Araştırma Makalesi
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Sıçanlarda Bekletilmiş, Distile ve Deiyonize Kaplıca Suyu Tüketilmesinin Oksidan Antioksidan Denge ve Bazı Biyokimyasal Parametreler Üzerine Etkisinin Belirlenmesi

Yıl 2016, Cilt 9, Sayı 3, 171 - 178, 01.09.2016

Öz

Bu çalışmada, Afyonkarahisar ilinin Gazlıgöl bölgesinden alınan kaplıca suyu ve bu suyun bekletilerek, distile ve deiyonize edilerek tüketilmesinin oksidan-antioksidan denge ve bazı biyokimyasal parametreler ile karaciğer ve böbrek dokusuna etkilerinin belirlenmesi amaçlanmıştır. Çalışmada her grupta 6 sıçan yer almıştır. 30 gün boyunca, deney grubundaki sıçanlara taze, bekletilmiş, deiyonize ve distile edilmiş kaplıca suyu, kontrol grubundakilere ise normal su verilmiştir. Deneme sonunda sıçanlardan kan numuneleri ile karaciğer, böbrek ve kalp doku örnekleri alınmıştır.Hemoglobin değeri bekletilmiş kaplıca suyu tüketen sıçanlarda taze ve deiyonize tüketen sıçanlara göre önemli oranda düşük olduğu gözlenmiştir(p<0,05). Distile edilmiş kaplıca suyu tüketen sıçanlarda Çinko değerinde diğer gruplara göre anlamlı (p<0,05) bir düşüş oluşmuştur. Karaciğer ve böbrek dokusu MDA, GSH ve SOD düzeyleri bakımından gruplar arasında anlamlı bir değişim gözlenmemiştir. Buna karşın, kalp dokusunda MDA düzeylerinin bekletilmiş ve deiyonize kaplıca suyu tüketen sıçanlarda diğer gruptakilere göre daha yüksek olduğu (p<0,01), GSH ile SOD düzeylerinin ise değişmediği saptanmıştır. Karaciğer ve böbrek dokusu hispatolojik incelemesinde birtakım patolojik değişiklikler tespit edilmiştir. Sonuç olarak çalışmada kullanılan kaplıca suyunun organizmada olumlu bir etki yaratmadığı kanısına varılmıştır.

Kaynakça

  • Albertini M. C, Dachà M, Teodori L, Conti M E. Drinking mineral waters: biochemical effects and health implications – The State-of-The-Art. Int. J. Environmental Health. 2007; 1(1): 153–69.
  • Benedetti S, Benvenuti F, Nappi G, Fortunati NA, Marino L, Aureli T, De Luca S, Pagliarani S, Canestrari F. Antioxidative effects of sulfurous mineral water: protection against lipid and protein oxidation. European Journal Of Clinical Nutrition. 2009; 63(1):106.
  • Beutler E. “Red Cell Metabolism. A Manual of Biochemical Methods,” 3rd. Ed: Grune and Stratton.1984. Costantino M, Giampaolo C, Filippelli A. Effects of Drinking Spa Therapy on Oxidative Stres. Clin Ter. 2012; 163(1):e13-7.
  • Çevik C, Eryavuz A, Ulutaş E, Küçükkurt İ, Bülbül A, Demirel HH, Taşkıran N, Aslan R. Determination of Influences of Long Time Consumption of Thermal Water and Thermal Water With Vitamin C for Oxidant-Antioxidant Balance and Some Biochemical Parameters in Rats. KVD. 2011; 4(2):23-22.
  • Draper HH, Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186:421-431.
  • Erer S. The hıstorıcal development and the importance of Bursa Kükürtlü baths from the points of view of the Turkish History of Medicine. J Med Ethics. 2004; 12:189-195.
  • Granados AMP, Carreteroa SN, Schoppena S, Vaquer MP. Reduction in cardiovascular risk by sodium-bicarbonated mineral water in moderately hypercholesterolemic young adults. Journal of Nutritional Biochemistry. 2010; 21: 948–953.
  • Karagülle MZ, Karagülle M. Balneotherapy and spa therapy in Elderly. Turkish Journal Of Geriatrics. 2000; 3: 120.
  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-361.
  • Luna LG. Manual of histologic staining methods of the armed forces İnstitute of Pathology, 3rd Ed., mcgraw-Hill Book Co., New York, 1968; pp: 32-46.
  • Mergen H, Mergen EB, Erdoğmuş AB. Geothermal Energy and Balneotherapy Applications. FTR Bil Der J PMR. 2006; 9(3):108-113.
  • Nasuti C, Gabbianelli R, Cantalamessa F. Giancarlo Falcioni erythrocyte plasma membrane perturbations in rats fed a cholesterol-rich diet: effect of drinking sulphurous mineral water. Ann Nutr Metab. 2005; 49:9–15.
  • Passariello A, DI Costanzo M, Terrin G, Iannotti A, Buono P, Balestrieri U, Balestrieri G, Ascione E, Pedata M, Canani FB, Canani RB . Crenotherapy Modulates The Expression Of Proinflammatory Cytokines And İmmunoregulatory Peptides İn Nasal Secretions Of Children With Chronic Rhinosinusitis. Am J Rhinol Allergy. 2012; 26(1):e15-9.
  • Schoppen S, Granados AMP, Carbajal A, Oubiña P, Sánchez Muniz FJ, Gómez-Gerique Ja, Pilar Vaquero M. A sodium-rich carbonated mineral water reduces cardiovascular risk in postmenopausal women. J Nutr. 2004; 134:1058–1063.
  • Sharp PE, La Regina MC. The Laboratory Rat. 1998; pp:29,50.
  • Sözbilir BN, Bayşu N. Biochemistry. 2th edition, Ankara.2000; pp: 228, 361-363.
  • Sun Y, Oberley LW, Li Y . A simple method for clinical assay of superoxide dismutase. Clin Chem, 1988; 34: 497-500.
  • Vaccarezza M, Vitale M. Crenotherapy: a neglected resource for human health now re-emerging on sound scientific concepts. Int J Biometeorol, 2010; 54:491–493.
  • İssi M., Gül Y. Blood and Sampling Techniques in Animal Experiments. JCAM. Online ISSN : 1309-2014. (www.jcam.com.tr/files/KATD-604.pdf. Accessien date: 17.12.2015)
  • World Health Organization. Health Risks From Drinking Deminaralized Water. http://www.who.int/water_sanitation_health/dwq/nutrientschap12.pdf; Accessien date: 17.12.2015.

Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats

Yıl 2016, Cilt 9, Sayı 3, 171 - 178, 01.09.2016

Öz

The objective of this research is to determine the effects of the consumption of the bottled, distilled and deionized versions of thermal water taken from the Gazlıgöl district in the city of Afyonkarahisar on the oxidant – antioxidant balance and on certain biochemical parameters, along with possible effects on liver and kidney tissue. The research involved 6 rats in each group. The experiment group was given fresh, bottled, deionized and distilled thermal water for 30 days whereas the control group was given normal water. At the end of the experiment, rats’ blood samples as well as liver, kidney and heart tissue samples were taken. The research determined that the hemoglobin level in rats that consumed bottled thermal water is considerably lower than those that consumed fresh and deionized water (p<0,05). The zinc level was meaningfully lower in rats that consumed distilled water than those in the other groups (p<0,05). There was no meaningful difference among the groups in terms of the liver and kidney tissues’ MDA, GSH and SOD levels. Nevertheless, it was observed that the heart tissues’ MDA level was higher in rats that consumed bottled and deionized water than those in other groups (p<0,01) whereas the GSH and SOD levels did not change. The histopathological examination of liver and kidney tissue showed certain pathological changes.The conclusion of the research is that thermal water does not have a positive effect on the organism.

Kaynakça

  • Albertini M. C, Dachà M, Teodori L, Conti M E. Drinking mineral waters: biochemical effects and health implications – The State-of-The-Art. Int. J. Environmental Health. 2007; 1(1): 153–69.
  • Benedetti S, Benvenuti F, Nappi G, Fortunati NA, Marino L, Aureli T, De Luca S, Pagliarani S, Canestrari F. Antioxidative effects of sulfurous mineral water: protection against lipid and protein oxidation. European Journal Of Clinical Nutrition. 2009; 63(1):106.
  • Beutler E. “Red Cell Metabolism. A Manual of Biochemical Methods,” 3rd. Ed: Grune and Stratton.1984. Costantino M, Giampaolo C, Filippelli A. Effects of Drinking Spa Therapy on Oxidative Stres. Clin Ter. 2012; 163(1):e13-7.
  • Çevik C, Eryavuz A, Ulutaş E, Küçükkurt İ, Bülbül A, Demirel HH, Taşkıran N, Aslan R. Determination of Influences of Long Time Consumption of Thermal Water and Thermal Water With Vitamin C for Oxidant-Antioxidant Balance and Some Biochemical Parameters in Rats. KVD. 2011; 4(2):23-22.
  • Draper HH, Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186:421-431.
  • Erer S. The hıstorıcal development and the importance of Bursa Kükürtlü baths from the points of view of the Turkish History of Medicine. J Med Ethics. 2004; 12:189-195.
  • Granados AMP, Carreteroa SN, Schoppena S, Vaquer MP. Reduction in cardiovascular risk by sodium-bicarbonated mineral water in moderately hypercholesterolemic young adults. Journal of Nutritional Biochemistry. 2010; 21: 948–953.
  • Karagülle MZ, Karagülle M. Balneotherapy and spa therapy in Elderly. Turkish Journal Of Geriatrics. 2000; 3: 120.
  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-361.
  • Luna LG. Manual of histologic staining methods of the armed forces İnstitute of Pathology, 3rd Ed., mcgraw-Hill Book Co., New York, 1968; pp: 32-46.
  • Mergen H, Mergen EB, Erdoğmuş AB. Geothermal Energy and Balneotherapy Applications. FTR Bil Der J PMR. 2006; 9(3):108-113.
  • Nasuti C, Gabbianelli R, Cantalamessa F. Giancarlo Falcioni erythrocyte plasma membrane perturbations in rats fed a cholesterol-rich diet: effect of drinking sulphurous mineral water. Ann Nutr Metab. 2005; 49:9–15.
  • Passariello A, DI Costanzo M, Terrin G, Iannotti A, Buono P, Balestrieri U, Balestrieri G, Ascione E, Pedata M, Canani FB, Canani RB . Crenotherapy Modulates The Expression Of Proinflammatory Cytokines And İmmunoregulatory Peptides İn Nasal Secretions Of Children With Chronic Rhinosinusitis. Am J Rhinol Allergy. 2012; 26(1):e15-9.
  • Schoppen S, Granados AMP, Carbajal A, Oubiña P, Sánchez Muniz FJ, Gómez-Gerique Ja, Pilar Vaquero M. A sodium-rich carbonated mineral water reduces cardiovascular risk in postmenopausal women. J Nutr. 2004; 134:1058–1063.
  • Sharp PE, La Regina MC. The Laboratory Rat. 1998; pp:29,50.
  • Sözbilir BN, Bayşu N. Biochemistry. 2th edition, Ankara.2000; pp: 228, 361-363.
  • Sun Y, Oberley LW, Li Y . A simple method for clinical assay of superoxide dismutase. Clin Chem, 1988; 34: 497-500.
  • Vaccarezza M, Vitale M. Crenotherapy: a neglected resource for human health now re-emerging on sound scientific concepts. Int J Biometeorol, 2010; 54:491–493.
  • İssi M., Gül Y. Blood and Sampling Techniques in Animal Experiments. JCAM. Online ISSN : 1309-2014. (www.jcam.com.tr/files/KATD-604.pdf. Accessien date: 17.12.2015)
  • World Health Organization. Health Risks From Drinking Deminaralized Water. http://www.who.int/water_sanitation_health/dwq/nutrientschap12.pdf; Accessien date: 17.12.2015.

