Yeni Zelanda Tavşanlarında Bazı Ekstremite Kemiklerinin 3 Boyutlu Tarayıcı ve Bilgisayarlı Tomografi İle 3 Boyutlu Rekonstrüksiyonu: Morfolojik İnceleme

Öz

Son yıllarda, anatomi alanında 3 Boyutlu (3B) tarayıcılar ve bilgisayarlı tomografi kullanan çalışmaların sayısında belirgin bir artış olmuştur. Ancak literatürde, bu iki yöntem arasındaki uyumluluğu değerlendiren çalışmaların sınırlı olduğu gözlenmiştir. Yapılan çalışmada toplam 12 yetişkin tavşan kullanılmıştır. Maserasyonun ardından, seçilen ekstremite kemikleri hem bilgisayarlı tomografi (BT) hem de 3B tarayıcı kullanılarak 3B rekonstrüksiyona tabi tutulmuştur. Elde edilen modeller, morfolojik olarak analiz edilmiştir. Scapula’da, acromion’un ventral ucunun proc. hamatus'ta sonlandığı ve metacromion olarak caudal olarak uzandığı gözlemlendi. Humerus’ta, tuberculum majus’un caput humeri'yi hafifçe aştığı ve distal uçta foramen supratrochleare gözlendi. Önkol iskeleti radius ve ulna’dan oluştuğu ve ulna’nın daha gelişmiş olduğu gözlendi. Radius, belirgin bir fovea capitis radii ve proc. styloideus radii sahipken, ulnae’da belirgin bir tuber olecrani ve processus styloides ulnae varlığı tespit edildi. Femur’da, trochanter major’un caput femoris düzeyini aştığı ve crista intertrochanterica trochanter major ile trochanter minor arasında yer aldığı gözlendi. Distal femurda, facies poplitea belirgindi. Ossa cruris, tek bir yapı halinde birleşmiş tibia ve fibula şeklinde olduğu tespit edildi. Sonuç olarak, her iki yöntemle oluşturulan 3B modellerde belirlenen anatomik noktalar benzerlik göstermekteydi. CT, tüm dokuları tarama ve hızlı veri sağlama konusunda üstünlük sağlarken, 3B tarayıcılar taşınabilirlik, renkli görüntüleme ve maliyet açısından avantajlar sunmaktadır. Yöntemlerin birlikte kullanılması, veteriner anatomi eğitimi için faydalı olabileceği düşünülmektedir.

Anahtar Kelimeler

• 3B rekonstrüksiyon
• 3B tarayıcı
• Bilgisayarlı tomografi
• Metot karşılaştırma

3D Reconstruction of Some Limb Bones in New Zealand Rabbits with 3D Scanning and Computed Tomography: Morphological Investigation

Öz

In recent years, there has been a notable increase in the number of studies utilizing three-dimensional (3D) scanners and computed tomography in the field of anatomy. However, the literature reveals that studies evaluating the compatibility between these two methods remain limited. In this study, a total of 12 adult rabbits were used. Following maceration, selected limb bones were subjected to 3D reconstruction using both computed tomography (CT) and 3D scanner. The resulting models were analyzed morphologically. On the scapula, the ventral end of the acromion was observed to terminate at the proc. hamatus and extend caudally as the metacromion. In the humerus, the tuberculum majus slightly surpassed the caput humeri, and a foramen supratrochleare was present at the distal end. The antebrachial skeleton consisted of the radius and ulna, with the ulna being more developed. The radius displayed a distinct fovea capitis radii and processus styloideus radii, while the ulna exhibited a prominent tuber olecrani and a proc. styloideus ulnae. The femur, the trochanter major extended beyond the femoral head, with the crista intertrochanterica located between the trochanter major and trochanter minor. At the distal femur, facies poplitea were clearly visible. The crural skeleton consisted of the tibia and fibula, which were fused into a single structure. In conclusion, the anatomical landmarks identified in the 3D models generated by both methods were consistent. While CT excels in scanning entire tissues and providing rapid data, 3D scanners offer advantages portability, color imaging, and cost. Combined use of methods is considered beneficial for veterinary anatomy education.

