Streptozotosin ile Diyabet Oluşturulan Ratlarda Beyaz Dut Ekstraktının Performans, Antienflamatuar ve Serum Antioksidan Parametreleri Üzerine Etkisi

Yıl 2020, , 1 - 10, 31.03.2020

Zekeriya Safa İnanç , Harun Karaca , Volkan İpek , Burcu Menekşe Balkan Orhan Çorum , Şükrü Güngör , Muhammed İnanç , Halit İmik , Ayhan Ata

https://doi.org/10.30607/kvj.643431

Öz

Bu çalışmada streptozotosin uygulanarak diyabet oluşturulan ratlarda
beyaz dut ekstraktının kan serumlarında antioksidan etkileri, karaciğer dokusu
histopatoloji, antienflamatuar özelliği ve ratlarda performans parametreleri
üzerine etkisi araştırıldı. Deney grupları, kontrol grubu (K, n:5),
streptozotosin uygulanan deney grubu 1 (DG 1, n:6), dut ekstraktı uygulanan
deney grubu 2 (DG 2, n:7), streptozotosin
ve dut ekstraktının birlikte uygulandığı deney grubu 3 (DG 3, n:6) olmak üzere
toplam 24 adet hayvandan oluşturuldu. Karaciğer dokusunun yapısındaki vasküler
konjesyon K ve DG 2’de 2 hayvanda, DG 3’te 1 hayvanda tespit edilmesine rağmen,
DG 1’de bütün hayvanlarda belirlenmiştir (P<0,05). Karaciğer dokusunun yangı
bulguları K’da görülmezken, DG 2’de 2 adet hayvanda hafif şekilde, DG 1’deki
tüm hayvanlarda gözlenmiştir. Karaciğer dokusundaki dejenerasyon kontrol
grubunda görülmezken, DG 2’de 1 adet, DG 1’de ise tüm hayvanlarda görülmüştür
(P<0,01). Grupların serum dokularındaki seruloplazmin düzeyleri arasında
önemli bir fark bulunmazken (P>0,05), Paraoksonaz (PON) aktiviteleri
arasındaki fark önemli düzeyde olduğu tespit edilmiştir (P<0,05). Canlı ağırlık artışı kontrol grubunda 150,03 g., DG 2 de
114,93g. olarak gerçekleşmiştir (P<0,01). DG
1’de deneme süresince 1.33 g. canlı ağırlık artışı olurken DG 3’te ağırlık
kaybı olmuştur (P>0,05). Sonuç olarak araştırmada
ratlara uygulanan dut ekstraktının
karaciğer dokusunda vasküler konjesyon, yangı ve dejenerasyon olgularını önemli
derecelerde azalttığı ve antienflamatuar etkisi gösterebileceği
söylenebilir.

Anahtar Kelimeler

Antienflamatuar, Antioksidan, Beyaz dut ekstraktı, performans, yangı

Effect of White Mulberry Extract on Performance, Anti-inflammatory and Serum Antioxidant Parameters in Diabetes-induced Rats with Streptozotocin

Öz

This study was conducted to investigate the effect of the white mulberry extract on histopathology, anti-inflammatory properties of liver tissue, blood serum antioxidant parameters and performance parameters of diabetes-induced rats with streptozotocin. Experimental groups consist of 24 animals which were divided into four groups; control group (C, n: 5), streptozotocin applied group 1 (DG 1, n: 6), mulberry extract applied group 2 (DG 2, n: 7), streptozotocin and mulberry extract experimental group 3 (DG 3, n:6), totally 24 animals were used. Although vascular congestion in the liver tissue was detected 2 animals in C and DG 2 groups and 1 animal in DG 3 group, vascular congestion was detected in all animals in DG 1 group. (P <0.05). Inflammatory findings of liver tissue were not seen in C, whereas it was mild in 2 animals in DG 2 and it was seen in all animals in the group of DG 1. Degeneration findings in liver tissue were not seen in C, but it was detected in 1 animal of DG 2 and all animals in DG 1 (P <0.01). There was no statistical difference between the ceruloplasmin levels of the groups (P> 0.05).  Live weight gain in control group 150,03 g., DG 2 114.43 g. (P <0,01). 1.33 g. live weight gain and weight loss in DG 3 (P> 0.05). In conclusion, mulberry extract applied to rats significantly reduced vascular congestion, inflammation and degeneration findings in liver tissue and it can be said that mulberry extract has antiinflammatory effect.

Anahtar Kelimeler

Anti-inflammatory, Antioxidant, İnflammation, Performance, White Mulberry Extract

