Kronik Solunum Sistemi Problemleri Olan Keçi Sürülerinde Caprine Arthritis-Encephalitis Virus (CAEV) Enfeksiyonunun Rolünün Araştırılması

Yıl 2014, Cilt: 7 Sayı: 2, 9 - 16, 01.01.2014

Volkan Cenk Özkan Abuzer Acar Sibel Gür

Öz

Caprine Arthritis-Encephalitis Virus (CAEV) Retroviridae familyasının Lentivirus subfamilyasında sınıflandırılmış olup, keçilerde başlıca arthritis, mastitis, intersitisyel pnömoni ve ensefalitise neden olur. Bu çalışmanın amacı normal ve kronik solunum sistemi bozuklukları olan keçi sürülerinde CAEV enfeksiyonu’nun rolünün araştırılmasıdır. Bu amaçla 13 keçi sürüsünden toplam 422 kan serum örneği elde edildi, bunlardan 4 sürüde hafif-orta seviyede kronik solunum sistemi bozuklukları bulunmakta idi. Örneklenen keçilerin yaşları 6 ay ile üzerinde ve çoğunluğu dişilerden oluşmaktaydı. Örnekleme sırasında klinik muayene bulguları ile Body Condition Score (BCS) verileri de elde edilmiştir. Serum örnekleri indirekt ELISA kullanılarak kontrol edildi. Test sonuçlarına göre; klinik olarak normal olan 9 sürünün 4’ünde %1.2 (1/78) ile %6.7 (4/59) arasında değişen oranlarda CAEV yönünden pozitiflik tespit edildi. Kronik solunum sistemi bozuklarının olduğu belirlenen 4 işletmenin tümünde seropozitiflik belirlendi. Buna göre, oranların %2.2 (1/45) ile %12 (3/25) arasında değişmekle birlikte ortalama %6.8’inin (11/160) pozitif olduğu görüldü. BCS değerlerinin aritmetik ortalaması sağlıklı sürülerde 3.1 iken diğer sürülerde 2.7 olduğu tespit edildi. Veriler istatistiksel olarak olarak incelendi ve gruplar arasındaki fark anlamlı bulundu.

Anahtar Kelimeler

CAEV, Keçi, Seroloji, Solunum Sistemi

An investigation for the role of Caprine Arthritis-Encephalitis Virus (CAEV) infection in Goat herds with Chronic Respiratory System Disorders

Öz

Caprine Arthritis-Encephalitis Virus (CAEV) is classified in Lentivirus subfamily in the Retroviridae family and cause to mainly arthritis, mastitis, intersitital pneumonia and encephalitis in goats. Aim of the study to is to investigate the role of CAEV infection in both normal and chronic respiratory system disorders determined goat flocks. For this purpose total of 422 blood serum samples were collected from 13 goat flocks, which 4 of them have low-mild level chronic respiratory disorders. The sampled goats were 6 month and older, the most of them female. Clinical examination and the Body Condition Score (BCS) data have been obtained during sampling. Serum samples were controlled for CAEV using indirect ELISA. According to the test results; out of clinically normal 9 flocks, CAEV positivity was detected in 4 between 1.2% (1/78) and 6.7% (4/59) proportions. Seropositivity was detected in all of the 4 enterprises that have clinical respiratory system disorders; proportions were varied between 2.2% (1/45) and 12% (3/25), average 6.8% (11/160). Arithmetic means of BCS were 3.1 in clinically healthy goat flocks while 2.7 in the other flocks. Obtained data statistically analysed, difference between the groups was found to be significant.

