Age-Related Comparison of Serum Symmetrical Dimethylarginine Levels and Urea, Creatinine Levels in Hair Goats

Öz

Symmetric dimethylarginine (SDMA) is an important marker for determining renal function in both humans and animal models. It has been shown that cardiovascular risk factors additional to renal dysfunction may affect SDMA plasma levels. Hair goats are the most bred breed in goat breeding in Turkey. In this study, it was aimed to investigate the age-related changes in blood serum SDMA, Urea and Creatinine levels in Hair goats. 40 female Hair goats, housed under the same care and feeding conditions, were used in the study. The study groups were grouped as 0-12 months (Group 1), 1-2 years (Group 2), 2-3 years (Group 3), and 3-4 years (Group 4), with 10 goats in each group. Symmetric Dimethyl Arginine levels in blood serum samples was measured with the commercial ELISA Kit. Serum urea and creatinine levels were measured with an autoanalyzer. SDMA levels in the blood serum of female goats in groups 1, 2, 3 and 4 were 2,43±0,24, 3.21±0.44, 3.74±0.32 and 4.46±0.59 (p =0.013); Urea levels (mg/dL) were 20±1.87, 24.5±1.43, 24.4±1.82 and 19.1±1.75 (p=0.058); Creatinine levels (mg/dL) were 0.35±0.027, 0.32±0.020, 0.34±0.022, 0.36±0.022 (p=0.648) respectively. While the SDMA levels (ng/mL) obtained in groups 3 and 4 were found to be statistically significantly higher than the values obtained in groups 1 and 2 (p<0.05), no significant difference was found between urea and creatinine values. The findings contribute to the literature knowledge on both the evaluation of SDMA levels and its comparation together with urea and creatinine levels in female goats of different ages.

Anahtar Kelimeler

• Creatinine
• Hair goat
• Symmetric Dimethylarginine
• Urea

Destekleyen Kurum

This work was supported by the Burdur Mehmet Akif Ersoy University Scientific Research Projects Coordination Unit under the graduate thesis project numbered 0933-YL-23.

Proje Numarası

0933-YL-23

Etik Beyan

Permission for the animal material used in the study was obtained from the MAKÜ Local Ethics Committee for Animal Experiments with the decision dated 29.03.2023 and numbered 1038.

Teşekkür

We would like to thank Doğuş Özalkan for his support

Kıl Keçilerinde Serum Simetrik Dimetilarjinin Düzeyleri ile Üre, Kreatinin Düzeylerinin Yaşa Bağlı Karşılaştırılması

Öz

SDMA insan hem de hayvan modellerinde böbrek fonksiyonunu belirlemede önemli bir belirtecidir. Böbrek fonksiyonu bozukluğu dışındaki kardiyovasküler risk faktörlerinin de SDMA plazma düzeylerini etkileyebileceği gösterilmiştir. Farklı iklim koşullarına ve arazi tiplerine iyi adapte olan Kıl keçileri, Türkiye’de keçi yetiştiriciliğinde % 96 oran ile en çok yetiştirilen ırktır. Bu tez çalışmasında kıl keçilerinde kan serum Simetrik dimetilarjinin (SDMA), Üre ve Kreatinin düzeylerinin yaşa bağlı değişimlerinin araştırılması amaçlandı. Çalışmada aynı bakım ve besleme şartları altında barındırılan 40 adet dişi Kıl keçisi kullanıldı. Çalışma grupları, grupta 10 adet keçi olacak şekilde 0-12 ay (Grup 1), 1-2 yaş (Grup 2), 2-3 yaş (Grup 3), ve 3-4 yaş (Grup 4), olarak gruplandırıldı. Kan serum örneklerinde Simetrik Dimetil Arjinin analizi tayini ticari kit (SDMA) ELISA Kit protoküle uygun olarak yapıldı. Serum üre ve kreatinin düzeyleri ise otoanalizörde ölçüldü. Dişi keçilerde kan serumunda grup 1, 2, 3 ve 4 ‘de sırasıyla SDMA düzeyleri (ng/mL) 2,43±0,24, 3,21±0,44, 3,74±0,32 ve 4,46±0,59 (p=0,013); Üre düzeyleri (mg/dL) 20±1,87, 24,5±1,43, 24,4±1,82 ve 19,1±1,75 (p=0,058); Kreatinin düzeyleri (mg/dL) 0,35±0,027, 0,32±0,020, 0,34±0,022, 0,36±0,022 (p=0,648) olarak belirlenmiştir. Grup 3 ve 4’de elde edilen SDMA düzeyleri, grup 1 ve 2 ‘de elde edilen değerler göre değerlere göre istatistik olarak anlamlı yüksek bulunurken (p<0.05), üre ve kreatinin değerleri arasında anlamlı bir fark bulunamamıştır. Elde edilen bulgularla, hem farklı yaşlardaki dişi keçilerde SDMA düzeylerinin üre, kreatinin düzeyleri ile birlikte değerlendirilmesi hem de keçilerde SDMA düzeyleri konularında literatür bilgisine katkı sağlamaktadır.

Anahtar Kelimeler

• Kıl keçisi
• Kreatinin
• Simetrik Dimetilarjinin
• Üre

Destekleyen Kurum

Bu Araştırma Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından 0933-YL-23 proje numarası ile desteklenmiştir.

Proje Numarası

0933-YL-23

Etik Beyan

Çalışma kullanılan hayvan materyali için, MAKÜ Hayvan Deneyleri Yerel Etik Kurul Başkanlığından 29.03.2023 tarih ve 1038 sayılı karar ile izin alınmıştır.

Teşekkür

Desteklerinden dolayı Doğuş Özalkan'a teşekkür ederiz.

Kaynakça

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