İvesi Irkı Koyunlarda Prepartum Vitamin E/Selenyum ve Melatonin Tedavisinin Uterus İnvolüsyonu Üzerine Olan Etkileri

Öz

Bu çalışmanın amacı, prepartum dönemde Vitamin E, Selenyum ve Melatonin uygulamalarının İvesi ırkı koyunlarında uterus involüsyonu üzerine etkilerini araştırmaktı. Çalışmaya tekil gebeliğe sahip toplam 24 koyun dahil edildi. Beklenen doğum zamanlarından 15 gün önce koyunlar rastgele üç gruba ayrıldı. Grup VE’deki koyunlara (n=8) intramüsküler yoldan Vitamin E ve selenyum uygulandı. Grup M’deki koyunlara (n=8) 18 mg melatonin içeren subkutan kulak implantı uygulandı. Grup C’deki koyunlar (n=8) ise kontrol grubu olarak kullanıldı ve bu gruba herhangi bir tedavi uygulanmadı. Uterus involüsyonlarının tamamlanma süresi Grup VE, Grup M ve Grup C için sırasıyla 20,57±1,21; 19,13±1,13; 18,67±1,09 gün olarak tespit edildi (p>0,05). Uterus çapları Grup VE, Grup M ve Grup C için sırasıyla 9,66±0,59; 9,54±0,35; 7,95±0,22 olarak belirlendi. Doğum sonrası 3. günde Grup VE ve Grup M'deki uterus çapları, Grup C'den anlamlı derecede büyük tespit edildi. (p<0,05). Ortalama dış karunkular çapları Grup VE, Grup M ve Grup C için sırasıyla 1,93±0,10; 1,60±0,10; 1,65±0,07 olarak ölçüldü. Grup VE'deki ortalama dış karunkular çapları, Grup M ve Grup C'den anlamlı derecede büyük olduğu görüldü (p<0,05). Gruplardan bağımsız olarak; 3. gündeki ortalama dış karunkular çapları, 6. güne kıyasla daha büyük olarak tespit edildi (p<0,05). Aynı şekilde 3. gündeki iç karunkular çapları da 6. güne kıyasla daha büyük olarak tespit edildi (p<0,05). Gruplar arasında ortalama iç karunkular çapları açısından fark tespit edilmedi (p>0,05). Sonuç olarak, prepartum dönemde Vitamin E, Selenyum ve Melatonin uygulamasının, İvesi koyunlarında postpartum uterus involüsyon süresinin tamamlanması üzerinde önemli bir etkisinin olmadığı görüldü.

Anahtar Kelimeler

• koyun
• melatonin
• selenyum
• uterus involüsyonu
• Vitamin E

The Effects of Prepartum Vitamin E/Selenium and Melatonin Treatment on Uterine Involution of Awassi Sheep in Hatay

Öz

The aim of this study was to investigate the effects of prepartum Vitamin E, Selenium and Melatonin treatments on uterine involution in Awassi sheep. The study included a total of 24 ewes with singleton pregnancies. Fifteen days before their expected delivery, ewes were randomly divided into three groups. Group VE (n=8) received Vitamin E and Selenium intramuscularly. Group M (n=8) received a subcutaneous ear implant containing 18 mg of melatonin. Group C (n=8) served as the control group and received no treatment. The mean time for completion of uterine involution in Group VE, Group M, and Group C were 20.57±1.21; 19.13±1.13; 18.67±1.09 days, respectively (p>0.05). Uterine diameters were 9.66±0.59; 9.54±0.35; 7,95±0.22 in Group VE, Group M, and Group C, respectively. Uterine diameters in Group VE and Group M were significantly greater than Group C on Day 3 of postpartum (p<0.05). Mean outer caruncular diameters were 1.93±0.10; 1.60±0.10; 1.65±0.07 in Group VE, Group M, and Group C. respectively. Mean outer caruncular in Group VE were significantly greater than Group M and Group C (p<0.05). Mean outer caruncular diameters were greater on Day 3 compared to Day 6 regardless of groups (p<0.05). Mean inner caruncular diameters were greater on Day 3 compared to Day 6 regardless of groups (p<0.05). There was no differences in terms of mean inner caruncular diameters between groups (p>0.05). In conclusion, it was found that prepartum administration of Vitamin E, Selenium and Melatonin had no significant effects on the completion time of postpartum uterine involution on Awassi ewes.

