Ratlarda Gentamisin Kaynaklı Böbrek Toksisitesi Üzerine Polidatin’ in Etkilerinin Araştırılması

Öz

Bir aminoglikozid antibiyotik olan gentamisin (GNT), tübüler apoptoz ve inflamasyon gibi mekanizmalar yoluyla nefrotoksisiteye neden olur. Antioksidan ve anti-inflamatuar özelliklere sahip doğal bir polifenolik bileşik olan polidatin (Poly), böbrek hasarını hafifletme potansiyeli göstermiştir. Bu çalışma, biyokimyasal, moleküler ve histopatolojik yöntemler kullanarak GNT kaynaklı böbrek hasarı olan sıçanlarda Poly'nin koruyucu etkilerini araştırmayı amaçlamıştır. 35 Wistar albino sıçan, kontrol, Poly (100 mg/kg), GNT (100 mg/kg) ve iki kombine tedavi grubu (GNT+Poly 50 mg/kg ve 100 mg/kg) olmak üzere 5 gruba (grup başına 7 sıçan) ayrıldı. 7 günlük tedaviden sonra böbrek fonksiyon belirteçleri, oksidan-antioksidan parametreler, gen ekspresyonu (NF-κB, TNF-α, Kaspaz-3, Bax, Bcl-2, KIM1, AQP2) ve histopatolojik değerlendirme analizi için böbrek dokuları ve kan toplandı. GNT serum üre ve kreatinin düzeylerini (p<0,001), MDA düzeylerini (p<0,001) arttırdı ve antioksidanları (p<0,001) azalttı; ayrıca NF-κB ve TNF-α ekspresyonunu artırdı (p<0,001), Kaspaz-3 ve Bax'ı artırdı (p<0,001) ve Bcl-2 düzeylerini azalttı (p<0,001). GNT ile birlikte uygulandığında Poly, MDA düzeylerini düşürdü (p<0,001) ve GSH düzeylerini artırdı (p<0,001), inflamasyon belirteçlerini (NF-κB ve TNF-α) azalttı (p<0,01), Kaspaz-3 ve Bax'ı azalttı (p<0,01) ve Bcl-2 düzeylerini artırdı (p<0,01) ve ayrıca histolojik hasarı iyileştirdi ve histolojik skoru azalttı (p<0,05). GNT kaynaklı böbrek toksisitesinde, Poly 100 tedavisi oksidatif stres, inflamasyon ve apoptozu tersine çevirerek böbrek koruması sağlamıştır.

Anahtar Kelimeler

• Apoptozis
• gentamisin
• nefrotoksisite
• oksidatif stres
• polidatin

Investigation of the Effects of Polydatin on Gentamicin-Induced Renal Toxicity in Rats

Öz

Gentamicin (GNT), an aminoglycoside antibiotic, induces nephrotoxicity through mechanisms like tubular apoptosis and inflammation. Polydatin (Poly), a natural polyphenolic compound with antioxidant and anti-inflammatory properties, has shown potential in alleviating renal damage. This study aimed to investigate the protective effects of Poly in rats with GNT-induced kidney injury using biochemical, molecular, and histopathological methods. 35 Wistar albino rats were divided into 5 groups (7 rats/group), including control, Poly (100 mg/kg), GNT (100 mg/kg), and two combined treatment groups (GNT+Poly at 50 mg/kg and 100 mg/kg). After 7 days of treatment, kidney tissues and blood were collected for analysis of renal function markers, oxidant-antioxidant parameters, gene expression (NF-κB, TNF-α, Caspase-3, Bax, Bcl-2, KIM1, AQP2), and histopathological evaluation. GNT increased serum urea and creatinine levels (p<0.001), increased MDA levels (p<0.001) and decreased antioxidants (p<0.001); also increased the expression of NF-κB and TNF-α (p<0.001), increased Caspase-3 and Bax (p<0.001) and decreased Bcl-2 levels (p<0.001). When administered together with GNT, Poly decreased MDA levels (p<0.001) and increased GSH levels (p<0.001), decreased inflammation markers (NF-κB and TNF-α) (p<0.01), decreased Caspase-3 and Bax (p<0.01) and increased Bcl-2 levels (p<0.01), and also improved histological damage and decreased histological score (p<0.05). In GNT-induced renal toxicity, Poly 100 treatment provided renal protection by reversing oxidative stress, inflammation, and apoptosis.

Anahtar Kelimeler

• Apoptosis
• gentamicin
• nephrotoxicity
• oxidative stress
• polydatin

Kaynakça

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