Yıl 2017, Cilt 10 , Sayı 3, Sayfalar 172 - 179 2017-03-01

Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues
Ratlarda Gentamisin İle İndüklenmiş Oksidatif Streste Borun Muhtemel Koruyucu Etkisinin Dokularda Araştırılması#

İsmail KÜÇÜKKURT [1] , Damla ARSLAN ACARÖZ [2] , Hasan Hüseyin DEMİREL [3] , Sinan İNCE [4] , Abdullah ERYAVUZ [5]


The objective of this study was to determine the protective effects of boron on gentamicin induced toxicity in rats. Rats were divided into eight experimental groups containing seven animals in each group. Experimental groups were as follows; control group (fed without B), gentamicin group (100 mg/kg, i.p.), B-5 group (5 mg/kg B, i.p.), B-10 group (10 mg/kg B, i.p.), B-20 group (20 mg/kg B, i.p.), B-5 + gentamicin group (5 mg/kg B and 100 mg/kg gentamicin, i.p.), B-10 + gentamicin group (10 mg/kg B and 100 mg/kg gentamicin, i.p.), and B-20 + gentamicin group (20 mg/kg B and 100 mg/kg gentamicin, i.p.). B was given to rats before four days. Gentamicin was given to rats on the fourth day and finished on the twelfth day. Also, administration of B was completed the fourteenth day. End of the experiment, liver, hearth, brain, lung, and testis tissues were collected from animals under anaesthesia. Administration of gentamicin increased malondialdehyde levels whereas decreased reduce glutathione levels, superoxide dismutase and catalase activities in the liver. Histopathologically, damages were detected in the tissues of gentamicin group. However, treatment of boron resulted in a reversal of gentamicin-induced damages.
Çalışmada gentamisin maruziyeti ile toksisite oluşturulan ratlarda borun (B) muhtemel koruyucu etkisinin belirlenmesi amaçlandı. Bu amaçla Wistar albino 56 erkek rat kullanıldı. Her grupta 7 hayvan olacak şekilde, kontrol, gentamisin (100 mg/kg i.p.), B-5 (5 mg/kg B, i.p.), B-10 (10 mg/kg B, i.p.), B-20 (20 mg/kg B, i.p.), B-5 + gentamisin (5 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-10 + gentamisin (10 mg/kg B ve 100 mg/kg gentamisin, i.p.), B-20 + gentamisin (20 mg/kg B ve 100 mg/kg gentamisin, i.p.) olarak 8 gruba ayrıldı. Ratlara B uygulaması gentamisin enjeksiyonundan 4 gün önce verilmeye başlandı. Gentamisin uygulamasına 4. gün başlandı ve 12. gün bu uygulama sonlandırıldı. Gentamisin uygulamasının bitişinden itibaren hayvanlara 2 gün daha B verildi. Uygulama sonunda hayvanlardan anestezi altında karaciğer, kalp, beyin, akciğer ve testis dokuları alındı. Gentamisinin karaciğer dokusunda malondialdehid düzeylerini arttırdığı, buna karşın redükte glutatyon düzeylerini, superoksid dismutaz ve katalaz aktivitelerini azalttığı tespit edildi. Histopatolojik incelemede gentamisinin dokularda hasar oluşturduğu gözlendi. Buna karşın, B uygulamasının gentamisin ile indüklenen hasarı azalttığı tespit edildi.
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Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Yazar: İsmail KÜÇÜKKURT

Yazar: Damla ARSLAN ACARÖZ (Sorumlu Yazar)

Yazar: Hasan Hüseyin DEMİREL

Yazar: Sinan İNCE

Yazar: Abdullah ERYAVUZ

Tarihler

Yayımlanma Tarihi : 1 Mart 2017

Bibtex @araştırma makalesi { kvj368878, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2017}, volume = {10}, pages = {172 - 179}, doi = {}, title = {Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues}, key = {cite}, author = {KÜÇÜKKURT, İsmail and ARSLAN ACARÖZ, Damla and DEMİREL, Hasan Hüseyin and İNCE, Sinan and ERYAVUZ, Abdullah} }
APA KÜÇÜKKURT, İ , ARSLAN ACARÖZ, D , DEMİREL, H , İNCE, S , ERYAVUZ, A . (2017). Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues. Kocatepe Veterinary Journal , 10 (3) , 172-179 . Retrieved from https://dergipark.org.tr/tr/pub/kvj/issue/32998/368878
MLA KÜÇÜKKURT, İ , ARSLAN ACARÖZ, D , DEMİREL, H , İNCE, S , ERYAVUZ, A . "Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues". Kocatepe Veterinary Journal 10 (2017 ): 172-179 <https://dergipark.org.tr/tr/pub/kvj/issue/32998/368878>
Chicago KÜÇÜKKURT, İ , ARSLAN ACARÖZ, D , DEMİREL, H , İNCE, S , ERYAVUZ, A . "Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues". Kocatepe Veterinary Journal 10 (2017 ): 172-179
RIS TY - JOUR T1 - Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues AU - İsmail KÜÇÜKKURT , Damla ARSLAN ACARÖZ , Hasan Hüseyin DEMİREL , Sinan İNCE , Abdullah ERYAVUZ Y1 - 2017 PY - 2017 N1 - DO - T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 172 EP - 179 VL - 10 IS - 3 SN - 1308-1594-2147-6853 M3 - UR - Y2 - 2017 ER -
EndNote %0 Kocatepe Veteriner Dergisi Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues %A İsmail KÜÇÜKKURT , Damla ARSLAN ACARÖZ , Hasan Hüseyin DEMİREL , Sinan İNCE , Abdullah ERYAVUZ %T Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues %D 2017 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 10 %N 3 %R %U
ISNAD KÜÇÜKKURT, İsmail , ARSLAN ACARÖZ, Damla , DEMİREL, Hasan Hüseyin , İNCE, Sinan , ERYAVUZ, Abdullah . "Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues". Kocatepe Veterinary Journal 10 / 3 (Mart 2017): 172-179 .
AMA KÜÇÜKKURT İ , ARSLAN ACARÖZ D , DEMİREL H , İNCE S , ERYAVUZ A . Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues. Kocatepe Veterinary Journal. 2017; 10(3): 172-179.
Vancouver KÜÇÜKKURT İ , ARSLAN ACARÖZ D , DEMİREL H , İNCE S , ERYAVUZ A . Potential Protective Effect of Boron Against Gentamicin-Induced Oxidative Stress on Rat Tissues. Kocatepe Veterinary Journal. 2017; 10(3): 179-172.