Anahtar Kelimeler

• 3D scanner
• 3D reconstruction
• Computed tomography
• Method comparison

Kaynakça

1. Akgün, R.O., Orhan, İ. Ö., & Okan, E. (2021). Three-dimensional bone modeling of forelimb joints in New Zealand Rabbit: A Micro-Computed Tomography study. Ankara Universitesi Veteriner Fakultesi Dergisi, 68, 4, 355-63. https://doi.org/10.33988/auvfd.762615.
2. Al-Ubaidy, A. A. H., Jabbar, A. I., & Al-Agele, R. A. A. (2020). Morphometric comparative study of scapula as example of shoulder girdle and os coxa as example of pelvic girdle in hare and domesticated rabbit. EurAsian Journal of BioSciences, 14, 5093-5. e-ISSN: 1307-9867.
3. Argot, C. (2001). Functional-adaptive anatomy of the forelimb in the Didelphidae, and the paleobiology of the Paleocene marsupials Mayulestes ferox and Pucadelphys andinus. Journal of Morphology, 247, 1, 51-79. https://doi.org/10.1002/1097-4687(200101)247:1<51::AID-JMOR1003>3.0.CO;2-%23.
4. Bakici, C., Akgun, R., Ekim, O., Batur, B., Bakici., M., Ozen., D., & Soydal., C. (2021). Three dimensional modeling and quantitative analysis of long bone parameters of rabbit using micro-computed tomography. Iranian Journal of Veterinary Research, 22, 2, 140. https://doi.org/10.22099/ijvr.2021.39092.5688.
5. Barone, R. (1986). Anatomie comparée des mammifères domestiques. Tome 1: Ostéologie, Paris, Vigot Freres, p. 33-733. ISBN-13: 978-2711491605.
6. Chiarello, G. P., Gomes, S. P., Sasahara, T. H. D. C, & Miglino, M. A. (2021). Anatomical characteristics of the bones of the thoracic limb of white-eared opossum (Didelphis albiventris). International Journal of Morphology, 39(2), 416-422. https://doi.org/10.4067/S0717-95022021000200416.
7. Chin, Jr, E. (1957). The rabbit: an illustrated anatomical guide. Master’s Thesis, College of the Pacific. Çevik-Demirkan, A., Özdemir, V., Türkmenoğlu, İ., & Demirkan, I. (2007). Anatomy of the hind limb skeleton of the chinchilla (Chinchilla lanigera). Acta Veterinaria Brno, 76, 4, 501-7. https://doi.org/10.2754/avb200776040501.
8. da Silveira, E. E., da Silva, Lisboa, Neto, A. F., Carlos, Sabino, Pereira, H., Ferreira, J. S., Dos Santos, A. C., Siviero, F., & de Assis Neto, A.C. (2021). Canine skull digitalization and three-dimensional printing as an educational tool for anatomical study. Journal Of Veterinary Medical Education, 48, 6, 649-55. https://doi.org/10.3138/jvme-2019-0132.

