Burdur İlinde Solunum Sistemi Enfeksiyonu Klinik Bulguları Gösteren Sığırlarda Influenza D Virusunun Moleküler Tespiti

Öz

Influenza D virus (IDV) çoğunlukla Bovine Respiratory Disease Complex (BRDC) etiyolosinde yer alan önemli bir viral patojendir. Bu araştırmada halk elinde yetiştirilen sığırların solunum sistemi enfeksiyonu etiyolojisinde IDV’nin rolü ve prevalansının belirlenmesi amaçlanmıştır. Bu nedenle araştırma bölgesindeki solunum sistemi enfeksiyonu klinik bulguları gösteren farklı ırk, yaş, cinsiyete sahip ve etkene karşı aşılanmamış 200 sığırdan toplanan nazal swap örneklerinde etkenin Hemagglutinin-Esterase-Fusion (HEF) gen bölgesine özgü oligonükleotid primer çifti kullanılarak konvensiyonel RT-PCR yöntemi ile moleküler tespiti gerçekleştirilmiştir. Sonuç olarak 1,5% (3/200; %95 CI: 0,3-4,3) prevalans oranı tespit edilmiştir. Bununla birlikte etken tespiti yapılan işletmelere ithal hayvan alımı yapıldığı ve sürüye yeni katılan hayvanların uygun bir karantina dönemi geçirmediği tespit edilmiştir. Araştırma sonuçları dünya genelinde gerçekleştirilen prevalans ve deneysel klinik çalışmalarla benzerlik göstermektedir. Türkiye'de sığırlarda IDV dolaşımını araştıran moleküler çalışmaların sınırlı sayıda olması göz önüne alındığında, bu çalışma Burdur ilinden ek epidemiyolojik veriler sunmaktadır.

Anahtar Kelimeler

• Sığır
• İnfluenza D virus
• RT-PCR
• Prevalans

Destekleyen Kurum

Bu araştırma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından 1002/A Hızlı Destek Programı kapsamında (Proje No: 123O192) “Solunum Sistemi Enfeksiyonlu Sığırlarda Bovine Parainfluenza Virus Tip 3, Sığır Respiratory Syncytial Virus ve Influenza D Virusun Moleküler Olarak Araştırılması ve Filogenetik Analizi” başlıklı proje olarak desteklenmiştir.

Proje Numarası

123O192

Etik Beyan

Burdur Mehmet Akif Ersoy Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’ndan onay alınmıştır. Bu çalışmada kullanılan prosedürler, Helsinki Bildirgesi’nde belirtilen ilkelerle uyumludur (MAKU-HADYEK 1098/26.04.2023).

Molecular Detection of Influenza D Virus in Cattle with Clinical Signs of Respiratory Infection in Burdur Province, Türkiye

Öz

Influenza D virus (IDV) is an important viral pathogen commonly associated with the etiology of Bovine Respiratory Disease Complex (BRDC). This study aimed to investigate the role and prevalence of IDV in the etiology of respiratory system infections in cattle bred by farmers in the region. Therefore, molecular detection of the agent was carried out using the conventional RT-PCR method with oligonucleotide primers specific to the Hemagglutinin-Esterase-Fusion (HEF) gene region in nasal swab samples obtained from 200 cattle of different breeds, ages, and sexes with clinical symptoms of respiratory system infection in the research area. As a result, a prevalence rate of 1.5% (3/200; %95 CI: 0.3-4.3) was reported. Furthermore, it was determined that affected herds contained imported animals and that newly introduced animals had not undergone an appropriate quarantine procedure. The research results are consistent with prevalence and experimental clinical studies conducted worldwide. Considering the limited molecular studies investigating IDV circulation in cattle in Türkiye, this study provides additional epidemiological data from the province of Burdur.

Anahtar Kelimeler

• Cattle
• Influenza D Virus
• RT-PCR
• Prevalence

Destekleyen Kurum

This research was supported by Scientific and Technological Research Council of Türkiye (TUBİTAK) with 1002/A Rapid support module (Project No: 123O192) as a "Molecular Investigation and Phylogenetic Analysis of Parainfluenza Virus Type 3, Bovine Respiratory Syncytial Virus and Influenza D Virus in Cattle with Respiratory System Infection”

Proje Numarası

123O192

Etik Beyan

Approval was obtained from the animal testing local ethics committee of Burdur Mehmet Akif Ersoy University. The procedures used in this study adhere to the tenets of the Declaration of Helsinki (MAKU-HADYEK 1098/26.04.2023)

