The Protective Effect of Coenzyme Q10 on Serum Anti-Müllerian Hormone Levels in Rats Treated with Cyclophosphamide

Öz

Anti-Müllerian hormone (AMH) is widely used in veterinary medicine to assess reproductive health. Cyclophosphamide (CP) is a chemotherapeutic and immunosuppressive drug that has been reported to reduce AMH levels and cause damage to ovarian reserves, although findings vary depending on study design. Coenzyme Q10 (CoQ10), known for its antioxidant properties, has been suggested to have a protective role against oxidative stress-related ovarian damage. This study was conducted to examine how CP affects serum AMH levels in rats and whether CoQ10 supplementation could help reduce any negative impact. A total of 35 Wistar Albino rats were used in the study. They were divided into five groups: control, sham, CP, CoQ10, and CP+CoQ10. All substances were administered by oral gavage. CP was dissolved in distilled water and administered at a dose of 6 mg/kg/day, while CoQ10 was dissolved in olive oil and given at a dose of 2.8 mg/kg/day. The treatments lasted for four weeks. Results showed that CP alone did not significantly change AMH levels (p>0.05). In contrast, CoQ10 alone led to a significant increase in serum AMH levels (p<0.05). However, when CP and CoQ10 were given together, no significant difference in AMH levels was observed. This may suggest that CP interferes with or masks the effect of CoQ10 on the ovaries. These findings may contribute to a better understanding of the interaction between CP and CoQ10, especially in terms of dosage, duration and timing of treatment, in future studies.

Anahtar Kelimeler

• AMH
• Cyclophosphamide
• Coenzyme Q10
• Rat

Siklofosfamid ile Tedavi Edilen Ratlarda Serum Anti-Müllerian Hormon Düzeyleri Üzerinde Koenzim Q10'un Koruyucu Etkisi

Öz

Anti-Müllerian hormon (AMH), veteriner hekimlikte reprodüktif sağlığını değerlendirmek için yaygın olarak kullanılmaktadır. Siklofosfamid (CP), AMH düzeylerini düşürdüğü ve ovaryum rezervlerine zarar verdiği bildirilen kemoterapötik ve immünosüpresif bir ilaçtır. Ancak bulgular çalışma tasarımına bağlı olarak değişiklik göstermektedir. Antioksidan özelliğiyle bilinen Koenzim Q10'un (CoQ10), oksidatif strese bağlı ovaryum hasarına karşı koruyucu bir rolü olduğu öne sürülmektedir. Bu çalışma, CP'nin sıçanlarda serum AMH düzeylerini nasıl etkilediğini ve CoQ10 takviyesinin herhangi bir olumsuz etkiyi azaltmaya yardımcı olup olamayacağını incelemek amacıyla yürütülmüştür. Çalışmada toplam 35 Wistar Albino sıçanı kullanılmıştır. Sıçanlar beş gruba ayrılmıştır: kontrol, sham, CP, CoQ10 ve CP+CoQ10. Tüm maddeler oral gavaj yoluyla uygulanmıştır. CP, distile suda çözülerek 6 mg/kg/gün dozunda uygulanırken, CoQ10 zeytinyağında çözülerek 2,8 mg/kg/gün dozunda uygulandı. Tedaviler dört hafta sürdü. Sonuçlar, CP'nin tek başına AMH seviyelerinde anlamlı bir değişiklik oluşturmadığı görüldü (p > 0,05). Buna karşılık, CoQ10 tek başına serum AMH seviyelerinde anlamlı bir artışa yol açtı (p<0,05). Ancak CP ve CoQ10 birlikte uygulandığında, AMH seviyelerinde anlamlı bir fark gözlenmedi. Bu durum, CP'nin CoQ10'un ovaryum üzerindeki etkisini engellediğini veya maskelediğini düşündürdü. Bu bulgular, özellikle dozaj, tedavi süresi ve zamanlaması açısından, CP ve CoQ10 arasındaki etkileşimin gelecekteki çalışmalarda daha iyi anlaşılmasına katkıda bulunabilir.

Anahtar Kelimeler

• AMH
• Koenzim Q10
• Sıçan
• Siklofosfamid

Kaynakça

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