Ayrıntılar

Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Cahide ÇEVİK Bu kişi benim


Elmas ULUTAŞ Bu kişi benim


Hasan Hüseyin DEMİREL Bu kişi benim


Sinan İNCE Bu kişi benim


Abdullah ERYAVUZ> (Sorumlu Yazar)

0000-0001-8602-2400

Yayımlanma Tarihi 1 Eylül 2016
Yayınlandığı Sayı Yıl 2016, Cilt 9, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { kvj370295, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2016}, volume = {9}, number = {3}, pages = {171 - 178}, title = {Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats}, key = {cite}, author = {Çevik, Cahide and Ulutaş, Elmas and Demirel, Hasan Hüseyin and İnce, Sinan and Eryavuz, Abdullah} }
APA Çevik, C. , Ulutaş, E. , Demirel, H. H. , İnce, S. & Eryavuz, A. (2016). Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats . Kocatepe Veterinary Journal , 9 (3) , 171-178 . Retrieved from https://dergipark.org.tr/tr/pub/kvj/issue/32994/370295
MLA Çevik, C. , Ulutaş, E. , Demirel, H. H. , İnce, S. , Eryavuz, A. "Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats" . Kocatepe Veterinary Journal 9 (2016 ): 171-178 <https://dergipark.org.tr/tr/pub/kvj/issue/32994/370295>
Chicago Çevik, C. , Ulutaş, E. , Demirel, H. H. , İnce, S. , Eryavuz, A. "Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats". Kocatepe Veterinary Journal 9 (2016 ): 171-178
RIS TY - JOUR T1 - Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats AU - Cahide Çevik , Elmas Ulutaş , Hasan Hüseyin Demirel , Sinan İnce , Abdullah Eryavuz Y1 - 2016 PY - 2016 N1 - DO - T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 171 EP - 178 VL - 9 IS - 3 SN - 1308-1594-2147-6853 M3 - UR - Y2 - 2016 ER -
EndNote %0 Kocatepe Veteriner Dergisi Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats %A Cahide Çevik , Elmas Ulutaş , Hasan Hüseyin Demirel , Sinan İnce , Abdullah Eryavuz %T Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats %D 2016 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 9 %N 3 %R %U
ISNAD Çevik, Cahide , Ulutaş, Elmas , Demirel, Hasan Hüseyin , İnce, Sinan , Eryavuz, Abdullah . "Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats". Kocatepe Veterinary Journal 9 / 3 (Eylül 2016): 171-178 .
AMA Çevik C. , Ulutaş E. , Demirel H. H. , İnce S. , Eryavuz A. Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats. Kocatepe Veterinary Journal. 2016; 9(3): 171-178.
Vancouver Çevik C. , Ulutaş E. , Demirel H. H. , İnce S. , Eryavuz A. Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats. Kocatepe Veterinary Journal. 2016; 9(3): 171-178.
IEEE C. Çevik , E. Ulutaş , H. H. Demirel , S. İnce ve A. Eryavuz , "Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats", Kocatepe Veterinary Journal, c. 9, sayı. 3, ss. 171-178, Eyl. 2016

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