Kaynakça

1. Alvarez, I., Banihashem, F., Persson, A., Hurri, E., Kim, H., Ducatez, M., Geijer, E., Valarcher, J.F., Hägglund, S., & Zohari, S. (2023.) Detection and phylogenetic characterization of ınfluenza D in Swedish cattle. Viruses, 17, 17. https://doi.org/10.3390/v17010017
2. Chiapponi, C., Faccini, S., Fusaro, A., Moreno, A., Prosperi, A., Merenda, M., Baioni, L., Gabbi, V., Rosignoli, C., Alborali, G.L., Cavicchio, L., Monne, I., Torreggiani, C., Luppi, A., & Foni, E. (2019). Detection of a new genetic cluster of Influenza D Virus in Italian cattle. Viruses, 11:,1110. https://doi.org/10.3390/v11121110
3. Ducatez, M.F., Pelletier, C., & Meyer, G. (2015). Influenza D virus in cattle, France, 2011-2014. Emerging Infectious Disease, 21, 368–371. PMID: 25628038
4. Ferguson, L., Olivier, A.K., Genova, S., Epperson, W.B., Smith, D.R., Schneider, L., Barton, K., McCuan, K., Webby, R.J., & Wan, X.F. (2016). Pathogenesis of Influenza D Virus in cattle. Journal of Virology, 90, 5636–5642. https://doi.org/10.1128/JVI.03122-15
5. Flynn, O., Gallagher, C., Mooney, J., Irvine, C., Ducatez, M., Hause, B., McGrath, G., & Ryan, E. (2018). Influenza D Virus in cattle, Ireland. Emerging Infectious Disease, 24, 389–391.https://doi.org/10.3201/eid2402.170759. Fulton, R.W.(2009). Bovine respiratory disease research (1983–2009). Animal Healt Research Review, 10, 131–139. https://doi.org/10.1017/S146625230999017X
6. Gao, H., Sun, W., Lu, P., Li, Y., Ren, J., Xia, Y., Dong, Z., Wang, T., Xia, X., & Gao, Y. (2024). First isolation of influenza D virus from cattle in Northeast China. Microbiology Spectrum, 12, e0037424. PMID: 39046264
7. Gaudino, M., Moreno, A., Snoeck, C.J., Zohari, S., Saegerman, C., O’Donovan, T., Ryan, E., Zanni, I., Foni, E., Sausy, A., Hübschen, J.M., Meyer, G., Chiapponi, C., & Ducatez, M.F. (2021). Emerging Influenza D virus infection in European livestock as determined in serology studies: Are we underestimating its spread over the continent? Transboundry and Emerging Disease, 68, 1125–1135. https://doi.org/10.1111/tbed.13812
8. Hause, B.M., Ducatez, M., Collin, E.A., Ran, Z., Liu, R., Sheng, Z., Armien, A., Kaplan, B., Chakravarty, S., Hoppe, A.D., Webby, R.J., Simonson, R.R., & Li, F. (2013). Isolation of a novel Swine Influenza Virus from Oklahoma in 2011 which is distantly related to Human Influenza C viruses. PLoS Pathogens, 9, e1003176. https://doi.org/10.1371/journal.ppat.1003176