Anahtar Kelimeler

• melatonin
• selenium
• sheep
• uterine involution
• Vitamin E

Kaynakça

1. Abdel-Raheem, S. M., Mahmoud, G. B., Senosy, W., & El-Sherry, T. M. (2019). Influence of vitamin E and selenium supplementation on the performance, reproductive indices and metabolic status of Ossimi ewes. Slovenian Veterinary Research, 56 (Suppl 22), 353-363. https://doi.org/10.26873/SVR-774-2019
2. Abecia, J. A., Forcada, F., Vázquez, M. I., Muiño-Blanco, T., Cebrián-Pérez, J. A., Pérez-Pe, R., & Casao, A. (2019). Role of melatonin on embryo viability in sheep. Reprod Fertil Dev, 31(1), 82-92. https://doi.org/10.1071/RD18308
3. Abecia, J. A., Lozano, J. M., & Forcada, F. (1999). A preliminary study on the effects of dietary energy and melatonin on the ex vivo production of progesterone and prostaglandin F2α by the corpora lutea and endometrial tissue of ewes. Vet Res Commun, 23, 115-121. https://doi.org/10.1023/A:1006206519001
4. Afzaal, M., Khan, M., Ahmad, W., Hayat, A., Abbas, Q., Arshad, M., ... & Riaz, A. (2023). Effects of prepartum melatonin treatment and suckling on postpartum reproductive performance of Beetal goats under subtropical conditions. Small Rum Res, 221, 106936. https://doi.org/10.1016/j.smallrumres.2023.106936
5. Ahmed, M. N., Ynzeel, J. H., & Majeed, A. F. (2016). Ultrasonographic study of uterine involution in of Awassi ewes in Iraq. Al-Anbar J Vet Sci, 9(1), 16-21.
6. Alalaf, A. S., & Alnuaimy, R. (2024). Evaluating The Influence of Vitamin E and Selenium on The Uterine Health of Adult Female Goats: A Histological Study. Egyptian J Vet Sci, 1-9. https://doi.org/10.21608/EJVS.2024.285414.2038
7. Barcelos, B., Gomes, V., Vidal, A. M. C., de Freitas Júnior, J. E., de Araújo, M. L. G. M. L., Alba, H., & Netto, A. S. (2022). Effect of selenium and vitamin E supplementation on the metabolic status of dairy goats and respective goat kids in the peripartum period. Trop Anim Health Prod, 54, 1-13. https://doi.org/10.1007/s11250-021-03034-1
8. Dal, G. E., Enginler, S. O., Cetin, A. C., Baykal, K., & Sabuncu, A. (2020). B-mode and Doppler Ultrasonographic Assessment of Uterine Involution in Ewes Treated with Two Different Doses of ProstaglandinF2α. Acta Sci Vet, 48. https://doi.org/10.22456/1679-9216.105041