Kaynakça

• Alam AHMK, Rahman MAA, Baki MA, Rashid MH, Bhuyan MSA, Sadik G. Antidiarrhoeal principle of Achyranthes ferruginea Roxb. and their cytotoxicity. Pharm. J. 2002; 12,1–4.
• Alı AB, Zhang Q, Lım YK, Fang D, Retnam L. Expression of major HDL-associated antioxidant. PON-1 is gender and regulated during inflammation. Free Rad Bio & Me. 2003; 34: 824-829
• Ameen AM, Hapipah MA, Abdul AK, Mohd NS, Salmah I. Evaluation of the antiulcer activities of Morus alba extracts in experimentally induced gastric ulcer in rats.Biomed Res. 2009; 20, 35–39.
• Anonymous. The wealth of India, a dictionary of raw materials and industrial products, Raw materials. CSIR. 2001; 2950, 429–437.
• Arabshahi-Delouee S, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 2007; 102, 1233–1240.
• Armstong D. Advanced Protocols in Oxidative Stres-Humana Press, New York. 2008.
• Balakrishnan CR, Goswami SL. Biochemical polymorphism in river buffalo. In Buffalo and goats in Asia, genetic diversity and its application. NM Tulloh (Ed), Proceeding of a seminar Kuala Lumpur, Malaysia. 1991; No, 34, 20-27.
• Boles A, Kandimalla R, Reddy PH. Dynamics of diabetes and obesity: Epidemiological perspective. Biochim. Biophys. Acta - Mol. Basis Dis. Elsevier B.V.; 2017. p. 1026–36.
• Ceron JJ, Martínez-Subiela S. An automated spectrophotometric method for measuring canine ceruloplasmin in serum. Vet Res. 2004; 35 (6): 671-679.
• Choi EM, Hwang JK. Effects of Morus alba leaf extract on the production of nitric oxide prostaglandin E2 and cytokines in RAW2647 macrophages. Fitoterapia. 2005; 76, 608–613.
• Curzon G, Vallet L. The prufication of human ceruloplasmin. Biochem J. 1960; 74, 279-287.
• Demirel M. İnsülin Tedavisi Başlanan Diabet Hastalarında Kilo Değişimi ve Bumu Etkileyen Parametrelerin İrdelenmesi. T.C Sağlık Bakanlığı Şişli Eftal Eğitim ve Araştırma Hastanesi Aile Hekimliği Şefliği. Uz Tez. 2009; 5.
• Dik B, Bas AL, Yazıhan N. The effect ofmidkine on growth factors and oxidative status in an experimental wound modelin diabetic and healthy rats. Canadian Journal of Physiology and Pharmacology. 2017; 95(5): 604-609.
• Du J, He ZD, Jiang R.W, Ye WC, Xu HX, But PPH. Antiviral flavonoids from the root bark of Morus alba L. Phytochemistry. 2003; 62, 1235–1238.
• Enkhmaa B, Shiwaku K, Katsube T, Kitajima K, Anuurad E, Yamasaki M. Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient mice. J Nutr. 2005; 135, 729–734.
• Fleming RE, Whi tınan IP, Gitliıı JD. Introduction of ceruloplasmin gene expession in rat lung during inflammation and hyperoxida.American Journal of Physiology. 1991; 260, 68-74.
• Fox PL, Mukhopadhyay C, Ehreııwald E. Structure, oxidant activity and cardiovascular mechanisms ofhuman ceruloplasmin. Life Science. 1995; 56, 21, 1749-58.
• Halliwell B. Reactive oxygen species in living systems, source, biochemistry and role in human disease. American Journal of Medicine. 1991; 30, 91, 3, 14-22.
• Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem. 2006; 97, 25–31.
• Kikuchi T, Nihei M, Nagai H, Fukushi H, Tabata K, Suzuki T, Akilhisa T. Albanol A from the root bark of Morus alba L. induces apoptotic cell death in HL60 human leukemia cell line. Chem Pharm Bull. 2010; 58, 568–571.
• Kim SH, Kim NJ, Choi JS, Park JC. Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata bureau. J Korean Soc Food Sci Nutr. 1993; 22, 68–72.
• Kuo CC, Liu TW, Chen LT, Shiah HS, Wu CM, Cheng YT, Pan WY, Liu JF, Chen KL, Yang YN, Chen SN, Chang JY. Combination of arsenic trioxide and BCNU synergistically triggers redox-mediated autophagic cell death in human solid tumors. Free Radic Biol Med. 2011; 51, 2195–2209.
• Lee YJ, Choi DH, Kim EJ, Kim HY, Kwon TO, Kang DG, Lee HS. Hypotensive, hypolipidemic, and vascular protective effects of Morus alba L. in rats fed an atherogenic diet. Am J Chin Med. 2011; 39, 39–52.
• Mcpearson RA . Clinical Diagnosis and Management by Laboratory Method, In, Henry JB, editör, Philadelphia, W.B. Saunders Company.1996; s. 237-57
• Niidome T, Takahashi K, Goto Y, Goh SM, Tanaka N, Kamei K. Mulberry leaf extract prevents amyloid beta-peptide fibril formation and neurotoxicity. Neuroreport. 2007; 18, 813–816.
• Niumsakul S, Bauer JA. Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity.Basic Clin Pharmacol Toxicol. 2005; 96, 80-87.
• Omar SIA, Amer H, Maher A. Effects of Morus alba leaves extracts on sperm count and testicular weight in experimentally streptozotocin induced diabetes male rats. 3rd Scientific Conference- College of Veterinary Medicine- University of Tikrit. 2016.
• Singab BIR, El-Beshbishy HA, Yonekawa M, Nomura T, Fukai T. Hypoglycemic effect of Egyptian Morus alba root bark extract: effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats.J Ethnopharmacol. 2005; 100, 333–338.
• SPSS. Statistical Packages for the Social Sciences 20th ed. IBM: Chicago. 2011.
• Taylor CR, Cote RJ. Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist, 2nd ed. Philadelphia, WB Saunders. 1994.
• Wang CP, Wang Y, Wang X, Zhang X, Ye JF, Hu LS, Kong LD. Mulberroside a possesses potent uricosuric and nephroprotective effects in hyperuricemic mice.Planta Med. 2011; 77, 786–94.
• Wattanapitayakul SK, Chularojmontri L, Herunsalee A, Charuchong-kolwongse S, Niumsakul S, Bauer JA. Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity. Basic Clin Pharmacol Toxicol. 2005; 96, 80-87.