Pit1/Hinfi Polymorphism in Holstein Cattle in Afyonkarahisar Province

Öz

Pit1 (Pituitary Specific Transcription Factor-1) gene has been shown to be resided on the centromeric region of chromosome 1 in cattle. This gene, also known as POUF1F has responsible for development of pituitary gland and hormone expression in mammals. Pit1 deficiency causes a dwarfism phenotype in mice, dogs and fish. Previous studies have demonstrated to associated with polymorphisms of the Pit1 gene and growth and reproductive performance and milk, meat yield traits. In the cattle, a HinfI polymorphism on Pit1 gene (c.1178G>A) have been reported that there is an association between milk and reproduction yield parameters. In this study, we aimed existence and distribution of Pit1/HinfI polymorphism in 81 head Holstein cattle in Afyonkarahisar province. In our study, we defined that there were 5 AA, 30 AG and 46 GG genotypes in our survey population. We also detected to frequency of A allele as 0.25 and the G allele as 0.75. We calculated to genetic index values such as PIC (0.3027418) and Heterozygosity (0.3742044) using RStudio package. Chi-square value was found 0.049 and was exhibited to survey population in Hardy-Weinberg equilibrium. The Pit1/HinfI polymorphism is a potential option for use in marker-assisted selection studies in light of these findings for Holstein cattle in Afyonkarahisar province.

Anahtar Kelimeler

• HinfI
• Holstein sığır
• PCR-RFLP
• Pit1

Afyonkarahisar’da Yetiştirilen Holstein Sığırlarda Pit1/Hinfi Polimorfizmi

Öz

Pit1 (Hipofize Özgü Transkripsiyon Faktörü-1) Sığırlarda 1.kromozomun sentromerik bölgesinde bulunan yaklaşık 129 aminoasitten oluşan bir protein kodlayan bir gendir. Memelilerde hipofiz bezinin gelişiminden ve hormon ekspresyonundan sorumlu olan Pit1 geni, POUF1F olarak da bilinmektedir. Pit1 eksikliğinde farelerde, köpeklerde, balıklarda cücelik fenotipi gözlenmektedir. Yapılan çalışmalar, Pit1 genindeki polimorfizmlerin sığırlarda, koyunlarda, domuzlarda, tavuklarda büyüme, üreme performansı, et ve süt verimi özellikleriyle ilişkili olduğunu göstermiştir. Sığırlarda Pit1/HinfI (c.1178G>A) polimorfizminin süt verimi ve döl verimi parametreleriyle ilişkili olduğu ifade edilmektedir. Bu çalışmada, Afyonkarahisar ilinde yetiştirilen 81 baş Holstein sığır Pit1/HinfI polimorfizminin varlığı ve dağılımının belirlenmesi amaçlanmıştır. PCR-RFLP tekniği kullanılarak hayvanların bu polimorfizm açısından genotiplendirilmiştir. Çalışma sonucunda 81 hayvandan 5 tanesinin AA, 30 tanesinin AG ve 46 tanesinin GG genotipinde olduğu bulunmuştur. G allelinin frekansı 0,75, A allelinin frekansı ise 0,25 olarak hesaplanmıştır. Genetik indeks değerlerinden PIC değeri 0,3027418 ve Heterozigotluk değeri 0,3742044 olarak hesaplanmıştır. χ2 değeri 0,049 bulunmuş olup, popülasyonun Hardy-Weinberg dengesinde olduğu ortaya konulmuştur. Bu bulgular ışığında Pit1/HinfI polimorfizminin Afyonkarahisar ilinde yetiştirilen Holstein sığırlar için markör destekli seleksiyon çalışmalarında kullanılabilecek potansiyel bir aday olduğu düşünülmektedir.

