Farklı Üreme Yaşındaki Keçilerde Dondurulmuş Sperma ile Servikal Tohumlama

Öz

Keçide dondurulmuş sperma ile servikal suni tohumlama (ST) uygulaması düşük gebelik oranları nedeniyle halen sınırlıdır. Bu çalışmanın amacı iki farklı üreme yaşındaki Saanen keçilerinde donmuş sperma kullanımının etkinliğini belirlemekti. Senkronizasyon ve gebelik oranlarının etkileri de incelenmiştir. Doksan sekiz (98) dişi Saanen keçisi iki gruba ayrıldı: Grup-1 = 49 (7 aylık) ve Grup-2 = 49 (19 aylık) keçi. Östrus 11 gün süresince intravajinal sünger (30 mg;  fluogestone acetate, FGA) ve süngerin çıkarılmasından 48 saat önce 50 ug kloprostenol (PGF2a) ve gebe kısrak serum gonadotropini (PMSG, 500 IU) ile senkronize edildi. Sabit zamanlı servikal tohumlama dondurulmuş sperma ile üreme mevsiminde süngerlerinin çıkarılmasından 43-45 saat sonra gerçekleştirildi. Östrus arama tekesi ile tespit edildi. Grup 1 ve Grup 2'de östrus görülme oranları sırasıyla %71.4, %63.3 olarak tespit edildi (p>0.05). Altmışaltı (66) keçi dondurulmuş-çözdürülmüş spermayla tohumlandı. Gebelik abdominal ultrasonografi ile belirlendi. Gebelik oranları (Grup-1: %31.4, Grup-2: %32.3) yaş grupları arasında anlamlı bir farklılık göstermedi (p>0.05). Ayrıca, artan çoklu doğum ve azalan embriyonik kayıplar sayesinde Grup-1’de (%145.5), Grup-2’ye (%90.0) göre daha yüksek oranda yavru elde edilmiştir. Sonuç olarak, Saanen keçileri, 7 aylık yaştan itibaren suni tohumlama programlarına alınabilir ve yüksek senkronizasyon başarısı ve yavru verimine gibi birçok fayda sağlayabilir.

Anahtar Kelimeler

• Dondurulmuş sperma,fertilite,gebelik,üreme yaşı,suni tohumlama

Cervical Insemination with Frozen Thawed Semen in Goats at Different Breeding Age

Öz

Artificial insemination (AI) in goat is currently limited by the poor fertility obtained following the cervical insemination with frozen-thawed semen. The objective of the present study was to determine the efficiency of frozen semen use in two different breeding ages at Saanen goats. The effects of synchronization and fertility rates were also examined. Ninety-eight (98) Saanen goats were divided into two groups: Group-1 = 49 (7-months-old) and Group-2 = 49 (19-month- old) does. Estrus was synchronized by impregnated intravaginal sponges (30 mg; fluogestone acetate, FGA), and i.m. administration of 50 µg of cloprostenol (PGF2α) and pregnant mare serum gonadotropin (PMSG, 500 IU), at 48 h before sponge removal. Cervical fixed time AI was carried out with frozen semen 43 h after removal of the sponges during the breeding season. Estrus was detected by teaser buck. The occurrence of estrus in Group-1 and Group-2 were 71.4%, 63.3%, respectively (p>0.05). Sixty-six (66) goats were inseminated with frozen‐thawed semen. The pregnancy rate was determined by abdominal scanning. Pregnancy rates were (Group-1: 31.4%, Group-2: 32.3%) not differed significantly between age groups (p>0.05). Besides, higher lambing rates were obtained in Group-1 (145.5%) than in Group-2 (90.0%) due to increased prolificacy and reduced embryonic failure. In conclusion, Saanen goats in younger ages, starting from the 7 months old age could be included in the breeding programs and could provide several benefits such as higher percentages of synchronization success and prolificacy. 

Anahtar Kelimeler

• Artificial Insemination,breeding age,frozen semen,pregnanc,fertility

Kaynakça

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