Investigation of the Relationship Between Q-Fever Seropositivity and Trace Element Levels in Dairy Cattle

Öz

Query fever (Q fever) which is caused by Coxiella burnetii is a continuing problem as a zoonotic disease in the world. In ruminants, infections are mostly asymptomatic however, abortions and stillbirths may occur during late pregnancy. Trace elements are important for the reproductive performance of ruminants and all have roles in immune function. However, serum trace element levels of Coxiella seropositive infertile and healthy dairy cows have not been investigated yet. The present was aimed to evaluate the trace element status of cattle associated with Coxiella burnetii. For this purpose, 200 dairy cattle with and without clinical problems (infertility, metritis and abortion) were compared. Q fever infection was confirmed with ELISA. Levels of trace elements of samples were analysed utilizing inductively coupled plasma-optical emission spectrophotometer. Serum levels of trace elements of the samples were expressed as μg/mL. In total, 20 of 200 sera were diagnosed to be positive by ELISA. Nine of 20 sera were positive from asymptomatic cattle. Seven of 11 sera were positive from cattle with infertility, while 4 of 11 sera were positive from cattle with abortion. No significant differences were found between trace element levels of ELISA seropositive cattle and asymptomatic seropositive cattle. In conclusion, although statistical analysis of serum trace elements is no significant, our findings clearly show that analysis of trace element levels in cattle may be useful predictors in early treatment and prognosis. Further studies are required to clarify the connection between Coxiella seropositivity, trace elements and clinical symptoms in cattle.

Anahtar Kelimeler

• Cattle
• Trace Elements
• Q Fever
• ELISA

Sütçü Sığırlarda Q hummasının Seropozitifliği ve Eser Elementler Arasındaki İlişkinin Araştırılması

Öz

Q humması, Coxiella burnetii tarafından oluşturulan ve tüm dünyada devam eden zoonoz bir hastalıktır. Ruminantlarda enfeksiyon genellikle asemptomatik seyretmekte, ancak gebeliğin geç döneminde abort ve ölü doğumlar görülebilmektedir. Eser elementler ruminantlarda üreme performansı ve immün fonksiyon üzerinde önemli rol oynar. Coxiella pozitif olan kısır ve sağlıklı süt ineklerinde serum eser element düzeyleri henüz araştırılmamıştır. Bu çalışmanın amacı; sığırlarda Coxiella burnetii ile ilişkili serum eser element düzeylerinin değerlendirilmesidir. Bu amaçla klinik olarak hasta olan (infertilite, metritis, abort) ve olmayan süt sığırları karşılaştırıldı. Q ateşi ELISA yöntemiyle teşhis edildi. Eser element seviyelerinin ölçümü, indüktif olarak eşleşmiş plazma-optik emisyon spektrofotometresi kullanılarak gerçekleştirildi. Serum örneklerinden elde edilen sonuçlar μg/mL cinsinden belirtildi. Toplamda 200 örnekten 20 serum ELISA ile pozitif olarak saptandı. Asemptomatik sığırlardan elde edilen 20 serumun 9’u pozitif olarak tespit edildi. Abort yapan sığırlardan elde edilen 11 serumdan 4’ü pozitifken, infertilitesi olan sığırların7’si pozitif olarak belirlendi. ELISA seropozitif ve asemptomatik seropozitif sığırların serum eser element düzeyleri arasında önemli bir farklılık görülmemiştir. Sonuç olarak; serum eser element seviyelerinde istatistiksel olarak önemli bir fark bulunmamasına rağmen çalışmamızda sığırlarda erken tedavi ve prognozun belirlenmesinde eser element analizlerinin kullanışlı bir yöntem olduğu görüldü. Sığırlarda Coxiella seropozitifliği, eser elementler ve klinik semptomlar arasındaki ilişkinin değerlendirilmesi amacıyla daha fazla çalışma yapılması gerektiği düşüncesindeyiz.

Anahtar Kelimeler

• Sığır
• ELISA
• Eser Elementler
• Q Humması

Kaynakça

1. Andrie S. Is there a role for organic trace element supplements in transition cow health? Vet. J, 2008; 176: 77-83.
2. Angelakis E, Raoult D. Q fever. Vet. Microbiol. 2010; 140(3): 297-309.
3. Arricau-Bouvery N, Rodolais A. Is Q fever an emerging or re-emerging zoonosis? Vet. Res. 2005; 36(3): 32749.
4. Arthington JD. Trace mineral nutrition and the immune response in cattle. Proc “64th Annual Minnesota Nutrition Conference”, Minneapolis, MN. 2005; 106.
5. Ates Alkan F, Karıs D, Cakmak G, Ercan AM. (2019): Analysis of the relationship between hemorheologic parameters, aluminum, manganese, and selenium in smokers. Biol. Trace Elem. Res 2019; 187(1): 22-31.
6. CFSPH (2017) Q fever. Available at http://www.cfsph.iastate.edu/Factsheets/pdfs/q_fever.pdf.: (Accessed Feb 4, 2020).
7. Clemente L, Barahona MJ, Andrade MF, Botelho A. Diagnosis by PCR of Coxiella burnetii in aborted fetuses of domestic ruminants in Portugal. Vet. Record 2009; 164(12): 373-74.
8. Gottsch ML, Murdoch WJ, Van kırk EA. Tumour necrosis factor alpha upregulates matrix mineral metalloproteinase-2 activity in preovulatory ovine follicles metamorphic and endocrine implications. Reprod. Fertil. Develop. 2000; 12: 75-80.

