Goat Sperm Vitrification

Öz

The vitrification method is a freezing method in which the cooling rate is very high and high concentration permeable cryoprotectants are used. The vitrification method, which has a wide application area in embryo freezing, was applied to the storage of goat semen and the effect of the vitrification method on semen quality was determined. For this purpose, semen samples taken from 4 healthy goats via artificial vagina were divided into 4 equal groups. While the semen samples in Group 1 were frozen with the conventional method with Tris-sitrate modified solution containing 20% egg yolk and 5% glycerol, the remaining sperm samples were frozen with 0.1 M (Group II), 0.25 M (Group III) and 0.5 M (Group IV) was diluted with Human Tubal Fluid diluent containing sucrose and vitrification method was applied. Standard semen analysis (spermatozoa motility, plasma membrane integrity, sperm morphology, chromatin integrity) was performed on all semen samples after conventional freezing and vitrification. As a result, attempts to vitrify goat spermatozoa using non-permeable cryoprotectants have resulted in very low viability. The physical and chemical changes occurring during vitrification negatively affected the motility, viability and sperm morphology of spermatozoa.

Anahtar Kelimeler

• Goat
• Semen
• Sucrose
• Vitrification

Proje Numarası

1609

Teke Spermasının Vitrifikasyonu

Öz

Vitrifikasyon metodu, soğutma hızının çok yüksek olduğu, yüksek konsatrasyonlu permeable kriyopektanların kullanıldığı bir dondurma yöntemidir. Embriyo dondurulmasında oldukça geniş uygulama alanı bulan vitrifikasyon methodunun teke spermasının saklanmasında uygulanarak sperma kalitesi üzerine vitrifikasyon methodunun etkisi belirlenmiştir. Bu amaçla 4 sağlıklı tekeden suni vajen yoluyla alınan sperma örnekleri 4 eşit gruba ayrılmıştır. Grup 1’deki sperma örnekleri %20 yumurta sarısı ve %5 gliserol içeren Tris-Sitrat modifiye solüsyonu ile konvansiyonel methodla dondurulurken, geri kalan sperm örnekleri ise 0.1 M (Grup 2), 0.25 M (Grup 3) ve 0.5 M (Grup 4) sükroz içeren Human Tubal Fluid sulandırıcısı ile sulandırılıp vitrifikasyon işlemine tabi tutulmuştur. Tüm sperma örneklerinde konvansiyonel dondurma ve vitrifikasyon işlemi sonrası standart sperma analizi (spermatozoa motilitesi, plasma membran bütünlüğü, sperm morfolojisi, kromatin bütünlüğü) yapılmıştır. Sonuç olarak, non-permeable kriyoprotektanlar kullanılarak teke spermatozoasını vitrifiye etmek için girişilen deneyimler çok düşük canlılıkla sonuçlanmıştır. Vitrifikasyon sırasında oluşan fiziksel ve kimyasal değişiklikler, spermatozoanın motilitesini, canlılığını, sperm morfolojisini olumsuz şekilde etkilemiştir.

Anahtar Kelimeler

• Sperma
• Sükroz
• Teke
• Vitrifikasyon

Destekleyen Kurum

Harran Üniversitesi

Proje Numarası

1609

Etik Beyan

Alınmıştır.

Teşekkür

HÜBAP

Kaynakça

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