Anahtar Kelimeler

CAEV, Goat, Serology, Respiratory system

Kaynakça

• Adams DS, Oliver RE, Ameghino E, Demartini JC, Verwoerd DW, Houwers DJ, Waghela S, Gorham JR, Hyllseth B, Dawson M, Trigo FC, Mcgire TC. Serological evidence of caprine arthritis-encephalitis infection in eleven of fourteen countries tested. Vet Rec.1984; 115: 493–495.
• Aslantaş O, Özyörük F, Pınar D, Güngör B. Serological survey for caprine arthritis-encephalitis virus in Damascus and Kilis Goats in Hatay, Turkey. Revue Med Vet.2005; 156: 402-404.
• Azkur AK, Gazyağcı S, Aslan ME. Serological and Epidemiological Investigation of Bluetongue, Maedi-Visna and Caprine Arthritis-Encephalitis Viruses in Small Ruminant in Kırıkkale District in Turkey. Kafkas Univ Vet Fak Derg.2011; 17: 803-808.
• Bandeira DA, De Castro RS, Azevedo EO. De Souza Seixas Melo L, De Melo CB. Seroprevalence of caprine arthritis-encephalitis virus in goats in the Cariri region, Paraiba state, Brazil. Vet J.2009; 180: 399-401.
• Benavides J, Garcia-Pariente C, Fuertes M, Ferreras MC, Garcia-Marin JF, Juste RA, PerezV.Maedi-visna: the meningoencephalitis in naturally occurring cases. J Comp Pathol.2009; 140 (1): 1–11.
• Burgu İ, Akça Y, Alkan F, Özkul A, Karaoğlu T, Çabalar M. Antibody prevalence of Caprine arthritis encephalitis virus (CAEV) in goats in Turkey. DTW.1994;10: 390-391.
• Chebloune Y, Karr BM, Singh DK, Narayan O. Maedi-visna and caprine arthritis encephalitis viruses: classical animal models of persistent lentiviral infection. In Persistent viral infections. Edited by Ahmed R, Chen ISY. Chichester: John Wiley & Sons Ltd. 1998;pp. 347-361.
• Cheevers WP, Knowles DP, Mcguire TC, Cunningham DR, Adams DS, Gorham JR. Chronic disease in goats orally infected with two isolates of the caprine arthritis-encephalitis lentivirus. Lab Invest.1988; 58: 510 –517.
• Cork LC, Hadlow WJ, Crawford TB, Gorham JR,PiperRC.Infectionsleukoencephalomyelitis of young goats. J Inf Dis.1974; 129: 134–141.
• Cork LC, Narayan O. The pathogenesis of viral leukoencephalomyelitis –arthritis of goats. I. Persistent viral infection with progressive pathologic changes. Lab Invest.1980; 42(6):596-602.
• Crawford TB, Adams DS, Cheevers W, Cork LC. Chronic arthritis in goats caused by a retrovirus. Science.1980; 207:997-999.
• Crawford TB, Adams DS, Cheevers WP, Cork LC. Chronic arthritis in goats caused by a Retrovirus. Science.1980;207: 977-999.
• Damassa AJ, Wakenell PS, Brooks DL. Mycoplasmas of goats and sheep. J Vet Diagn Invest.1992;4:101-113. East NE, Rowe JD, Madewell BR. Serologic prevalence of caprine arthritis encephalitis virus in goats on California dairies. J Am Vet Med Assoc.1987;190:182.
• Greenwood PL. Effects of caprine arthritis-encephalitis virus on productivity and health of dairy goats in New-South-Wales, Australia. Prev Vet Med.1995;22: 71–87.
• Kinde H, Damassa AJ, Wakenell PS, Petty R. Mycoplasma infection in a commercial goat dairy caused by Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (caprine biotype). Vet Diagn Invest.1994, 6: 423-427.
• Leroux C, Chastang J, Greenland T, Mornex JF. Genomic heterogeneity of small ruminant lentiviruses: existence of heterogeneous populations in sheep and of the same lentiviral genotypes in sheep and goats. Arch Virol.1997;142(6):1125-37.
• Luengo C, Sanchez A, Corrales JC, Fernandez C, Contreras A. Influence of intramammary infection and non-infection factors on somatic cell counts in dairy goats. J Dairy Res.2004; 71:169–174.
• Malher X, Seegers H, Beaudeau F. Culling and mortality in large dairy goat herds managed under intensive conditions in western France. Livest Prod Sci.2001; 71:75–86.
• Nord K, Ådnøy TA.Effects of infection by caprine arthritis-encephalitis virus on milk production of goats. J Dairy Sci.1997;80:2391–2397.
• Peretz G, Asso J, Devillechaise P. Le CAEV : Revue des connaissances actuelles et conséquences pratiques. Revue Méd Vét.1993; 144: 93-98.
• Pisoni G, Quasso A, Moroni P. Phylogenetic analysis of small-ruminant lentivitus subtype B1 in mixed flocks: Evidence for natural transmission from goats to sheep. Virology.2005; 339: 147-152.
• Rowe JD, East NE. Risk factors for transmission and meth-ods for control of caprine arthritis-encephalitis virus in-fection. Vet Clin North Am Food Anim Pract.1997; 13:35-53.
• Sanchez A, Contreras A, Corrales, JC, Marco JC. Relationships between infection with caprine arthritis encephalitis virus, intramammary bacterial infection and somatic cell counts in dairy goats. Vet Rec.2001; 148: 711-714.
• Shah C, Boni J, Huder JB, Vogt HR, Muhlherr J, Zanoni R, Mıserez R, Lutz H, Schupbach J. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology.2004a; 319: 12-26.
• Shah C, Huder JB, Boni J, Schonmann M, Muhlherr J, Lutz H, Schupbach J. Direct evidence for natural transmission of small-ruminant lentiviruses of subtype A4 from goats to sheep and vice versa. Virology.2004b;78: 7518-7522.
• Turin L, Pisoni G, Giannino ML, Antonini M, Rosati S, Ruffo G, Moroni P. 2005. Correlation between milk parameters in CAEV seropositive and negative primiparous goats during an eradication program in Italian farm. Small Rum Res, 57: 73-79.
• Türkiye istatistik kurumu (tuik). 05.01.2012. http://www.tuik.gov.tr/PreTablo.do?tb_id=46&ust_id=13 Yavru S, Şimşek A, Kale M, Bulut, O. 2002. Konya bölgesinde keçi Caprine arthritis encephalitis virusu (CAEV) enfeksiyonunun AGID ve ELISA testleriyle serolojik olarak araştırılması. V. Uluslarası Veteriner Mikrobiyoloji Kongresi, 24-26 Eylül 2002, Konya.