Enerjilendirilmiş Oksijen Türevi ve Hodan Yağı İçeren Bitkisel Formülasyonun Etkinliğinin İn Vitro ve İn Vivo Çalışmalar ile Değerlendirilmesi

Öz

Tıbbi ve aromatik bitkiler çok eski çağlardan bu yana tedavi amacıyla kullanılmaktadır. Teknolojik gelişmelere paralel olarak bitkisel içerikli medikal ürünlere olan ilgi giderek artmaktadır. İleri formülasyon teknikleriyle geliştirilen bu ürünler, yara ve yanıkların tedavisinde güvenilir, hızlı ve etkili bir şekilde kullanılabilmektedir. Bu çalışmada, hodan yağının sitotoksisitesi insan dermal fibroblast hücreleri üzerinde in vitro olarak değerlendirilmiştir. Hodan yağı ve enerjilendirilmiş oksijen molekülü içeren bitkisel içerikli formülasyonun ikinci derece in vivo yanık modellemesi üzerindeki etkinliği belirlenmiştir. Sitotoksisite test sonuçları Hodan yağının insan dermal fibroblast hücreleri üzerinde toksik bir etkisinin bulunmadığını göstermiştir. Bitkisel formülasyonun ikinci derece yanıklarda kontrol grubuna göre anlamlı düzeyde etkili olduğu belirlenmiştir. Bitkisel formülasyonun yara iyileştirici etkinliğinin Hodan yağının antimikrobiyal, antifungal, antioksidan ve hücre proliferasyonunu artırıcı etkisi sayesinde gerçekleştiği düşünülmektedir. Elde edilen sonuçlar; bitkisel formülasyonun ikinci derece yanıkların tadavisinde kullanılabilme potansiyeli olduğunu göstermiştir.

Anahtar Kelimeler

• Enerjilendirilmiş oksijen molekülleri
• Hodan yağı
• Yanık

Evaluation of the Efficacy of a Herbal Formulation Containing Energised Oxygen Derivative and Borage Oil by In Vitro and In Vivo Studies

Öz

The use of medicinal and aromatic plants for therapeutic purposes dates back to ancient times. In parallel with technological developments, there has been a notable increase in interest in botanical-based medicinal products. The products developed using advanced formulation techniques are safe, effective and rapid in the treatment of wounds and abrasions. In this study, the cytotoxicity of borage oil was evaluated in vitro on human dermal fibroblast cells. The effectiveness of a herbal formulation containing borage oil and energized oxygen molecules on second-degree in vivo burn modeling was determined. Acording to cytotoxicity test results showed that Borage oil did not have a toxic effect on human dermal fibroblast cells. It was determined that the herbal formulation was significantly more effective in second-degree burns than the control group. It is thought that the wound healing effect of the herbal formulation occurs thanks to the antimicrobial, antifungal, antioxidant and cell proliferation-increasing effects of Borage oil. The results obtained showed that the herbal formulation has the potential to be used in the treatment of second-degree burns.

Anahtar Kelimeler

• Energized oxygen molecules
• Borage oil
• Burn

Kaynakça

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