Anahtar Kelimeler

• HinfI
• Holstein sığır
• PCR-RFLP
• Pit1

Kaynakça

1. Anggraeni, A., Sumantri, C., Saputra, F., & Praharani, L. (2020). Association between GH (g. 1456_1457insT), GHRH (g. 4474 C> A), and Pit-1 (g. 244G> A) polymorphisms and lactation traits in Holstein Friesian cattle. Tropical Animal Science Journal, 43(4), 291-299.
2. Ardicli, S., Dincel, D., Samli, H., Senturk, N., Karalar, B., Unlu, S., Soyudal, B., Kubad, E., & Balci, F. (2023). Association of polymorphisms in lipid and energy metabolism-related genes with fattening performance in Simmental cattle. Animal biotechnology, 34(8), 3428-3440.
3. Aytekin, I., & Fadhil, M. (2022). Pit-1 Gene Polymorphisms in Anatolian Black, Holstein Friesian, Brown Swiss and Simmental Cattle Reared in Turkey. Journal of Agricultural Sciences, 28(1), 139-144.
4. Aytekin, I., & Boztepe, S. (2013). Associations of Pit-1 gene polymorphism with milk yield and composition traits in brown swiss cattle. J Anim Plant Sci, 23(5), 1281-1289.
5. Bai, J. Y., Wang, X., Yang, Y. B., Zhang, X. H., Pang, Y. Z., & Li, H. W. (2016). Study on the polymorphism of POU1F1 gene in sheep. Revista Brasileira de Zootecnia, 45, 604-607.
6. Bayram, D., Arslan, K., Akyüz, B., & Ișcan, K. M. (2017). Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey.
7. Botstein, D., White, R. L., Skolnick, M., & Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 32(3), 314.
8. Chauhan, A., Tiwari, M., Singh, S. P., Sharma, D., Kumar, S., Goel, R., ... & Singh, V. (2015). Association of PIT-1 gene polymorphism with milk production traits in Sahiwal cattle. Indian Journal of Animal Sciences, 85(6), 610-612.

