Effects of Lauric Acid Supplementation in Milk on Performance and Some Blood Parameters of Newborn Calves

Öz

This study examines the prebiotic benefits of lauric acid in the diets of newborn calves. Thirty female Holstein calves were divided into three groups: The Control (C) group received bulk tank milk; the Positive Control (PC) group was given soybean oil mixed with milk; and the Trial (T) group was given milk supplemented with lauric acid. The groups were fed the same basal calf starter feed. The trial concluded on the 60th day of the weaning period. The study monitored calf performance and blood metabolic markers on days 3, 7, 15, 30, 45, and 60 after birth. Lauric acid supplementation did not significantly influence calves' initial feed intake, body weight gain, or blood parameters. Although the incidence of diseases in the lauric acid group was not statistically significant, it was numerically lower than in the other groups and was comparable to them (number of cases; 3, 6, 6; T, PC, C, respectively). Furthermore, a comparison was made between all animals affected by the disorders and those who had never encountered any health issues in relation to the performance and blood parameters. The blood glucose level of the calf recovered from illness (CRI) was markedly lower (p<0.001), whereas Triglyceride (p<0.001), Total cholesterol (p<0.1), Haptoglobin (p<0.001), and Serum amyloid A (p<0.001) levels were considerably elevated. The feed intake and live weight gains of sick calves were markedly lower than those of healthy calves (p<0.001). As a result, supplementing calves’ milk with lauric acid reduced disease incidence but had no significant effect on performance or metabolism.

Anahtar Kelimeler

• Calf
• Health
• lauric acid
• Metabolism
• Performance

Yenidoğan Buzağılarda İçme Sütüne İlave Edilen Laurik Asitin Performans ve Bazı Kan Parametreleri Üzerine Etkileri

Öz

Bu çalışma, yeni doğan buzağıların rasyonlarındaki laurik asidin prebiyotik faydalarını incelemek amacıyla gerçekleştirilmiştir. Otuz dişi Holstein buzağı üç gruba ayrıldı: Kontrol grubuna tank sütü, Pozitif Kontrol grubuna süt ile karıştırılmış soya fasulyesi yağı ve deneme grubuna laurik asit ilave edilmiş süt verildi. Tüm gruplar standart buzağı başlangıç yemi ile beslendi. Deneme, sütten kesimin 60. gününde sona erdi. Çalışmada buzağı performansı ve doğumdan sonraki 3, 7, 15, 30, 45 ve 60. günlerde kanda metabolik parametreleri incelendi. Laurik asit ilavesi buzağı başlangıç yemi tüketimi, canlı ağırlık artışı, hastalık insidensi ve kan parametrelerine istatiksel olarak anlamlı düzeyde etki etmemiştir. Ancak laurik asit verilen grupta hastalık insidensi istatiksel olarak anlamlı olmasa da rakamsal olarak diğer gruplardan az olup onların yarısı kadardır (vaka sayıları U, PK, K gruplarında sırasıyla; 3, 6, 6’dır). Ayrıca çalışmada ayırt etmeksizin hastalık geçirmiş tüm hayvanlar ile sağlık açısından hiç problem yaşamamış hayvanlarda gerek kan parametreleri gerekse performans yönünden karşılaştırılmıştır. Hastalık geçirmiş buzağıların (HGB) kanda glukoz seviyesi önemli düzeyde düşük (p< 0,001); Trigliserit, Total kolesterol, Haptoglobin ve Serum amiloid A seviyeleri ise önemli düzeyde yüksek (p<0,001) bulunmuştur. Ayrıca hastalık geçiren buzağıların yem tüketimleri ve canlı ağırlık artışları sağlıklı buzağılardan önemli düzeyde düşük (p<0,001) bulunmuştur. Sonuç olarak, süte ilaveten laurik asitin buzağılara içirilmesinin performans ve metabolizma parametreleri üzerine önemli bir etkisi olmamasına rağmen hastalık insidensini düşürdüğü kanısına varılmıştır.

