Determination and Antimicrobial Susceptibility of Microorganisms Isolated from Otitis Externa with Dogs

Yıl 2014, Cilt: 7 Sayı: 1, 27 - 31, 23.07.2014

Ayşe Ebru Borum Göksen Çeçen Gülşah Demir Cengiz Cetin Sezgin Şentürk

https://doi.org/10.5578/kvj.7337

Cited By: 4

Öz

Determination and Antimicrobial Susceptibility of Microorganisms Isolated from Otitis Externa with Dogs In this study, 54 ear swabs taken from suspected of otitis externa with dogs in Uludag University Faculty of Veterinary Medicine. Microorganisms isolated and identified. We determined the susceptibility of bacteriae to antibiotics. Microorganisms isolated from 52 (96.29 %) samples of 54 ear swabs. 12 bacteriae , 5 fungi, and 2 yeast isolated from 52 samples. The microorganisms were isolated in pure culture from (38.46 ) samples and were isolated in mixed culture from (%61.53) samples. Staphylococcus aureus (55.76% ) were most commonly isolated and respectively, Aspergillus fumigatus ( 17.30 % ), Malassezia pachydermatitis ( 17.30 % ) , coagulase -negative Staphylococcus spp. ( 17.30 % ) , Candida spp. ( 11.53% ) ,Epidermophyton spp. ( 9.61% ) , Penicillium spp . (7.69%) , Bacillus spp. (5.76% ) , Citrobacter spp. (5.76% ), Microsporum spp. (5.76% ) , Klebsiella spp. (3.84%) , Streptococcus spp. (3.84%) , Escherichia coli (3.84%) , Micrococcus spp. (3.84%) , Trichophyton spp. (3.84%) , Pseudomonas spp. ( 1.92 % ) , Corynebacterium pyogenes ( 1.92 % ) , Enterobacter spp. ( 1.92 % ) , Acinetobacter spp. ( 1.92 % ) were isolated and identified. We determined Sulfamethoxazole /Trimethoprim and neomycin were the most effective antibiotics. Rifampicin wasfound to be the least effective

Anahtar Kelimeler

Dog Neomycine Otitis externa Staphylococcus aureus Sulfamethoxazole Trimethoprim

Köpeklerde Otitis Externa Vakalarından İzole edilen Mikroorganizmalar ve Antibakteriyel Duyarlılıklarının Belirlenmesi

Öz

Bu çalışmada Uludağ Üniversitesi Veteriner Fakültesine getirilen ve klinik muayenede otitis externa şüpheli 54 köpekten kulak svapları alınmış, mikroorganizmalar izole ve identifiye edilerek bakterilerin antibiyotik duyarlılıklarını belirlemek amaçlandı. Elli dört örnekten 52’sinde (%96.29) üreme görüldü. Elli iki örnekten 12 farklı bakteri, 5 farklı mantar ve 2 farklı maya üremesi tespit edildi. Üremelerin 20’si (%38.46) saf kültür, 32’si ise (%61.53) karışık kültür şeklinde izole edildi. Staphylococcus aureus (%55.76) en fazla izole edilen etken iken, bunu sırasıyla Aspergillus fumigatus (%17.30), Malassezia pachydermatitis (%17.30), Koagulaz negatif Staphylococcus spp. (%17.30), Candida spp. (%11.53), Epidermophyton spp. (%9.61), Penicillium spp. (%7.69), Bacillus spp. (%5.76), Citrobacter spp. (%5.76), Microsporum spp. (%5.76), Klebsiella spp. (%3.84), Streptococcus spp. (%3.84), Escherichia coli (%3.84), Micrococcus spp. (%3.84), Trichophyton spp. (%3.84), Pseudomonas spp. (%1.92), Corynebacterium pyogenes (%1.92), Enterobacter spp. (%1.92), Acinetobacter spp. (%1.92) izledi. İzole edilen bakterilere antibiyotik duyarlılık testi yapıldı ve en etkili iki antibiyotiğin Sulfamethoxazole/Trimethoprim ve Neomisin olduğu belirlendi. En az etkili antibiyotik ise rifampisin olarak saptandı. ●●● S U M M A R Y Determination and Antimicrobial Susceptibility of Microorganisms Isolated from Otitis Externa with Dogs In this study, 54 ear swabs taken from suspected of otitis externa with dogs in Uludag University Faculty of Veterinary Medicine. Microorganisms isolated and identified. We determined the susceptibility of bacteriae to antibiotics. Microorganisms isolated from 52 (96.29 %) samples of 54 ear swabs. 12 bacteriae , 5 fungi, and 2 yeast isolated from 52 samples. The microorganisms were isolated in pure culture from (38.46 ) samples and were isolated in mixed culture from (%61.53) samples. Staphylococcus aureus (55.76% ) were most commonly isolated and respectively, Aspergillus fumigatus ( 17.30 % ), Malassezia pachydermatitis ( 17.30 % ) , coagulase -negative Staphylococcus spp. ( 17.30 % ) , Candida spp. ( 11.53% ) ,Epidermophyton spp. ( 9.61% ) , Penicillium spp . (7.69%) , Bacillus spp. (5.76% ) , Citrobacter spp. (5.76% ), Microsporum spp. (5.76% ) , Klebsiella spp. (3.84%) , Streptococcus spp. (3.84%) , Escherichia coli (3.84%) , Micrococcus spp. (3.84%) , Trichophyton spp. (3.84%) , Pseudomonas spp. ( 1.92 % ) , Corynebacterium pyogenes ( 1.92 % ) , Enterobacter spp. ( 1.92 % ) , Acinetobacter spp. ( 1.92 % ) were isolated and identified. We determined Sulfamethoxazole /Trimethoprim and neomycin were the most effective antibiotics. Rifampicin wasfound to be the least effective.

