Sıçanlarda Glifosat İzopropilamin ile Oluşturulan Testiküler Hasara Karşı Klorojenik Asidin Koruyucu Etkisi

Öz

Bu subkronik toksisite çalışmasının amacı, testis dokularında Glifosat izopropilamin (GLF-ISO) tarafından indüklenen histopatolojik-histolojik değişiklikler ve oksidatif stres üzerinde klorojenik asidin (CGA) profilaktik etkisini belirlemektir. Toplam 42 erkek Wistar sıçan, her biri 7 sıçan içeren altı eşit gruba ayrılmıştır. Ön muamele için sıçanlara 7 hafta boyunca 12.5, 25 ve 50 mg/kg (po) dozlarında CGA ve 787.85 mg/kg (po) dozunda GLF-ISO verilmiştir. GLF-ISO testis dokularında malondialdehit seviyelerini önemli ölçüde artırırken SOD ve CAT aktivitelerini ve GSH seviyelerini azaltmıştır. Aksine, bu parametreler CGA ile tedavi edilen gruplarda iyileşmiştir. Ayrıca, CGA, GLF-ISO tarafından indüklenen testis dokularındaki histopatolojik ve histolojik değişiklikleri doza bağlı bir şekilde iyileştirmiştir. Sonuçlar, GLF-ISO'nun neden olduğu testis hasarının CGA ile potansiyel olarak önlenebileceğini veya yönetilebileceğini göstermektedir.

Anahtar Kelimeler

• Glyphosate İzopropilamin
• Klorojenik asit
• Oksidatif stres
• Testiküler hasar

Protective Effect of Chlorogenic Acid Against Testicular Damage Induced By Glyphosate Isopropilamine in Rats

Öz

The aim of this subchronic toxicity study was to determine the prophylactic effect of chlorogenic acid (CGA) on the histopathologic-histologic changes and oxidative stress induced by Glyphosate isopropylamine (GLF-ISO) in testicular tissues. A sum of 42 male Wistar rats were divided into six equal groups, each containing 7 rats. For pretreatment, rats were given CGA at doses of 12.5, 25 and 50 mg/kg (po) and GLF-ISO at 787.85 mg/kg (po) for 7 weeks. GLF-ISO significantly increased malondialdehyde levels while decreasing SOD and CAT activities and GSH levels in testicular tissues. On the contrary, these parameters were improved in CGA-treated groups. Furthermore, CGA ameliorated the histopathological and histological changes in testicular tissues induced by GLF-ISO in a dose-dependent manner. The results indicate that testicular damage caused by GLF-ISO can potentially be prevented or managed by CGA.

Anahtar Kelimeler

• Glyphosate isopropilamine
• Chlorogenic acid
• Oxidative stress
• Testicular damage

Destekleyen Kurum

Afyon Kocatepe University Scientific Research Projects Coordinators

Etik Beyan

This study was carried out at Afyon Kocatepe University Reserch Animals Application Center. This research was approved by The Ethics Committee of the Faculty of Veterinary Medicine, Afyon Kocatepe University (AKUHADYEK, Ref No: 49533702/65, Tarih: 04/2018)

Teşekkür

In this study would like to thank the Afyon Kocatepe University Scientific Research Projects Coordinators with the study entitled “Evaluation of the therapeutic efficacy of single and combined use of crocin, curcumin, and chlorogenic acid against intoxication induced by glyphosate isopropylamine in rats,” Project No: 18.KARİYER.244, for their contributions to the present study and also thank the administration and staff of the Afyon Kocatepe University Research Animals Application Center.

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