Anahtar Kelimeler

• Buazğı
• Laurik acid
• Metabolizma
• Performans
• Sağlık

Kaynakça

1. Abd-Elrahman, A. H. (2011). Colibacillosis in newly born buffalo calves and role of Lacteol Fort in preventing recurrence of calf diarrhea. Life Science Journal, 8, 497–502.
2. Albayrak, H., & Kabu, M. (2016). Determining serum hap toglobin and cytokine concentrations in diarrheic calves. Fırat Universitesi Sağlık Bilimleri Dergisi, 30, 113-117.
3. AOAC. (1990). Official Methods of Analysis. 16th. Association of Official Analytical Chemists, Washington, D.C. Bach, A. C., & Babayan, V. K. (1982). Medium-chain triglycerides: an update. The American Journal of Clinical Nutrition, 36(5), 950-962.
4. Bendali, F., Bichet, H., Schelcher, F., & Sanaa, M. (1999). Pattern of diarrhoea in newborn beef calves in south-west France. Veterinary Research, 30, 61–74.
5. Bichalho, R.C., Galvao, K.N., & Cheong, S.H. (2007). Effect of stillbirths on dam survival and reproduction performance in Holstein dairy cows. Journal of Dairy Science, 90, 2797–803. htpps://doi.org/10.3168/jds.2006-504
6. Bozukluhan, K., Merhan, O., Gokce, H. I., Deveci, H. A., Gokce, G., Ogun, M., & Marasli, S. (2017). Alterations in lipid profile in neonatal calves affected by diarrhea.Veterinary world, 10(7), 786. https://doi.org/10.14202/vetworld.2017.786-789
7. Conato, M., & Sumera, F. (2012). Biodegradable polyesters and polyamides from difunctionalized lauric and coconut fatty acids. Journal of Polymers and the Environment, 20(1), 217-223.
8. Dayrit, F. M. (2015). The properties of lauric acid and their significance in coconut oil. Journal of the American Oil Chemists' Society, 92(1), 1-15.

