Molecular Characterization of Echinococcus granulosus Isolates Found in Cattle, Buffaloes, Sheep and Goats in Afyonkarahisar, Turkey

Öz

This study has been carried out to determine the genotypes of Echinococcus granulosus cysts in cattle, buffaloes, sheep and goats raised in Afyonkarahisar region. Cysts were collected from the internal organs of 258 animals, including 65 goats, 71 sheep, 119 cattle and 3 buffaloes infected with hydatid cysts. DNA was isolated from a total of 78 cysts from germinal membranes and protoscoleces extracted from cysts to identify the genotypes of E. granulosus in infected animals. PCR-RFLP was carried out using the Hin6I and StuI restriction enzymes in the ND1 gene and no polymorphism could be determined in all isolates. In the COX1 gene analysis, G1 strain known as domestic sheep strains and 18 different haplotypes were found in all isolates from cattle, buffaloes, sheep and goats. As a result, it was concluded that all isolates of the cattle, buffaloes, sheep and goats grown in Afyonkarahisar region determined in the analyses carried on the COX1 gene were G1 strain.

Anahtar Kelimeler

• Ruminants
• Echinococcus granulosus
• ND1
• COX1
• PCR-RFLP
• DNA Sequencing

Afyonkarahisar’da Sığır, Manda, Koyun ve Keçilerde Bulunan Echinococcus granulosus İzolatlarının Moleküler Karakterizasyonu

Öz

Bu çalışma, Afyonkarahisar yöresinde yetiştirilen sığır, manda, koyun ve keçilerde bulunan Echinococcus granulosus kistlerinin genotiplerinin belirlenmesi amacıyla yapılmıştır. Araştırmada, hidatik kistle enfekte 65 keçi, 71 koyun, 119 sığır ve 3 manda olmak üzere toplam 258 hayvanın iç organlarından kistler toplanmıştır. Enfekte hayvanlardaki E. granulosus genotiplerini belirlemek amacıyla kistlerden çıkarılan germinal membran ve protoskolekslerden toplam 78 kistten DNA izole edilmiştir. ND1 gen bölgesi Hin6I ve StuI restriksiyon enzimleri kullanılarak PCR-RFLP yapılmış ve tüm izolatlarda polimorfizm belirlenememiştir. COX1 gen bölgesi analizinde sığır, manda, koyun ve keçilerden elde edilen izolatların tümünde evcil koyun suşu olarak bilinen G1 suşu ve 18 farklı haplotip bulunmuştur. Sonuç olarak, Afyonkarahisar yöresinde yetiştirilen sığır, manda, koyun ve keçilerde bulunan izolatların COX1 geninde yapılan genetik analizler sonucunda tüm izolatların G1 suşu olduğu kanısına varılmıştır.

Anahtar Kelimeler

• Ruminantlar
• Echinococcus granulosus
• ND1
• COX1
• PCR-RFLP
• DNA Dizileme

Kaynakça

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