Deksametazon Uygulaması Yapılan Ratlarda Alendronat ve Vitamin D Uygulamalarının Biyokimyasal Parametrelere Etkileri

Öz

İmmünosüpresif ve antienflamatuar etkileriyle tedavi amacıyla sıklıkla tercih edilen glukokortikoidlerin kullanıma bağlı olarak kas-iskelet sistemi, endokrin sistem, kardiyovasküler sistem ve merkezi sinir sistemi gibi birçok sistemde önemli yan etkilerinin olabileceği bilinmektedir. Bu çalışma ile ratlarda deksametazon uygulaması ve bunun olası etkilerini azaltmak amacı ile alendronat ve vitamin D’nin tek başına ya da kombine uygulamalarının çeşitli biyokimyasal parametrelere etkileri araştırılmıştır. Toplam 40 adet rat 5 gruba ayrılarak 6 hafta sürdürülen çalışmada kontrol grubu dışındaki tüm gruplara haftada bir kez deri altı 7.5 mg/kg deksametazon uygulandı. Kontrol grubuna herhangi bir uygulama yapılmazken, deksamatazon uygulanan grupta herhangi bir tedavi gerçekleştirilmedi. Kalan son üç gruba tedavi amacıyla sırasıyla alendronat (haftada 5 gün 0. 9 mg/kg), vitamin D (10.000 UI/500 µL) ve aynı dozlarda alendronat /vitamin D kombinasyonu gavaj yolu ile verildi. Deksametazon uygulamasıyla serum LDH aktivitesi, trigliserid, magnezyum ve demir düzeylerinin kontrol grubuna göre önemli düzeyde (p<0.05) arttığı, klor (p<0.05) düzeylerinin ise düştüğü gözlendi. LDH aktivitesi ve demir düzeylerindeki artış (p<0.05) alendronat uygulamasıyla devam etti. Tüm tedavi gruplarında trigliserid düzeyleri anlamlı derecede (p<0.05) daha yüksek bulundu. Tüm gruplarda kontrol grubuna göre gözlenen albümin düzeylerindeki düşüşün ise alendronat ve kombine kullanımda anlamlı olduğu, üre düzeylerindeki artışın ise sadece vitamin D grubunda anlamlı olduğu bulundu. Deksametazon grubunda gözlenen klor düzeylerindeki anlamlı düşüşün alendronat grubunda, magnezyum düzeylerindeki yüksekliğin ise alendronat ve vitamin D gruplarında devam ettiği gözlendi. Sonuç olarak deksametazonun çeşitli biyokimyasal parametrelere olan etkilerinin giderilmesinde farklı doz ve sürelerde alendronat ve vitamin D uygulamaların yapıldığı yeni çalışmalara gereksinim duyulduğu düşünülmüştür.

Anahtar Kelimeler

• Deksametazon
• Alendronat
• Vıtamın D
• Rat
• Biyokimyasal parametreler

The Effects of Alendronate and Vitamin D Administration on Biochemical Parameters in Rats Treated with Dexamethasone

Öz

Glucocorticoids, frequently preferred for treatment due to their immunosuppressive and anti-inflammatory effects, are known to have significant side effects on many systems, such as the musculoskeletal system, endocrine system, cardiovascular system, and central nervous system, depending on their use. This study investigated the effects of dexamethasone administration in rats and the effects of alendronate and vitamin D, administered alone or in combination to reduce its potential effects, on various biochemical parameters. A total of 40 rats were divided into 5 groups and administered 7.5 mg/kg dexamethasone subcutaneously once a week for 6 weeks, in all groups except for the control group. No treatment was administered to the control group, and no treatment was administered to the group receiving dexamethasone. The remaining three groups received treatment with alendronate (0.9 mg/kg 5 days per week), vitamin D (10.000 IU/500 µL), and alendronate/vitamin D combination administered at the same doses via gavage, respectively. With dexamethasone administration, serum LDH activity, triglyceride, magnesium, and iron levels significantly increased (p<0.05) compared to the control group, while chloride levels lowered (p<0.05). The increase in LDH activity and iron levels (p<0.05) also continued with alendronate administration. Triglyceride levels were significantly higher (p<0.05) in all treatment groups. The decrease in albumin levels observed in all groups compared to the control group was significant with alendronate and combined use, while the increase in urea levels was significant only in the vitamin D group. The significant decrease in chloride levels observed in the dexamethasone group was observed to continue in the alendronate group, while the high magnesium levels continued in the alendronate and vitamin D groups. In conclusion, it is thought that further studies are needed in which alendronate and vitamin D are administered at different doses and durations to eliminate the effects of dexamethasone on various biochemical parameters.

Anahtar Kelimeler

• Dexamethasone
• Alendronate
• Vitamin D
• Rat
• Biochemical parameters

Destekleyen Kurum

“This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.”

Etik Beyan

This study ethical approval (Approval no: 2025/119, date 30.12.2025) was obtained from Selcuk University Experimental Medicine And Application Center Ethics Committee (SÜDAM).

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