9. Donovan, G. A., Dohoo, I. R., Montgomery, D. M., & Bennett, F. L. (1998). Associations between passive immunity and morbidity and mortality in dairy heifers in Florida, USA. Preventive Veterinary Medicine, 34, 31–46. https://doi.org/10.1016/s0167-5877(97)00060-3
10. Eckersall, P., & Conner, J. (1988). Bovine and canine acute phase proteins. Veterinary Rerearch Communications, 12, 169–178.
11. Feingold, K. R., Pollock, A. S., Moser, A. H., Shigenaga, J. K. & Grunfeld, C. (1995). Discordant regulation of proteins of cholesterol metabolism during the acute phase response. Journal of Lipids Research, 36, 1474-1482.
12. Graulet, B., Gruffat-Mouty, D., Durand, D., & Bauchart, D. (2000). Effects of milk diets containing beef tallow or coconut oil on the fatty acid metabolism of liver slices from preruminant calves. British Journal of Nutrition, 84, 309-318.
13. Gunstone, F. D. (1996). Fatty acid and lipid chemistry. Springer.
14. Hajimohammadi, A., Nazifi, S., Ansari-Lari, M., Khoshmanzar, M. R., & Bigdeli, S. M. (2013). Identifying relationships among acute phase proteins (haptoglobinhaptoglobinhaptoglobin, serum amyloid A, fibrinogen, ceruloplasmin) and clinical findings in dairy calf diarrhea. Comparative Clinical Pathology, 22, 227-232.
15. Horadagoda, N., Knox, K., Gibbs, H., Reid, S., Horadagoda, A., Edwards, S., & Eckersall, P. (1999). Acute phase proteins in cattle: discrimination between acute and chronic inflammation. Veterinary Record, 144, 437.
16. Hu, F., Piao, M., Yang, C., Diao, Q., & Tu, Y. (2023). Effects of coconut oil and palm oil on growth, rumen microbiota, and fatty acid profile of suckling calves. Microorganisms, 11, 655. https://doi.org/10.3390/microorganisms11030655
17. Kertz, A. F., Prewitt, L. R., & Everett Jr, P. (1979). An early weaning calf program: summarization and review. Journal of Dairy Science, 62, 1835-1843. https://doi.org/10.3168/jds.S0022-0302(79)83508-0
18. Lorenz, I., Earley, B., Gilmore, J., Hogan, I., Kennedy, E., & More, S. J. (2011). Calf health from birth to weaning. III. Housing and management of calf pneumonia. Irish Veterinary Journal, 64, 1-9. https://doi.org/10.1186/2046-0481-64-14
19. Ly, H., Francone, O. L., Fielding, C. J., Shigenaga, J. K., Moser, A. H., Grunfeld, C., & Feingold, K. R. (1995). Endotoxin and TNF lead to reduced plasma LCAT activity and decreased hepatic LCAT mRNA levels in Syrian hamsters. Journal of Lipids Research, 36, 1254-1263.
20. Mee, J. F. (2008). Newborn dairy calf management. Veterinary Clinics of North America: Food Animal Practice, 24(1), 1-17.
21. Meier, U. (2006). A note on the power of Fisher's least significant difference procedure. Pharmaceutical statistics, 5(4), 253–263. https://doi.org/10.1002/pst.210
22. Mensink, R. P., Zock, P. L., Kester, A. D., & Katan, M. B. (2003). Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. The American Journal of Clinical Nutrition, 77(5), 1146-1155. https://doi.org/10.1093/ajcn/77.5.1146
23. Myers, D. (2006). Surfactant Science and Technology. John Wiley & Sons.
24. National Animal Health Monitoring System (NAHMS, US). (2010). Dairy 2007: Heifer Calf Health and Management Practices on US Dairy Operations, 2007. US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Animal Health Monitoring System. National Research Council (NRC). (2001). Nutrient Requirements of Dairy Cattle: Seventh Revised Edition. Washington, DC: The National Academies Press. https://doi.org/10.17226/9825.
25. Nevin, K.G., & Rajamohan, T. (2004). Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clinical Biochemistry, 37(9), 830-835.
26. Ogoshi, T., & Miyawaki, Y. (1985). Soap and related products: Palm and lauric oil. Journal of the American Oil Chemists’ Society, 62(2), 331-335.
27. Rocha Valdez, J., Gonzalez-Avalos, R., Avila-Cisneros, R., Peña-Revuelta, B., & Reyes-Romero, A. (2019). Economic impact of mortality and morbidity from diseases in dairy calves. Abanico veterinario, 9, 1-7. https://doi.org/10.21929/abavet2019.920
28. Shilling, M., Matt, L., Rubin, E., Visitacion, M. P., Haller, N. A., & Johns, N. P. (2013). Antimicrobial effects of virgin coconut oil and its medium-chain fatty acids on Clostridium difficile. Journal of Medicinal Food, 16(12), 1079-1085.
29. Spicer, H.M., Goonewardene, L.A., & McNeill, A.O, (1994). Alberta dairy farm survey response. Journal of Dairy Science 1994, 77, 3460–72. https://doi.org/10.3168/jds.S0022-0302(94)77289-1
30. Toullec, R., & Guilloteau, P. (1989). Research into the digestive physiology of the milk-fed calf. Van Soest, P. V., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of dairy science, 74(10), 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
31. Wittum, T., Young, C., Stanker, L., Griffin, D., Perino, L., & Littledike, E. (1996). HaptoglobinHaptoglobin response to clinical respiratory tract disease in fee dlot cattle. American Journal of Veterinary Research, 57, 646.
32. Uyarlar, C., Rahman, A., Ozcinar, U., Cetingul, İ. S., Gultepe, E. E., & Bayram, I. (2024). Effect of Myrtus communis L. Plant Extract as a Milk Supplement on the Performance, Selected Blood Parameters and Immune Response of Holstein Calves. Animals, 14(5), 725. https://doi.org/10.3390/ani14050725
33. Yamamoto, Y., Morikawa, T., Kawai, T., & Nonomura, Y. (2017). Selective bactericidal activity of divalent metal salts of lauric acid. Acs Omega, 2(1), 113-121. https://doi.org/10.1021/acsomega.6b00