Boer ve Anglo-Nubian Teke Sperması Kriyoprezervasyonunda Soya Lesitini ve Lipozom Bazlı Sperma Sulandırıcılarının Karşılaştırmalı Etkinliğinin Belirlenmesi

Öz

Bu çalışma, Boer ve Anglo-Nubian ırkı tekelerde, farklı etki mekanizmalara sahip iki hayvansal kökenli olmayan sulandırıcının çözdürme sonrası spermatolojik özellikler üzerindeki etkilerini belirlemek amacıyla gerçekleştirildi. On baş tekeden (Boer, n=5; Anglo-Nubian, n=5) haftada bir kez olmak üzere toplam altı tekrarlı ejakülat toplandı. Her örnek bireysel olarak kriyoprezervasyona tabi tutuldu. Her ejakülat iki eşit kısma ayrılarak, biri soya lesitini bazlı AndroMed, diğeri ise lipozom bazlı OptiXcell ile sulandırıldı. Dondurma ve çözdürme işlemlerinin ardından total ve progresif motilite (%) ile kinematik parametreler Bilgisayar Destekli Sperm Analizi (CASA) sistemiyle değerlendirildi. Ayrıca, akış sitometrisi kullanılarak plazma membranı bütünlüğü, akrozom bütünlüğü, mitokondriyal membran potansiyeli ve süperoksit radikallerinin miktarı belirlenerek oksidatif stres durumu analiz edildi. Elde edilen sonuçlara göre, Anglo-Nubian örneklerinde her iki sulandırıcı arasında CASA ve akış sitometrisi parametreleri bakımından anlamlı bir fark saptanmadı (p>0.05). Buna karşın, Boer ırkında AndroMed ile sulandırılan grupta total ve progresif motilite değerleri ile VAP, VSL, VCL, ALH ve BCF parametreleri daha yüksek bulundu (p<0.05). Ayrıca, Boer ırkına ait AndroMed ile sulandırılan örneklerde oksidatif stres düzeyleri, OptiXcell grubuna kıyasla daha düşük belirlendi (p<0.05). Bu bulgular, sulandırıcı etkinliğinin ırka bağlı olduğunu göstermektedir. AndroMed, Boer tekelerinde üstün performans sergilerken, her iki sulandırıcı da Anglo-Nubian tekelerinde benzer sonuçlar vermiştir. Irka özgü yanıt farkı, soya lesitini ve lipozom temelli sulandırıcılara karşı farklı ırk tepkilerini karşılaştırmalı olarak ortaya koymakta ve ırka özgü dondurma protokollerinin gerekliliğini desteklemektedir. Sonuç olarak, bu çalışma, uygun sulandırıcı seçiminin çözdürme sonrası sperma kalitesinde kritik bir rol oynadığını ve ırka özgü uyumun kriyoprezervasyon protokollerinin geliştirilmesinde temel bir kriter olduğunu ortaya koymaktadır.

Anahtar Kelimeler

• Anglo-Nubian
• Boer
• Kriyoprezervasyon
• Sperma sulandırıcısı
• Teke spermi

Comparative Effectiveness of Soya Bean Lecithin and Liposome Based Semen Extenders in Cryopreservation of Boer and Anglo-Nubian Goat Semen

Öz

This study investigated the effects of two commercially available non-animal-derived extenders with different mechanisms of action on post-thaw spermatological characteristics in Boer and Anglo-Nubian goat breeds during the non-breeding season. Ejaculates were collected weekly from ten goats (Boer, n=5; Anglo-Nubian, n=5) over six replicates, with individual cryopreservation performed for each sample. Each ejaculate was divided into two aliquots: one diluted with soy lecithin-based AndroMed and the other with liposome-based OptiXcell prior to cryopreservation. Post-thaw assessment included Computer-Assisted Sperm Analysis (CASA) for total and progressive motility (%) and kinematic parameters, while flow cytometry evaluated plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, and quantified superoxide radicals to determine oxidative stress status. Results revealed that Anglo-Nubian samples exhibited comparable CASA and flow cytometry parameters between extenders (p>0.05). However, Boer samples demonstrated significantly higher total and progressive motility, as well as elevated VAP, VSL, VCL, ALH, and BCF values in the AndroMed-diluted group (p<0.05). Additionally, oxidative stress levels were significantly lower in Boer semen extended with AndroMed compared to OptiXcell (p<0.05). These results indicate breed-dependent extender efficacy, with AndroMed exhibiting superior performance in Boer goats while both extenders yielded equivalent outcomes in Anglo-Nubian goats. This breed-specific response pattern provides comparative evidence of differential breed responses to soy lecithin- and liposome-based extenders, directly supporting the need for breed-tailored semen preservation strategies. Consequently, these findings demonstrate that extender selection critically determines post-thaw sperm quality, confirming breed-specific compatibility as an essential criterion for optimal semen cryopreservation protocols.

Anahtar Kelimeler

• Anglo-Nubian
• Boer
• Cryopreservation
• Goat sperm
• Semen extender

Kaynakça

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