9. Grace ND, Knowles SO. Trace element supplementation of livestock in New Zealand: Meeting the challenges of free-range grazing systems. Vet. Med. Int. 2012; 12: 1-8.
10. Gressley TF. Zinc, copper, manganese, and selenium in dairy cattle rations. Proc “7th Annual Mid-Atlantic Nutrition Conference”2009; pp. 56-71.
11. Hesari BA, Mohri M, Seifi HA. Effect of copper edetate injection in dry pregnant cows on hematology, blood metabolites, weight gain and health of calves. Trop. Ani. Health Prod. 2012; 44(5): 1041-1047.
12. Jones RM, Hertwig S, Pitman J, Vipond R, Aspan A, Bölske G, McCaughey C, McKenna JP, Rotterdam BJ, Bruin A, Ruuls R, Buijs R, Jan Roest H, Sawyer J. Inter laboratory comparison of real-time polymerase chain reaction methods to detect Coxiella burnetii, the causative agent of Q fever. J. Vet. Diagn. Invest. 2011; 23(1): 108-111.
13. McKenna JP, Rotterdam BJ, Bruin A, Ruuls R, Buijs R, Jan Roest H, Sawyer J. Inter laboratory comparison of real-time polymerase chain reaction methods to detect Coxiella burnetii, the causative agent of Q fever. J. Vet. Diagn. Invest. 2011; 23(1): 108-111.
14. Karis D, Tarhan D, Boyacıoglu K, Koksal C, Ercan AM. The comparison of zinc, copper and iron levels in serum, aorta and leftinternal mammarian artery tissues in coronary by-pass graft surgery patients. J. Trace Elem. Med. Biol. 2019; 51: 86-90.
15. Kumar S, Pandey AK, Ahmed W, Razzaque WAA, Dwived DK. Importance of micro minerals in reproductive performance of livestock. Vet. World 2011; 4(5): 230-233.
16. Lang GH. Coxiellosis (Q fever) in animals. In: Q fever. I. The disease Marrie TJ, Ed, Boca Raton, Fla, CRC Press, Inc. 1. 1990; pp. 23-48.
17. Madariaga MG, Rezai K, Trenholme GM, Weinstein RA Q fever: a biological weapon in your backyard. Lancet Infec. Dis. 2003; 3(11): 709-721.
18. Manspeaker JE, Robl MG, Edwards GH, Douglas LW. Chelated minerals: Their role in bovine fertility. Vet. Med. 1987; 82: 951-956.
19. Marrie TJ. Epidemiology of Q fever. In: Marrie TJ (Ed), Q fever, vol 1. The disease. CRC Press, Boca Raton (FL). 1990. pp. 49-70.
20. Marrie TJ. Coxiella burnetii (Q fever) pneumonia. Clin. Infect. Dis. 1995; 21: 253-264.
21. Maurin M, Raoult D. Q Fever. Clin. Microbiol. Rev. 1999; 12: 518-553.
22. Milazzo A, Hall R, Storm PA, Harris RJ, Winslow W, Marmion BP. Sexually transmitted Q fever. Clin. Infect. Dis. 2001; 33(3): 399-402.
23. Sales JNS, Pereira RVV, Bicalho RC, Baruselli PS. Effect of injectable copper, selenium, zinc and manganese on the pregnancy rate of crossbred heifers (Bos indicus × Bos taurus) synchronized for timed embryo transfer. Livest. Sci. 2011; 142: 59-62.
24. Siciliano-Jones JL, Socha MT, Tomlinson DJ, DeFrain JM. Effect of trace mineral source on lactation performance, claw integrity, and fertility of dairy cattle. J. Dairy Sci. 2008; 9: 1985-1995.
25. Spears JW, Weiss WP. Role of antioxidants and trace elements in health and immunity of transition dairy cows. Vet. J. 2008; 176: 70-76.
26. Yildiz H, Akar Y. Relationships between serum progesterone and some mineral levels during the oestrous cycles in cows. Firat Univ. Vet. J. Health1 Sci. 2001; 15: 77-84.