Ratlarda Bortezomib Kaynaklı Dalak Hasarı Üzerine Berberinin Etkisinin Arginaz Aktivitesi ve Oksidan-Antioksidan Parametreler ile İncelenmesi

Öz

Bu çalışmanın amacı; kanser tedavisinde yaygın olarak kullanılan bortezomib (BTZ) kaynaklı dalak hasarına karşı doğal bir flavonoid olan berberinin etkileri araştırmaktır. Bu amaçla çalışmada Sprague Dawley cinsi 35 adet erkek rat kontrol, BTZ, berberin 100 mg/kg, BTZ+berberin 50 mg/kg ve BTZ+berberin 100 mg/kg olmak üzere rastlgele 5 gruba ayrıldı. Analiz sonuçları BTZ’nin glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) gibi antioksidan enzim aktivitelerini ve glutatyon (GSH) düzeylerini azaltarak oksidatif strese neden olduğunu ve böylece lipid peroksidasyonunun son ürünü olan malondialdehit (MDA) seviyelerini artırdığını gösterdi. Bununla birlikte berberinin enzimatik ve non-enzimatik antioksidan düzeylerini artırması yoluyla BTZ kaynaklı oksidatif stresi hafiflettiği ve MDA seviyelerini önemli derecede azalttığı belirlendi. BTZ’nin ayrıca toplam antioksidan kapasite (TAK)’yi azalttığı, toplam oksidan kapasite (TOK) ve nitrik oksit (NO) seviyelerini ise arttığı görülürken berberinin bu parametreleri kontrol grubu seviyelerine yaklaştırdığı tespit edildi. Çalışmada ayrıca arginaz aktivitesi ölçüldü. Sonuçlar incelendiğinde BTZ’nin arginaz aktivitesini baskıladığı, BTZ ile birlikte berberin uygulamasının ise dalak dokusunda arginaz aktivitesini geri kazandırdığı görüldü. Birlikte ele alındığında berberinin dalak dokusunda BTZ’nin tetiklediği oksidatif ve nitrosatif stresi hafifleterek önemli derecede koruma sağladığı ve bu sonuçların daha ileri çalışmalar ile desteklendikten sonra berberinin alternatif tedavi seçenekleri arasına girebileceği düşünülmektedir.

Anahtar Kelimeler

• Berberin
• Bortezomib
• Dalak
• Oksidatif Stres
• Rat

Investigation of the Effect of Berberine with Arginase Activity and Oxidant-Antioxidant Parameters on Bortezomib-Induced Spleen Injury in Rats

Öz

The aim of the study was to investigate the effects of berberine, one of the natural flavonoids, against spleen damage caused by bortezomib (BTZ), which was widely used in the treatment of cancer. Male Sprague-Dawley rats were randomly divided into five groups: a control group, BTZ-treated group, berberine alone treated group 100 mg / kg, , BTZ + berberine 50 mg/kg treated group, and BTZ+berberine 100 mg/kg treated group. Analysis results showed that BTZ caused oxidative stress by reducing antioxidant enzyme activities such as glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) and glutathione (GSH) levels, thereby increasing malondialdehyde (MDA) levels which is the end product of lipid peroxidation. However, by increasing the enzymatic and non-enzymatic antioxidant levels, berberine was found to reduce BTZ-induced oxidative stress and MDA levels significantly. It was also observed that BTZ decreased the total antioxidant capacity (TAC) and increased the total oxidant capacity (TOC) and nitric oxide (NO) levels, while the berberine made these parameters closer to the control group levels. Arginase activity was also measured in the study. When the results were examined, it was seen that BTZ suppressed arginase activity and BTZ and berberine combined administration regained arginase activity. Accordingly, it is thought that the berberine provides significant protection by lessening the oxidative and nitrosative stress triggered by BTZ in the spleen tissue, and after these results are supported by further studies, the berberine can be included among alternative treatment options.

Anahtar Kelimeler

• Berberine
• Bortezomib
• Oxidative Stress
• Rat
• Spleen

Kaynakça

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