9. Duan, H., Yang, S., Zeng, J., Lv, J., Zhang, L., Du, X., ... & Zhao, X. (2023). The effect of melatonin on sheep endometrial epithelial cell apoptosis through the receptor and non-receptor pathways. Gen Comp Endocrinol, 333, 114182. https://doi.org/10.1016/j.ygcen.2022.114182
10. Efe, M., Sarıbay, M. K., Ürer, E. K., & Köse, A. M. (2023). The Effect of Selenium, Vitamin E, Vitamin A and Vitamin D3 Applications on Fertility in Awassi Sheep with Estrus Synchronization During the Breeding Season. Turkish J Agriculture-Food Science and Technology, 11(8), 1331-1336. https://doi.org/10.24925/turjaf.v11i8.1331-1336.5946
11. Enginler SÖ, Dal, G. E., Çetin, A. C., Sabuncu, A., & Baykal, K. (2023). Determination of the effects of oxytocin and carazolol on uterine involution by pulsed-wave Doppler ultrasonography in Kıvırcık ewes. Ankara Üniv Vet Fak Derg, 1-26. https://doi.org/10.33988/auvfd.1025621
12. Fernandes, C. E., Cigerza, C. F., Pinto, G. D. S., Miazi, C., Barbosa-Ferreira, M., & Martins, C. F. (2013). Parturition characteristics and uterine involution in native sheep from Brazilian pantanal. Ciência Animal Brasileira, 14(2), 245-252. https://doi.org/10.5216/cab.v14i2.17926
13. Fernandes, C. E., Cigerza, C. F., Pinto, G. D. S., Miazi, C., Barbosa-Ferreira, M., & Martins, C. F. (2013). Parturition characteristics and uterine involution in native sheep from Brazilian pantanal. Ciência Animal Brasileira, 14(2), 245-252. https://doi.org/ 10.5216/cab.v14i2.17926
14. Fierro, S., Gil, J., Viñoles, C., & Olivera-Muzante, J. (2013). The use of prostaglandins in controlling estrous cycle of the ewe: A review. Theriogenology, 79(3), 399-408. https://doi.org/10.1016/j.theriogenology.2012.10.022
15. Flinn, T., McCarthy, N. L., Swinbourne, A. M., Gatford, K. L., Weaver, A. C., McGrice, H. A., ... & van Wettere, W. H. (2020). Supplementing Merino ewes with melatonin during the last half of pregnancy improves tolerance of prolonged parturition and survival of second-born twin lambs. J Anim Sci, 98(12), skaa372. https://doi.org/10.1093/jas/skaa372
16. González-Candia, A., Veliz, M., Araya, C., Quezada, S., Ebensperger, G., Serón-Ferré, M., ... & Herrera, E. A. (2016). Potential adverse effects of antenatal melatonin as a treatment for intrauterine growth restriction: findings in pregnant sheep. Am J Obstet Gynecol, 215(2), 245-e1. https://doi.org/10.1016/j.ajog.2016.02.040
17. Hauser, B., & Bostedt, H. (2002). Ultrasonographic observations of the uterine regression in the ewe under different obstetrical conditions. J Vet Med A Physiol Pathol Clin Med, 49 (10): 511-516, https://doi.org/ 10.1046/j.1439- 0442.2002.00496.x
18. Hayder, M., & Ali, A (2008). Factors affecting the postpartum uterine involution and luteal function of sheep in the subtropics. Small Rumin Res, 79, 174–178. https://doi.org/10.1016/j.smallrumres.2008.07.023.
19. Kaçar, C., Kamiloğlu, N.N., Uçar, Ö., Arı, U.Ç., Pancarcı, Ş.M., & Güngör, Ö. (2008). İneklerde Beta karoten+ E vitamini uygulamasıyla kombine edilen Ovsynch ve Cosynch senkronizasyon programlarının gebelik oranı üzerine etkisi. Kafkas Üniv Vet Fak Derg, 14(1). https://doi.org/10.9775/kvfd.2008.02-A
20. Kamada, H. (2016). Effects of selenium-rich yeast supplementation on the plasma progesterone levels of postpartum dairy cows. Asian-Australas J Anim Sci, 30(3), 347. https://doi.org/10.5713/ajas.16.0372 Kott, R. W., Thomas, V. M., Hatfield, P. G., Evans, T., & Davis, K. C. (1998). Effects of dietary vitamin E supplementation during late pregnancy on lamb mortality and ewe productivity. J Am Vet Med Assoc, 212(7), 997-1000. https://doi.org/10.2527/jas1983.573553x