9. Cohen, L. E., Wondisford, F. E., & Radovick, S. (1996). Role of Pit-1 in the gene expression of growth hormone, prolactin, and thyrotropin. Endocrinology and metabolism clinics of North America, 25(3), 523-540.
10. Doosti, A., Arshi, A., & Momeni, B. (2011). Molecular study of PIT1 gene polymorphism in Holstein and Iranian native cattle. African Journal of Agricultural Research, 6(19), 4467-4470.
11. De Mattos, K. K., Del Lama, S. N., Martinez, M. L., & Freitas, A. F. (2004). Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesquisa Agropecuária Brasileira, 39, 147-150.
12. Dybus, Andrzej, Szatkowska, Iwona, Czerniawska-Piątkowska, E., Grzesiak, Wilhelm., Wójcik, Jerzy, Rzewucka, Edyta, & Zych, Slawomir. (2004). PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle. Archives Animal Breeding, 47(6), 557-563.
13. Edriss, V., Edriss, M. A., Rahmani, H. R., & Sayed-Tabatabaei, B. E. (2008). Pit-1 gene polymorphism of Holstein cows in Isfahan Province. Biotechnology, 7(2), 209-212.
14. Flurkey, K., Papaconstantinou, J., & Harrison, D. E. (2002). The Snell dwarf mutation Pit1dw can increase life span in mice. Mechanisms of ageing and development, 123(2-3), 121-130.
15. Gritsienko, Y. V., Gill, M. I., Denisyuk, L., & Gorbatenko, I. Y. (2020, November). Polymorphism of genes of the protein and lipid exchanges in modern Ukranian breeds of cattle bred for dairy productivity. In Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. (Vol. 74, No. 6, pp. 373-380).
16. Hartati, H., Anwar, S., & Soewandi, B. D. P. (2018). Genetic polymorphism of Pit-1| HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station. Journal of the Indonesian Tropical Animal Agriculture, 43(4), 315-322.
17. Hendriks-Stegeman, B. I., Augustijn, K. D., Bakker, B., Holthuizen, P., van der Vliet, P. C., & Jansen, M. (2001). Combined pituitary hormone deficiency caused by compound heterozygosity for two novel mutations in the POU domain of the Pit1/POU1F1 gene. The Journal of Clinical Endocrinology & Metabolism, 86(4), 1545-1550.
18. Hoseinzadeh, Z. E., Mohammadabadi, M. R., Esmailizadeh, A. K., & Khezri, A. (2015a). Association of PIT1 gene and milk protein percentage in Holstein cattle. J Livest Sci Tech, 31(31), 40-9.
19. Hoseinzadeh, Z. E., Mohammadabadi, M. R., Koshkuieh, A. E., Khezri, A., & Noori, A. N. (2015b). Association of PIT1 gene with milk fat percentage in Holstein cattle.
20. Ingraham, H. A., Flynn, S. E., Voss, J. W., Albert, V. R., Kapiloff, M. S., Wilson, L., & Rosenfeld, M. G. (1990). The POU-specific domain of PIT-1 is essential for sequence-specific, high affinity dna binding and DNA-dependent Pit-1—Pit-1 interactions. Cell, 61(6), 1021-1033.
21. Khaizaran, Z. (2014). Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism.
22. Lantinga-van Leeuwen, I. S., Mol, J. A., Kooistra, H. S., Rijnberk, A., Breen, M., Renier, C., & van Oost, B. A. (2000). Cloning of the canine gene encoding transcription factor Pit-1 and its exclusion as candidate gene in a canine model of pituitary dwarfism. Mammalian Genome, 11, 31-36.
23. Li, S., Crenshaw III, E. B., Rawson, E. J., Simmons, D. M., Swanson, L. W., & Rosenfeld, M. G. (1990). Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1. Nature, 347(6293), 528-533.
24. Mangalam, H. J., Albert, V. R., Ingraham, H. A., Kapiloff, M., Wilson, L., Nelson, C., ... & Rosenfeld, M. G. (1989). A pituitary POU domain protein, Pit-1, activates both growth hormone and prolactin promoters transcriptionally. Genes & development, 3(7), 946-958.
25. Misrianti, R., Sumantri, C., & Farajallah, A. (2010). Polymorphism identification of Pit1 gene in Indonesian buffaloes (Bubalus bubalis) and Holstein-Friesian cows. Media Peternakan, 33(3), 131-131.
26. Moravcíková, N., Trakovická, A., Miluchová, M., Bujko, J., & Navrátilová, A. (2013). HinfI polymorphism of PIT-1 gene in Slovak spotted cattle. The Journal of Microbiology, Biotechnology and Food Sciences, 1883.
27. Moody, D. E., Pomp, D., & Barendse, W. (1995). Restriction fragment length polymorphism in amplification products of the bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Animal genetics, 26(1), 45-47.
28. Nica, G., Herzog, W., Sonntag, C., & Hammerschmidt, M. (2004). Zebrafish pit1 mutants lack three pituitary cell types and develop severe dwarfism. Molecular Endocrinology, 18(5), 1196-1209.
29. Nie, Q., Fang, M., Xie, L., Zhou, M., Liang, Z., Luo, Z., Wang, G., Bi, W., Liang, C., Zhang W., & Zhang, X. (2008). The PIT1 gene polymorphisms were associated with chicken growth traits. BMC genetics, 9, 1-5.