9. de Araújo, F., Sesoko, N., Rahal, S. C., Teixeira, C. R., Müller T. R., & Machado M. R. F. (2013). Bone morphology of the hind limbs in two caviomorph rodents. Anatomia, Histologia, Embryologia, 42, 2, 114-23. https://doi.org/10.1111/j.1439-0264.2012.01172.x.
10. Doubell, N. S., Sahd, L., & Kotzé, S. H. (2020). Comparative forelimb morphology of scratch‐digging and chisel‐tooth digging African mole‐rat species. Journal of Morphology, 281, 9, 1029-46. https://doi.org/10.1002/jmor.21229.
11. Edl, M., Mizerák, M., & Trojan, J. (2018). 3D laser scanners: History and applications. Acta Simulatio, 4, 4, 1-5. https://doi.org/10.22306/asim.v4i4.54.
12. El-Ghazali, H., & El-Behery, E., (2018). Comparative macro-anatomical observations of the appendicular skeleton of New Zealand rabbit (Oryctolagus cuniculus) and domestic cat (Felis domestica) thoracic limb. International Journal of Veterinary Science, 7, 3, 127-33. https://www.ijvets.com/pdf-files/Volume-7-no-3-2018/127-133.pdf.
13. Girgiri, I. A., Yahaya, A., Gambo, B. G., Majama, Y. B., & Sule A. (2016). Osteomorphology of the appendicular skeleton of four-toed african hedgehogs (Atelerix albiventris) Part (2): Pelvic limb. Global Veterinaria, 16, 413-8. https://doi.org/10.5829/idosi.gv.2016.16.05.10352.
14. Gökmen, S., Pehlivan, A., Aksoy, A. (2019). Laboratuvar Hayvanlarında Ötenazi Yöntemleri. Etlik Veteriner Mikrobiyoloji Dergisi, 30, 1, 87-94. https://doi.org/10.35864/evmd.586565.
15. Hackmann, C. H., Dos, Reis, D. A., de-Assis-Neto, A. C. (2019). Digital revolution in veterinary anatomy: confection of anatomical models of canine stomach by scanning and three-dimensional printing (3D). International Journal of Morphology, 37, 2, 486-90. https://doi.org/10.4067/S0717-95022019000200486.
16. Hussein, Al-Ubaidy, A. A., Jabbar, A. I., Abood, Al-Agele, R. A. (2020). Morphometrie comparative study of scapula as example of shoulder girdle and os coxa as example of pelvic girdle in hare and domesticated rabbit. EurAsian Journal of BioSciences, 14, 2, 5093-95. ISSN: 1307-9867.
17. Kazeem, E. O., Oyewole, O. J., Ifukot, U. L. (2020). Gross anatomical features and osteometric variables of the scapula of the african tree squirrel (Funisciurus anerythrus), house (Rattus Rattus) and wistar rats. Anatomy Journal of Africa, 9, 2, 1773-81. https://doi.org/10.4314/aja.v9i2.198923.
18. Lipman, N., Marini, R., Erdman, S. (1990). A comparison of ketamine/xylazine and ketamine/xylazine/acepromazine anesthesia in the rabbit. Laboratory animal science, 40, 4, 395-8. PMID: 2166867.
19. Montoya-Sanhueza, G., Šaffa, G., Šumbera, R., Chinsamy, A., Jarvis, J.U., Bennett, N., C. (2022). Fossorial adaptations in African mole-rats (Bathyergidae) and the unique appendicular phenotype of naked mole-rats. Communications Biology, 5, 1, 1-13.
20. Mould, R. (1995). Röntgen and the discovery of X-rays. The British journal of radiology, 68, 815, 1145-76. https://doi.org/10.1259/0007-1285-68-815-1145.
21. Oguntoye, C., Oke, B. (2014). A comparison of xylazine/ketamine, diazepam/ketamine and acepromazine/ketamine anaesthesia in rabbit. Sokoto Journal of Veterinary Sciences, 12, 3, 21-5. https://doi.org/10.4314/sokjvs.v12i3.4.
22. Olude, M. A., Olopade, J. O., Akinloye, A. K., Mustapha, O. A. (2010). Macro-anatomical investigations of the skeletons of the African giant rat (Cricetomys gambianus Waterhouse 1840) II: Fore limb. Eur J Anat, 14, 1, 19-23. ISSN: 2340-311X.
23. Olude, M.A, Olopade, J.O., Mustapha, O.A. (2023). Macro-anatomical investigations of the skeletons of the African giant rat (Cricetomys gambianus Waterhouse): Pelvic limb. European Journal of Anatomy, 13, 3, 127-31.
24. Özkan, Z. E. (2002a). Macro-anatomical investigations on the forelimb skeleton of mole-rat (Spalax leucodon Nordmann). Vet. arhiv 72, 91-99, 2002.
25. Özkan, Z. E. (2002b). Macro-anatomical investigations on the hind limb skeleton of mole-rat (Spalax leucodon Nordmann). Veterinarski Arhiv, 72(3), 159-166.
26. Özkan, Z. E. (2002c). Macro-anatomical investigations on the skeletons of hedgehog (Erinaceus europaeus L.). II. Ossa membri pelvini. Veterinarski arhiv, 72, 4, 213-20. ISSN: 0372-5480.
27. Palanisamy, D., Tomar, M., Ankem, P. B., Sekhar, R. (2020). Humerus of Indian Wildcat (Felis silvestris ornata: Gray, 1830)—A Gross Osteological Study. International Journal of Livestock Research, 10, 49-54. http://dx.doi.org/10.5455/ijlr.20200211041708.
