Sisplatin ile Karaciğer Toksisitesi Geliştirilen Ratlarda Zingeron’un Bazı Biyokimyasal Parametreler Üzerine Etkisinin Araştırılması

Öz

Bu çalışmada, ratlarda sisplatin (SP) kaynaklı hepatotoksisite üzerine zingeron’ un (ZO) etkilerinin araştırılması amaçlandı. Çalışmada 35 adet Sprague Dawley erkek rat kullanıldı. Hayvanlar, her grupta 7 rat olacak şekilde 5 gruba ayrıldı. 1.Grup (Kontrol): Sadece oral serum fizyolojik (SF, 7 gün) verildi. 2. Grup (SP): Tek doz 7 mg/kg SP periton içi (i.p.) uygulandı. 3.Grup (ZO): ZO 7 gün boyunca 50 mg/kg/gün dozunda oral verildi. 4. Grup (SP+ZO 25): Tek doz SP uygulamasından 30 dk sonra 25 mg/kg/gün dozunda ZO verilmeye başlandı ve 7 gün devam etti. 5.Grup (SP+ZO 50): Tek doz SP uygulamasından 30 dk sonra 50 mg/kg/gün dozunda ZO verildi ve 7 gün devam etti. Alanin transaminaz (ALT), aspartat transaminaz (AST) ve alkalen fosfataz (ALP) aktivitelerinin SP uygulaması ile arttığı, antioksidan enzimlerden süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (KAT) aktiviteleri ile non-enzimatik antioksidan olan redükte glutatyon (GSH) seviyelerinin SP grubunda düştüğü, malondialdehit (MDA) düzeyinin arttığı görülmüş, ZO’ un ise antioksidan sistemi desteklediği ve MDA düzeylerini azalttığı gözlenmiştir. SP uygulamasının arginaz aktivitesini düşürürken nitrik oksit (NO), 8-hidroksi-2'-deoksiguanozin (8-OHdG), sistein aspartat spesifik proteaz-3 (kaspaz-3), tümör nekroz faktör-alfa (TNF-α), nükleer faktör kappa B (NF-ĸB) ve B-hücreli lenfoma-3 (Bcl-3) seviyelerini artırdığı, ZO’ un bu parametrelerde iyileşme sağladığı saptandı.

Anahtar Kelimeler

• Hepatotoksisite
• İnflamasyon
• Oksidatif stres
• Sisplatin
• Zingeron

Investigation of the Effect of Zingerone on Some Biochemical Parameters on Cisplatin-Induced Liver Toxicity in Rats

Abstract

This study was aimed to investigate the protective effects of zingerone (ZO) on cisplatin (CP)-induced hepatotoxicity in rats. Thirty-five (35) Sprague Dawley male rats were used in the study; the animals were divided into 5 groups of 7 rats in each group. Group 1 (Control): received only oral serum physiologic (SP, for 7 days). Group 2 (CP): received a single dose of 7 mg/kg intraperitoneal (i.p.) of CP. Group 3 (ZO): Received daily oral doses of ZO at 50 mg/kg/day for 7 days. Group 4 (CP+ZO 25): Oral administration of ZO at 25 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Group 5 (CP+ZO 50): Oral administration of ZO at 50 mg/kg/day was started 30 minutes after a single dose CP application and continued for 7 days. Alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) activities increased due to CP administration. Also, enzymatic antioxidants; superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) level were shown to had decreased upon CP administration. After ZO was supported by antioxidant system and decreased malondialdehyde (MDA) levels. It was determined that CP administration increased nitric oxide (NO), 8-hydroxy-2'- deoxyguanosine (8-OHdG), cysteine aspartate specific protease-3 (caspase-3), tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB) and B-cell lymphoma-3 (Bcl-3) levels while arginase activity reduced, ZO administrations could have been the main cause of the improved effects on the parameters.

Keywords

• Cisplatin
• Hepatotoxicity
• Inflammation
• Oxidative stress
• Zingerone.

Kaynakça

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