Anahtar Kelimeler

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Kaynakça

• Blanco JL, Guedeja-Marrón J, Blanco I, García ME. Optimum incubation conditions for the isolation of yeasts from canine otitis externa. Vet Dermatol. 2007; 18(2):120-126.
• Carlotti DN, Laffort DC. Malassezia dermatitis in dog: review and medicale study of 12 cases treated with azole derivatives. Prat Med Chir Anim. 1996; 31 (4): 297-307.
• Girao MD, Prado MR, Brilhante RSN, Cordeiro RA, Monteiro AJ, Sidrim JJC, Rocha MFG. Malassezia pachydermatis isolated from normal and diseased external ear canals in dogs: A comparative analysis. Vet J. 2006;172:544–548.
• Guedeja-Marron I, Blanco lL, Garcia ME. Antimicrobial sensitivity in microorganisms isolated from canine otitis externa. J Vet Med B. 1997; 44: 341.346.
• Guillot J, Gueho E, Lesourd M, Midgley G, Chevrier G, Dupont B. Identification of Malassezia species. A practical approach. J Mycol Med. 1996; 6:103–110.
• Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST . Facultatively Anaerobic Gramnegative Rods (in) Bergeys Manual of Determinative Bacteriology, Ed; Williams and Wilkins, 9th. , East Preston Street, Baltimore, Maryland, USA. 1994; 175-290.
• Jacobsona LS. Diagnosis and medical treatment of otitis externa in the dog and cat. J S Afr Vet Assoc. 2002;73(4):162-70.
• Keskin O, Kökçü L, Akan M. Otitis eksternalı köpeklerden izole edilen mikroorganizmalar ve antibiyotik duyarlılıkları. Ankara Üniv Vet Fak Derg. 1999; 46: 163-168.
• Kiss G, Radvanyi S, Szigeti G. New combination for the therapy of canine otitis externa I Microbiology of Otitis externa. J Small Anim Pract. 1997; 38: 51-55.
• Kuyucuoğlu Y, Sarıtaş ZK. Sağlıklı Köpeklerin Dış Kulak Kanalından İzole Edilen Mikroorganizmalar ve Antibiyotik Duyarlılıkları. Kocatepe Vet J. 2010; 3 (2): 19
• Lilenbaum W, Veras M, Blum E, Souza GN. Antimicrobial susceptibility of staphylococci isolated from otitis externa in dogs. Lett Appl Microbiol. 2000; 31: 42-45.
• Lyskova P, Vydrzalova M, Mazurova J. Identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa. J Vet Med A. 2007; 54: 559-563.
• Martín Barrasa JL, Lupiola Gómez P, González Lama Z, Tejedor Junco MT.. Antibacterial susceptibility patterns of Pseudomonas strains isolated from chronic canine otitis externa. J Vet Med B Infect Dis Vet Public Health. 2000; 47(3):191-196. 31