Effect of Chemical Castration Using High Osmolarity Solutions on Spermatological Parameters in Rats

Öz

In our study, the changes in spermatological parameters in rats subjected to chemical castration with high osmolarity solutions were investigated. In the study, they were divided into 8 groups, with 8 rats in each group, and a total of 64 Wistar albino male rats were used. No chemicals were applied to the rats in the control group. Rats in the second group were sterilized surgically. 0.9% physiological saline was administered to the 3rd group, 0.9% physiological saline and 10% calcium chloride to the 4th group, 20% mannitol to the 5th group, 20% mannitol and 10% calcium chloride to the 6th group, 20% dextrose to the 7th group, 20% dextrose and 10% calcium chloride solutions were administered intratesticularly to the 8th group at a dose of 0.1 ml/100gr. Andrological findings compared to the control group; it was determined that there was a significant difference in sperm motility and sperm density (p<0.001) in groups 3, 5, and 7. However, it was observed that there was no difference in the rate of abnormal spermatozoon. In groups 2, 4, 6, and 8, motility, density, abnormal sperm count and epididymis weights could not be measured because sperm cells could not be collected. In addition, it was determined that the weights of the epididymis and right cauda epididymis in groups 3, 5, 7 decreased compared to the control (p<0.05), while in groups 2, 4, 6, 8, measurements could not be made because tissue samples could not be taken, and the weights of testicles, vesicle seminalis and prostate decreased significantly (p<0.001) compared to the control. In conclusion; it has been concluded that giving high osmolarity solutions in combination with calcium chloride will be much more effective in the intratesticular chemical sterilization process.

Anahtar Kelimeler

• Chemical castration
• spermatological parameters
• rat
• calcium chloride

Ratlarda Yüksek Osmolariteli Solüsyonlar Kullanılarak Yapılan Kimyasal Kastrasyonun Spermatolojik Parametrelere Etkisi

Öz

Çalışmamızda, yüksek osmolariteli solüsyonlarla kimyasal kısırlaştırma oluşturulan ratlarda spermatolojik parametrelerin değişimi araştırılmıştır. Çalışmada, her grupta 8 rat kullanılarak 8 gruba ayrıldı ve toplam 64 Wistar albino erkek rat kullanıldı. Kontrol grubundaki ratlara herhangi bir kimyasal uygulanmadı. 2. gruptaki ratlar cerrahi olarak kısırlaştırıldı. 3. gruba % 0.9’luk serum fizyolojik, 4. gruba %0.9’luk serum fizyolojik ile %10 kalsiyum klorür, 5. gruba %20 mannitol, 6. gruba %20 mannitol ile %10 kalsiyum klorür, 7. gruba %20 dekstroz, 8. gruba %20 dekstroz ile %10 kalsiyum klorür solüsyonları 0.1ml/100 gr dozda intratestiküler uygulandı. Androlojik bulgular kontrol grubuyla kıyaslandığında; 3, 5, 7. gruplarda sperm motilitesi ve sperma yoğunluğunda (p<0,001) önemli derecede farklılık olduğu belirlendi. Ancak anormal spermatozoon oranında fark olmadığı görüldü. 2, 4, 6, 8. gruplarında ise sperm hücresi alınamadığından motilite, yoğunluk, anormal sperm sayısı ve epididimis ağırlıkları ölçümü yapılamadı. Ayrıca 3, 5, 7 gruplarında epididimis ve sağ kauda epididimis ağırlıklarının kontrole göre (p<0,05) düştüğü, 2, 4, 6, 8 gruplarında ise doku örneği alınamadığından ölçüm yapılamadığı ve testis, vezikula seminalis, prostat ağırlıklarının ise kontrole göre önemli derecede (p<0,001) düştüğü belirlendi. Sonuç olarak; testis içi yapılan kimyasal kısırlaştırma işleminde, yüksek osmolariteli solüsyonların kalsiyum klorür ile kombine olarak verilmesinin çok daha etkili olacağı kanaatine varılmıştır.

Anahtar Kelimeler

• Kimyasal kastrasyon
• spermatolojik parametreler
• rat
• kalsiyum klorür

Kaynakça

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