Determination of Serum Adenosine Deaminase Activity in Bitch Diagnosed as Cystic Endometrial Hyperplasia (CEH)-pyometra Complex

Yıl 2013, Cilt: 6 Sayı: 2, 23 - 28, 01.06.2013

Nilgün Gültiken Gül Fatma Yarım Murat Yarım

https://doi.org/10.5578/kvj.6466

Öz

The purpose of the study was to investigate serum ADA activity and blood lymphocyte and monocyte numbers in the bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex. Twenty five female dogs were involved in the study. Samples from 18 bitches with open cervix CEHpyometra and 7 mid-diestrus bitches were used. The diagnosis of CEHpyometra was based on clinical, ultrasonographical and haematological findings. Mid-diestrus control bitches were diagnosed by vaginal cytologic examination. Blood samples were taken before the operation and tissue samples were collected at the time of surgery from the uteri of 18 adult bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex and of 7 healthy middiestrus dogs presented for ovariohysterectomy for histopathological investigation. Serum ADA activity was measured by spectrophotometrically and determined to be 5.99±0.31 U/l in bitches with CEH-pyometra and 3.50±0.64 U/l in mid-diestrus bitches. Mean serum ADA activity were higher in bitches with CEH-pyometra complex compared with the concentrations in healthy mid-diestrus dogs (p≤0.01). It was concluded that CEH-pyometra complex results in increase in serum ADA activity by cell-mediated immune system and evaluation of serum ADA activity could be an useful parameter for diagnosis and treatment follow-up of this complex

Anahtar Kelimeler

ADA

Kistik Endometriyal Hiperplazi-Pyometra Kompleks Tanısı Konulan Köpeklerde Serum Adenozin Deaminaz Aktivitesinin Belirlenmesi

Öz

Bu çalışmanın amacı, kliniğimize getirilen ve kistik endometriyal hiperplazi (KEH)-pyometra kompleks tanısı konulan köpeklerde serum adenozin deaminaz aktivitesinin belirlenmesi ve sonuçların kan lenfosit ve monosit sayılarıyla ilişkisinin araştırılmasıdır. Çalışmada açık serviks KEHpyometrası bulunan 18 köpek ve diöstrus döneminin ortasında olan 7 sağlıklı köpek olmak üzere toplam 25 köpek kullanıldı. KEH-pyometra tanısı klinik, ultrasonografik ve hematolojik bulgulara dayanılarak konuldu. Kontrol köpeklerinin diöstrusun ortasında olduğu sitolojik muayeneyle belirlendi. Ovaryohisterektomi yapılmadan önce kan örnekleri alındı ve operasyon sonrası histopatolojik incelemeler için doku örnekleri alındı. ADA aktivitesi spektrofotometrik olarak ölçüldü. Serum ADA aktivitesi KEH-pyometralı köpeklerde 5.99±0.31 U/l, diöstrustaki kontrol köpeklerinde ise 3.50±0.64 U/l olarak belirlendi. Ortalama serum ADA aktivitesi (KEH)-pyometra kompleks tanısı konulan köpeklerde sağlıklı köpeklere göre daha yüksek bulundu (p≤0.01). Sonuç olarak, KEH-pyometra kompleksinin köpeklerde hücre aracılı bağışıklık sistemini uyararak serum ADA aktivitesinde yükselmeye neden olduğu ve serum ADA aktivitesinin değerlendirilmesinin bu hastalığın tanısında ve tedavisinin izlenmesinde yararlı bir parametre olabileceği kanısına varılmıştır. ●●● Determination of Serum Adenosine Deaminase Activity in Bitch Diagnosed as Cystic Endometrial Hyperplasia (CEH)-pyometra Complex S U M M A R Y The purpose of the study was to investigate serum ADA activity and blood lymphocyte and monocyte numbers in the bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex. Twenty five female dogs were involved in the study. Samples from 18 bitches with open cervix CEHpyometra and 7 mid-diestrus bitches were used. The diagnosis of CEHpyometra was based on clinical, ultrasonographical and haematological findings. Mid-diestrus control bitches were diagnosed by vaginal cytologic examination. Blood samples were taken before the operation and tissue samples were collected at the time of surgery from the uteri of 18 adult bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex and of 7 healthy middiestrus dogs presented for ovariohysterectomy for histopathological investigation. Serum ADA activity was measured by spectrophotometrically and determined to be 5.99±0.31 U/l in bitches with CEH-pyometra and 3.50±0.64 U/l in mid-diestrus bitches. Mean serum ADA activity were higher in bitches with CEH-pyometra complex compared with the concentrations in healthy mid-diestrus dogs (p≤0.01). It was concluded that CEH-pyometra complex results in increase in serum ADA activity by cell-mediated immune system and evaluation of serum ADA activity could be an useful parameter for diagnosis and treatment follow-up of this complex.

Anahtar Kelimeler

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Kaynakça

• Adams A, Harkness RA. 1976. Adenosine deaminase activity in thymus and other human tissues. Clin Exp Immunol, 26, 647-649.
• Baganha MF, Pego A, Lima MA, Gaspar EV, Cordeiro AR. 1990. Serum and pleural adenosine deaminase. Correlation with lymphocytic populations. Chest, 97, 605-610.
• Da Silva A, França R, Costa M, Paim F, Pimentel V, Schmatz R, Jaques J, Schetinger MR, Mazzantti C, Tonin A, Lopes S. 2013. Adenosine levels in serum and E-ADA activity in blood cells of dogs infected by Rangelia vitalii. J Parasitol, doi: http://dx.doi.org/10.1645/13-176.1
• Dow C. 1957. The cystic hyperplasia-pyometra complex in the bitch. Vet Rec, 69, 1409-1415. Faldyna M, Laznicka A, Toman M. 2001. Immunosuppression in bitches with pyometra. J Small Anim Pract, 42, 5-10.
• Gakis C, Calia G, Naitana A, Pirino D, Serru G. 19 Serum adenosine deaminase activity in HIV positive subjects. A hypothesis on the significance of ADA2. Panminerva Med, 31, 107Gakis C. 1996. Adenosine deaminase (ADA) isoenzymes ADA1 and ADA 2: diagnostic and biological role. Eur Respir J, 9, 632-633.
• Grosjean H, Auxilien, S, Constantinesco F, Siman, C, Corda Y, Becker HF, Foiret D, Morin A, Jin YX, Fournier M, Fourrey JL. 19 Enzymatic conversion of adenosine to inosine and to N1-methylinosine in transfer RNAs. Biochemie, 78, 488-501. Giusti G, Galanti B. Colorimetric method. In: Bergmeyer HU, ed. Methods of enzymatic analysis. Weinheim: Verlag Chemie, 1984, 315-3
• Hirschberger J, Koch S. 1996. Validation of an adenosine deaminase assay and its use in the evaluation of body fluids in dogs. Vet Clin Pathol, 25, 100-104.
• Hirschberger J, Koch S. 1995. Validation of the determination of the activity of adenosine deaminase in the body effusions of cats. Res Vet Sci, 59, 226-229. in women with hyperemesis gravidarum. Clin Chim Acta, 324, 141-145.
• Yoneyama Y, Sawa R, Suzuki S, Otsubo Y, Miura A, Kuwabara Y, Ishino H, Kiyokawa Y, Doi D, Yoneyama K, Kobayashi H, Araki T. 2002b. Serum adenosine deaminase activity in women with pre-eclampsia. Gyn Obst Invest, 54, 164-167.