9. Irsik, M., Langemeier, M., Schroeder, T., Spire, M., & Roder, J.D. (2006). Estimating the effects of animal health on the performance of feedlot cattle. The Bovine Practitioner 2006; 65–74.
10. Kirchhoff, J., Uhlenbruck, S., Goris, K., Keil, G.M., & Herrler, G. (2014). Three viruses of the bovine respiratory disease complex apply different strategies to initiate infection. Veterinary Research, 45, 1–12. https://doi.org/10.1186/1297-9716-45-20
11. Küçük, A., & Yildirim, Y. (2022). Antigenic and histopathologic evaluation, with molecular characterization and identification of BPIV3 in cattle with respiratory system infections. Large Animal Review, 28, 123–130. Kwasnik, M., Rola, J., & Rozek, W. (2023). Influenza D in domestic and wild animals. Viruses, 15, 2433. https://doi.org/10.3390/v15122433
12. Leibler, J.H., Abdelgadir, A., Seidel, J., White, R.F., Johnson, W.E., Reynolds, S.J., Gray, G.C., & Schaeffer, J.W. (2023). Influenza D virus exposure among US cattle workers: A call for surveillance. Zoonoses Public Health, 70, 166–170. https://doi.org /10.1111/zph.13008
13. Lim, E.H., Lim, S.I., Kim, M.J., Kwon, M., Kim, M.J., Lee, K.B., Choe, S., An, D.J., Hyun, B.H., Park, J.Y., Bae, Y.C., Jeoung, H.Y., Lee, K.K., & Lee, Y.H. (2023). First detection of Influenza D Virus infection in cattle and pigs in the Republic of Korea. Microorganisms, 11, 1751. https://doi.org/10.3390/microorganisms11071751
14. Lion, A., Secula, A., Rançon, C., Boulesteix, O., Pinard, A., Deslis, A., Hägglund, S., Salem, E., Cassard, H., Näslund, K., Gaudino, M., Moreno, A., Brocchi, E., Delverdier, M., Zohari, S., Baranowski, E., Valarcher, J.F., Ducatez, M.F., & Meyer, G. (2021). Enhanced Pathogenesis Caused by Influenza D Virus and Mycoplasma bovis Coinfection in Calves: a Disease Severity Linked with Overexpression of IFN-γ as a Key Player of the Enhanced Innate Immune Response in Lungs. Microbiology Spectrum, 9, e0169021.https://doi.org/10.1128/spectrum.01690-21
15. Liu, R., Sheng, Z., Huang, C., Wang, D., & Li, F. (2020). Influenza D virus. Current Opinion in Virology, 44, 154–161. https://doi.org/10.1016/j.coviro.2020.08.004
16. MacLachlan, N.J., Dubovi, E.J., Barthold, S.W., Swayne, D.E., & Winton JR (2017). Fenner’s Veterinary Virology 5th ed. Elsevier.
17. Pardon, B., Callens, J., Maris, J., Allais, L., Van Praet, W., Deprez, P., & Ribbens, S. (2020). Pathogen-specific risk factors in acute outbreaks of respiratory disease in calves. Journal of Dairy Science, 103, 2556–2566. https://doi.org/10.3168/jds.2019-17486
18. Qi, J., Huang, F., Gan, L., Zhou, X., Gou, L., Xie, Y., Guo, H., Fang, J., & Zuo, Z. (2024). Multi-omics investigation into long-distance road transportation effects on respiratory health and immunometabolic responses in calves. Microbiome 2024;12(1): 242. Available from: https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-024-01962-2
19. Reed, K.D. (2018). Viral Zoonoses. Reference Modul of Biomedical Sciences. Elsevier. https://doi.org/10.1016/B978-0-12-801238-3.95729-5
20. Rio, D.C., Ares, M., Hannon, G.J., & Nilsen, T.W. (2010). Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harbour Protocol.
21. Rosignoli, C., Faccini, S., Merenda, M., Chiapponi, C., de Mattia, A., Bufalo, G., Garbarino, C., Baioni, L., Bolzoni, L., Nigrelli, A., & Foni E. (2017). Influenza D virus infection in cattle in Italy. Large Animal Review, 23, 123–128. Ruiz, M., Puig, A., Bassols, M., Fraile, L., & Armengol, R. (2022). Influenza D Virus: A review and update of its role in Bovine Respiratory Syndrome. Viruses, 14, 2717. https://doi/10.3390/v14122717
22. Salem, E., Cook, E.A.J., Lbacha, H.A., Oliva, J., Awoume, F., Aplogan, G.L., Hymann, E.C., Muloi, D., Deem, S.L., Alali, S., Zouagui, Z., Fèvre, E.M., Meyer, G., & Ducatez, M.F. (2017). Serologic evidence for Influenza C and D virus among ruminants and camelids, Africa, 1991–2015. Emerging Infectious Diseases, 23, 1556–1559. https://doi/10.3201/eid2309.170342
23. Sederdahl, B.K.,& Williams, J.V. (2020). Epidemiology and clinical characteristics of Influenza C Virus. Viruses, 12, 89. https://doi/10.3390/v12010089
24. Taubenberger, J.K., & Morens, D.M. (2008). The pathology of Influenza Virus infections. Annual Review of Pathology: Mechanisms of Disease, 3, 499–522. https://doi/10.1146/annurev.pathmechdis.3.121806.154316
25. Umar, S., Ahmed, A., Gulraiz, S.H., Muhammad, S., Yu, J., Rasool, A., Koviazina, R., Yilmaz, A., Yilmaz, H., & Anderson, B.D. (2024). First report of Influenza D Virus in dairy cattle in Pakistan. Viruses, 16, 1865. PMID: 39772175
26. Vicosa Bauermann, F., Falkenberg, S., Rudd, J.M., Peter, C.M., Merchioratto, I., Ritchey, J.W., Gilliam, J., Taylor, J., Ma, H., & Maggioli, M.F. (2023). Immune responses to Influenza D Virus in calves previously infected with Bovine Viral Diarrhea Virus 2. Viruses, 15, 2442. https://doi.org/10.3390/v15122442
27. White, S.K., Ma, W., McDaniel, C.J., Gray, G.C., & Lednicky, J.A. (2016). Serologic evidence of exposure to Influenza D virus among persons with occupational contact with cattle. Journal of Clinical Virology, 81, 31–33. https://doi.org/10.1016/j.jcv.2016.05.017
28. Yesilbağ, K., Toker, E.B., Ates O (2022). Recent strains of influenza D virus create a new genetic cluster for European strains. Microbial Pathogenesis, 172, 105769. https://doi.org/10.1016/j.micpath.2022.105769.
29. Yilmaz, A., Umar, S., Turan, N., Aydin, O., Tali, H.E., Oguzoglu, T.C., Yilmaz, H., Richt, J.A., & Ducatez, M.F. (2020). First report of influenza D virus infection in Turkish cattle with respiratory disease. Research in Veterinary Science, 130, 98–102. https://doi.org/10.1016/j.rvsc.2020.02.017
30. Yu, J., Li, F., Wang, D. (2021). The first decade of research advances in influenza D virus. Journal of General Virology, 102, 001529. https://doi.org/10.1099/jgv.0.001529