21. Köse, M., Kırbaș, M., Dursun, Ș., & Bayrıl, T. (2013). The effect of injections of β-carotene or vitamin E+ Selenium on fertility in ewes in anestrus season. Van Vet J, 24(2), 83-86.
22. Kusakari, N., & Ohara, M. (1997). Effect of melatonin feeding on early onset of reproductive activity in postpartum Suffolk ewes lactating during anestrous season. JRD, 43(1), 97-100. https://doi.org/10.1262/jrd.43.97
23. Lekatz, L. A., Caton, J. S., Taylor, J. B., Reynolds, L. P., Redmer, D. A., & Vonnahme, K. A. (2010). Maternal selenium supplementation and timing of nutrient restriction in pregnant sheep: effects on maternal endocrine status and placental characteristics. J Anim Sci, 88(3), 955-971. https://doi.org/10.2527/jas.2009-2152
24. Li, Y., Liu, Z., Zhang, W., Zhu, C., Chen, X., Zhao, Y., ... & Zeng, W. (2025). Effects of cloprostenol and oxytocin on ultrasonographic changes of uterine horns in postpartum primiparous Hu sheep. Anim Repro Sci, 272, 107651. https://doi.org/10.1016/j.anireprosci.2024.107651
25. Malpaux, B., Viguié, C., Skinner, D. C., Thiéry, J. C., Pelletier, J., & Chemineau, P. (1996). Seasonal breeding in sheep: Mechanism of action of melatonin. Anim Reprod Sci, 42(1-4), 109-117. https://doi.org/10.1016/0378-4320(96)01505-9
26. Mbayahaga, J., Mandiki, S. N. M., Bister, J. L., & Paquay, R. (1998). Body weight, oestrous and ovarian activity in local Burundian ewes and goats after parturition in the dry season. Anim Reprod Sci, 51(4), 289-300. https://doi.org/10.1016/S0378-4320(98)00063-3
27. Medan, M. S., & El-Daek, T. (2015). Uterine involution and progesterone level during the postpartum period in Barbary ewes in north Libya. Open Vet J, 5(1), 18-22. https://doi.org /10.5455/OVJ.2015.v5.i1.p18
28. Milewski, S., Sobiech, P., Błażejak-Grabowska, J., Wójcik, R., Żarczyńska, K., Miciński, J., & Ząbek, K. (2021). The efficacy of a long-acting injectable selenium preparation administered to pregnant ewes and lambs. Animals, 11(4), 1076. https://doi.org/10.3390/ani11041076
29. Mitchell, L. M., Ranilla, M. J., Quintans, G., King, M. E., Gebbie, F. E., & Robinson, J. J. (2003). Effect of diet and GnRH administration on post-partum ovarian cyclicity in autumn-lambing ewes. Anim Reprod Sci, 76(1-2), 67-79. https://doi.org/10.1016/S0378-4320(02)00239-7
30. Morris, S. T. (2017). Overview of sheep production systems. In Advances in sheep welfare. Woodhead Publishing.
31. Noakes, D. E. (2009). The puerperium and the care of the newborn. Veterinary reproduction and obstetrics, 194-205.
32. Nurmala, D. P., Susilorini, T. E., Sjofjan, O., & Adli, D. N. (2024). The effect of selenium as a feed additive on blood parameters antioxidant activity in dairy goat: Meta-analysis. Adv Anim Vet Sci, 12(8), 1588-1595. https://doi.org/10.17582/journal.aavs/2024/12.8.1588.1595
33. O'Shea, J. D., & Wright, P. J. (1984). Involution and regeneration of the endometrium following parturition in the ewe. Cell Tissue Res, 236, 477-485. https://doi.org/10.1007/BF00214253
34. Pollott, G. E., & Gootwine, E. (2004). Reproductive performance and milk production of Assaf sheep in an intensive management system. J Dairy Sci, 87(11), 3690-3703. https://doi.org/10.3168/jds.S0022-0302(04)73508-0
35. Reiter, R. J., Tan, D. X., Manchester, L. C., Paredes, S. D., Mayo, J. C., & Sainz, R. M. (2009). Melatonin and reproduction revisited. Biol Reprod, 81(3), 445-456. https://doi.org/10.1095/biolreprod.108.075655
36. Rubianes, E., & Ungerfeld, R. (1993). Uterine involution and ovarian changes during early post partum in autumn-lambing Corriedale ewes. Theriogenology, 40(2), 365-372. https://doi.org/ 10.1016/0093-691x(93)90274-9