28. Polly, P. D., Hall, B. (2007). Limbs in mammalian evolution. Fins into limbs: evolution, development and transformation, 245-68. ISBN: 9780226319942.
29. Raja, V., Fernandes, K. J. (2007). Reverse engineering: an industrial perspective, Springer Science & Business Media, p. 1-115. http://dx.doi.org/10.1007/978-1-84628-856-2.
30. Remondino, F. (2011). Heritage recording and 3D modeling with photogrammetry and 3D scanning. Remote sensing, 3, 6, 1104-38. http://dx.doi.org/10.3390/rs3061104.
31. Rosicky, J., Grygar, A., Chapcak, P., Bouma, T., et al. (2016). Application of 3D scanning in prosthetic & orthotic clinical practice. Proceedings of the 7th international conference on 3D body scanning technologies, 88-97. http://dx.doi.org/10.15221/16.088.
32. Saber, A. S. M. (2013). Some morphological observations on the thoracic limb bones of the Hairy-Nosed Wombat (Lasiorhinus latifornis, Owen). Journal of Veterinary Anatomy, 6, 2, 93-109. http://dx.doi.org/10.21608/jva.2013.45026.
33. Sahd, L., Bennett, N. C., Kotzé, S. (2019). Hind foot drumming: morphological adaptations of the muscles and bones of the hind limb in three African mole‐rat species. J Anat, 235, 4, 811-24. http://dx.doi.org/10.1111/joa.13028.
34. Salami, S.O., Onwuama, K.T., Maidawa, M.S., Imam, J., Ojo, S.A. (2011). Morphological studies of the appendicular skeleton of the African giant pouched rat (Cricetomys gambianus) Part (1) pectoral limb. Journal of veterinary medicine and animal health, 3, 7, 82-7.
35. Schmalz, C., Forster, F., Schick, A., Angelopoulou, E. (2012). An endoscopic 3D scanner based on structured light. Medical image analysis, 16, 5, 1063-72. http://dx.doi.org/10.1016/j.media.2012.04.001.
36. Seckel, L., Janis, C. (2008). Convergences in scapula morphology among small cursorial mammals: an osteological correlate for locomotory specialization. Journal of Mammalian Evolution, 15, 4, 261-79.
37. Shunmugam, R., Sundaram, M. (2022). Comparative Morphology of the Humerus of Rabbit, Guinea Pig and Mongoose. Indian J Vet Sci Biotechnol, 18, 4, 59-63. e- ISSN: 2395-1176.
38. Sundaram, V., Dumas, N., Adogwa, A., Rao, S., Nayak, S.B., (2015). Morphological studies of the forelimb skeleton of the orange rumped agouti (Dasyprocta leporina Linnaeus, 1758). Annual Research & Review in Biology, 1-9.
39. Şeicaru, A. (2019). Biodiversity of the thoracic limb skeleton in Coypu. Scientific Works Series C. Veterinary Medicine, LXV, 2, 11-6.
40. Taşbaş, M. (1965). Maserasyon tekniği üzerinde araştırmalar. Ankara Univ Vet Fak Derg, 12(04), 325-330. e-ISSN: 1308-2817.
41. Van Staden, S. L. (2014). The thoracic limb of the suricate (Suricata suricatta): osteology, radiologic anatomy, and functional morphologic changes. J Zoo Wildl Med, 45, 3, 476-86. http://dx.doi.org/10.1638/2012-0280R1.1.
42. Wang, H-H., Wang, Y-X., J., Sheng, H., Zhang, G., Qin, L., Ahuja, A.T., Teng, L-S. (2009). Fossa trochanterica of the proximal femur in rabbits: an anatomic structure for potential misinterpretation on magnetic resonance images. Acta Radiologica, 50, 2, 212-6.
43. Wilhite, R., Wölfel, I. (2019). 3D Printing for veterinary anatomy: An overview. Anat Histol Embryol, 48, 6, 609-20. http://dx.doi.org/10.1111/ahe.12502.
44. Witkowska, A., Alibhai, A., Hughes, C., Price, J., Klisch, K., Sturrock, C. J., Rutlandet C. S. (2014). Computed tomography analysis of guinea pig bone: architecture, bone thickness and dimensions throughout development. PeerJ, 2, e615. http://dx.doi.org/10.7717/peerj.615.
45. Yılmaz, O., Soygüder, Z., Yavuz, A. (2020a). Three-Dimensional Investigation by Computed Tomography of the Clavicle and Scapula in Van Cats. Van Vet J, 31, 1. http://dx.doi.org/10.36483/vanvetj.644080.
46. Yılmaz, O., Soygüder, Z., Yavuz, A. (2020b). Van Kedilerinde Humerus ve Antebrachium’un Bilgisayarlı Tomografi ile Anatomik, Morfometrik ve Volümetrik Olarak İncelenmesi. Harran Univ Vet Fak Derg, 9, 2, 161-9. http://dx.doi.org/10.31196/huvfd.792943.
47. Yılmaz, S., Özkan, Z. E., Özdemir, D. (1998). Oklu Kirpi (Hystrix cristata) iskelet sistemi üzerinde makro-anatomik araştırmalar. I. Ossa membri thoracici. Turk J Vet Anim Sci, 22, 4, 389-92. ISSN: 1300-0128.