30. Piorkowska, K., Ropka-Molik, K., Oczkowicz, M., Różycki, M., & Żukowski, K. (2013). Association study of PIT1 and GHRH SNPs with economically important traits in pigs of three breeds reared in Poland.
31. Pozovnikova, M. V., Rotar, L. N., Kudinov, A. A., & Dementieva, N. V. (2020). The linkage of polymorphic variants of genes Gh, Prl, and Pit-1 and milk productivity of cows with morphology of cumulus-oocyte complex sampled post mortem. Cytology and Genetics, 54, 212-219.
32. Pytlewski, J., Antkowiak, I., & Czerniawska-Piatkowska, E. (2018). Relationship of PIT-1 gene polymorphism with breeding parameters and body weights of cows and calves. Pakistan Journal of Zoology, 50(1).
33. Pytlewski, J., Antkowiak, I. R., & Czerniawska-Piątkowska, E. (2022). Polymorphism of PIT-1 genes and its relationship with traits in the Limousine cattle breed.
34. Renaville, R., Gengler, N., Vrech, E., Prandi, A., Massart, S., Corradini, C., Bertozzi, C., Mortiaux, F., Burny, A., & Portetelle, D. (1997). Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. Journal of Dairy Science, 80(12), 3431-3438.
35. Sakar, Ç. M., & Zülkadir, U. (2022). Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene. Animal Biotechnology, 33(3), 536-545.
36. Selvaggi, M., Dario, C., Normanno, G., Dambrosio, A., & Dario, M. (2011). Analysis of two pit-1 gene polymorphisms and relationships with growth performance traits in Podolica young bulls. Livestock Science, 138(1-3), 308-312.
37. Selvaggi, M., & Dario, C. (2011). Analysis of two Pit-1 gene polymorphisms: Single nucleotide polymorphisms (SNPs) distribution patterns in Podolica cattle breed. African Journal of Biotechnology, 10(55), 11360-11364.
38. Sönmez, Z., & Ünal, H. (2023). The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle. Yuzuncu Yıl University Journal of Agricultural Sciences, 33(4), 513-521.
39. Supowit, S. C., Ramsey, T., & Thompson, E. B. (1992). Extinction of prolactin gene expression in somatic cell hybrids is correlated with the repression of the pituitary-specific trans-activator GHF-1/Pit-1. Molecular Endocrinology, 6(5), 786-792.
40. Taipova A., Beishova A., I., Alikhanov, K., & Belaya, E. (2020). Evaluation Of Polymorphic Variants Of Genes Of Somatotropin Cascade As Genetic Markers Of Meat Productivity In Domestic Cattle Breed.
41. Thuy, N. T. D., Thu, N. T., Cuong, N. H., Ty, L. V., Nguyen, T. T. B., & Khoa, D. V. A. (2018). Polymorphism of PIT-1 and prolactin genes and their effects on milk yield in Holstein Frisian dairy cows bred in Vietnam. Russian journal of genetics, 54, 346-352.
42. Trakovická, A., Moravčíková, N., Minarovič, T., & Navrátilová, A. (2015). SNPs analyses of the bovine LEP and PIT-1 genes by multiplex PCR-RFLP method and their effect on milk performance traits in Slovak Simmental cattle. Journal of Central European Agriculture.
43. Toyğar, O., & Ozdemir, M. (2022). The relationships between Pit-1 gene polymorphism and some performance traits in Simmental and Brown Swiss breeds. Journal of the Hellenic Veterinary Medical Society, 73(4), 4689-4696.
44. Tuggle, C. K., & Trenkle, A. (1996). Control of growth hormone synthesis. Domestic Animal Endocrinology, 13(1), 1-33.
45. Woollard, J., Schmitz, C. B., Freeman, A. E., & Tuggle, C. K. (1994). Rapid communication: HinfI polymorphism at the bovine Pit-1 locus. Journal of animal science, 72(12).
46. Zakizadeh, S., Reissmann, M., Rahimi, G., Javaremi, A. N., Reinecke, P., Mirae-Ashtiani, S. R., & Shahrbabak, M. M. (2007). Polymorphism of the bovine POU1F1 gene: allele frequencies and effects on milk production in three Iranian native breeds and Holstein cattle of Iran. Pakistan Journal of Biological Sciences: PJBS, 10(15), 2575-2578.
47. Zghairand, Z. S., & Hassooni, H. A. (2021, November). The Relationship Between The PIT-1 Gene at The Rate of Daily Milk Production and Some of Its Components in Buffaloes. In IOP Conference Series: Earth and Environmental Science (Vol. 923, No. 1, p. 012029). IOP Publishing.
48. Zhang∗, C., Liu∗, B., Chen, H., Lan, X., Lei, C., Zhang, Z., & Zhang, R. (2009). Associations of a Hinf I PCR-RFLP of POU1F1 gene with growth traits in Qinchuan cattle. Animal Biotechnology, 20(2), 71-74.
49. Zhou, F., Yang, Q., Lei, C., Chen, H., & Lan, X. (2016). Relationship between genetic variants of POU1F1, PROP1, IGFBP3 genes and milk performance in Guanzhong dairy goats. Small Ruminant Research, 140, 40-45.