37. Samimi, A. S., Nazifi, S., Asadi, Z., Bakhshaei, F., Farhang, A., Karamizadeh, F., & Aghamiri, S. M. (2023). Effects of vitamin E/selenium and AD3E on progesterone, antioxidant enzymes and pregnancy rate of laparoscopic artificial insemination in sheep. Anim Sci J, 36(138), 77-86. https://doi.org/10.22092/asj.2022.357741.2208
38. Semra, K., Koçak, G., Demir, M. C., & Kaçar, C. (2023). The Effect of Vitamin E-Trace Mineral Treatments on Reproductive Performance in Morkaraman Sheep During the Breeding Season. Kafkas Üniv Vet Fak Derg, 29(2). https://doi.org/10.9775/kvfd.2022.28901
39. Spears, J. W., & Weiss, W. P. (2008). Role of antioxidants and trace elements in health and immunity of transition dairy cows. Vet J, 176(1), 70-76. https://doi.org/10.1016/j.tvjl.2007.12.015
40. Takasaki, A., Tamura, H., Miwa, I., Taketani, T., Shimamura, K., & Sugino, N. (2010). Endometrial growth and uterine blood flow: a pilot study for improving endometrial thickness in the patients with a thin endometrium. Fertil Steril, 93(6), 1851-1858. https://doi.org/10.1016/j.fertnstert.2008.12.062
41. Uematsu, M., Kitahara, G., Sameshima, H., & Osawa, T. (2016). Serum selenium and liposoluble vitamins in Japanese Black cows that had stillborn calves. J Vet Med Sci, 78(9), 1501-1504. https://doi.org/10.1292/jvms.15-0268
42. Vazquez, M. I., Forcada, F., Sosa, C., Casao, A., Sartore, I., Fernández-Foren, A., ... & Abecia, J. A. (2013). Effect of exogenous melatonin on embryo viability and uterine environment in undernourished ewes. Anim Repro Sci, 141(1-2), 52-61. https://doi.org/10.1016/j.anireprosci.2013.07.007
43. Viola, I., Sosa, C., Accornero, P., Manenti, I., Canto, F., Miretti, S., ... & Toschi, P. (2024). Exogenous melatonin ameliorates embryo–maternal cross-talk in early pregnancy in sheep. Reproduction, 168(5). https://doi.org/10.1530/REP-24-0172
44. Vural, M. R., Sel, T., Karagul, H., Ozenc, E., Orman, M., Izgur, H., & Kuplulu, S. (2008). Ultrasonographic examinations of embryonic-fetal growth in pregnant Akkaraman ewes fed selenium supply and dietary selenium restriction. Rev Med Vet, 159(12), 628-633.
45. Wei, M. J., Wang, Z. N., Yang, Y., Zhang, S. J., Tang, H., Li, H., & Bi, C. L. (2022). Selenium attenuates S. aureus-induced inflammation by regulation TLR2 signaling pathway and NLRP3 inflammasome in RAW 264.7 macrophages. Biol Trace Elem Res, 200, 761-767. https://doi.org/10.1007/s12011-021-02676-4
46. Xiao, J., Khan, M. Z., Ma, Y., Alugongo, G. M., Ma, J., Chen, T., ... & Cao, Z. (2021). The antioxidant properties of selenium and vitamin E; their role in periparturient dairy cattle health regulation. Antioxidants, 10(10), 1555. https://doi.org/10.3390/antiox10101555
47. Zanghishe, F., Moeini, M. M., & Hozhabri, F. (2023). Evaluation of the effect of selenium and vitamin E supplementations in late pregnancy on the concentration of some minerals in blood of ewes and their lambs. Anim Prod, 25(2), 155-168. https://doi.org/10.22059/jap.2023.348225.623704
48. Zdunczyk, S., Milewski, S., Baranski, W., Janowski, T., Szczepanski, W., Jurczak, A., & Lesnik, M. (2004). Postpartum uterine involution in primiparous and pluriparous polish longwool sheep monitored by ultrasonography. Bull Vet Inst Pulawy, 48(3), 255-258.
49. Zongling, L., Zhang, W., Chunhao, Z., Xi, C., Yukun, Z., Yanping, W., & Weibin, Z. (2022). Effect of Different Litter Size on The Rate of Postpartum Uterine Involution in Hu Sheep. Kafkas Üniv Vet Fak Der, 28(2). https://doi.org /10.9775/kvfd.2021.26965