29 and 10.58 µg.kg-1, the accuracy values are between 86.9% and 119%, the coefficient of variation is between 1.26% and 16.33% and recovery is between 84% and 117.23%.

Anahtar Kelimeler

• Antibiotic
• Confirmation
• Honey
• LC-MS/MS
• Residue

Balda Çoklu Antibiyotik Kalıntılarının LC-MS/MS ile Hızlı Doğrulama Analizi

Öz

Bal, yüksek oranda şeker, aminoasit, mineral madde, enzim ve vitamin içeren, binlerce yıldır gıda ve ilaç olarak kullanılan doğal bir üründür. Halk sağlığı açısından, balda sağlık riski oluşturabilecek herhangi bir kimyasal maddenin bulunmaması gerekir. Gerek arılık çevresindeki tarımsal alanlarda kullanılan pestisitler, gerekse arı hastalıklarının tedavisinde kullanılan ilaçlar, bal da kalıntı sorunlarının yaşanmasına neden olmaktadır. Çalışmada, iki farklı likit-likit ekstraksiyon metodu kullanılarak bal örneklerinde çoklu antibiyotik kalıntılarının hızlı doğrulama analizleri için metot geliştirilmesi amaçlanmıştır. Geliştirilen metotla, bal örneklerinde, sulfonamid (n:11), flourokinolon (n:2), fenikol (n:2) tetrasiklin (n:2) ve makrolid (n:2) olmak üzere 5 farklı antibiyotik grubunda 19 adet antibiyotik kalıntısı için tarama ve doğrulama analizleri yapılabilir. Metodun tespit limitleri 5,29 ile 10,58 µg.kg-1, doğruluk değerleri %86,9 ile %119 varyasyon katsayı değerleri %1,26 ile %16,33 ve geri kazanım oranları %85,4 ile %117,23 arasındadır.

Anahtar Kelimeler

• Antibiyotik
• Bal
• Doğrulama
• Kalıntı
• LC-MS/MS

Kaynakça

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