Yıl 2019, Cilt 12 , Sayı 3, Sayfalar 264 - 267 2019-09-30

Melaminin İnsan Kolon Adenokarsinom Hücreleri Üzerindeki Anti-Proliferatif Etkisi
Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells

Hidayet TUTUN [1]


Melamin, öncelikle plastik dengeleyici ve yangın geciktirici olarak kullanılan organik bir kimyasaldır. Hayvansal yem ve süt ürünlerinin protein içeriğini suni şekilde yükseltmek için kullanılmasıyla bebeklerde böbrek hastalıklarına ve hayvan ölümlerine yol açmıştır. Bu çalışmada, melaminin insan kolon adenokarsinom hücrelerinin (Caco-2) proliferasyonu üzerine etkileri araştırılmıştır. Farklı konsantrasyonlarda çözülmüş melamin Caco-2 hücreleri ile 24 saat inkübe edildi ve MTT testi kullanılarak anti-proliferatif potansiyeli test edildi. Melaminin yarı maksimal inhibisyon konsantrasyonu (IC50), doz-cevap eğrisinden hesaplandı ve hücrelerde 14636 µg/L olarak bulundu. Sonuçlar doza bağımlı olarak melaminin Caco-2 hücrelerinin proliferasyonunu azalttığını ve Caco-2 hücrelerine karşı anti-proliferatif etkiye sahip olduğunu göstermiştir.

Melamine is an organic chemical used primarily as a plastics stabilizer and fire retardant. Also, it was used to fraudulently elevate the protein content of animal feed and dairy products, which led to infant kidney diseases and pet deaths. In this study, melamine was studied for its effects on growth in the human colon adenocarcinoma cells (Caco-2). Different concentrations of medium-dissolved melamine were incubated for 24 h with Caco-2 cells and tested for its anti-proliferative potential using MTT assay. Half-maximal inhibitory concentration (IC50) of melamine was calculated from dose-response curve and found to be 14636 µg/L in the cells. The results demonstrated that the dose dependent melamine decreased cell proliferation and direct anti-proliferative effect in Caco-2 cells. 

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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Orcid: 0000-0001-9512-8637
Yazar: Hidayet TUTUN (Sorumlu Yazar)
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { kvj576695, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2019}, volume = {12}, pages = {264 - 267}, doi = {10.30607/kvj.576695}, title = {Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells}, key = {cite}, author = {TUTUN, Hidayet} }
APA TUTUN, H . (2019). Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells. Kocatepe Veterinary Journal , 12 (3) , 264-267 . DOI: 10.30607/kvj.576695
MLA TUTUN, H . "Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells". Kocatepe Veterinary Journal 12 (2019 ): 264-267 <https://dergipark.org.tr/tr/pub/kvj/issue/45621/576695>
Chicago TUTUN, H . "Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells". Kocatepe Veterinary Journal 12 (2019 ): 264-267
RIS TY - JOUR T1 - Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells AU - Hidayet TUTUN Y1 - 2019 PY - 2019 N1 - doi: 10.30607/kvj.576695 DO - 10.30607/kvj.576695 T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 264 EP - 267 VL - 12 IS - 3 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.576695 UR - https://doi.org/10.30607/kvj.576695 Y2 - 2019 ER -
EndNote %0 Kocatepe Veteriner Dergisi Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells %A Hidayet TUTUN %T Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells %D 2019 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 12 %N 3 %R doi: 10.30607/kvj.576695 %U 10.30607/kvj.576695
ISNAD TUTUN, Hidayet . "Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells". Kocatepe Veterinary Journal 12 / 3 (Eylül 2019): 264-267 . https://doi.org/10.30607/kvj.576695
AMA TUTUN H . Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells. Kocatepe Veterinary Journal. 2019; 12(3): 264-267.
Vancouver TUTUN H . Anti-Proliferative Effect of Melamine on Human Colon Adenocarcinoma Cells. Kocatepe Veterinary Journal. 2019; 12(3): 267-264.