Yıl 2019, Cilt 12 , Sayı 3, Sayfalar 336 - 342 2019-09-30

Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining
Sığır Yağ Dokusundan İzole Edilen Mezenkimal Kök Hücrelerden Farklılaştırılan Nörosferlerde Sox2, ß-III Tubulin ve Nestin Ekspresyonlarının İmmunfloresan Boyama Yöntemi ile İncelenmesi

Özlem ÖZDEN AKKAYA [1] , Tayfun DİKMEN [2] , Shah NAWAZ [3]


Stem cell therapy can be an advantageous tool for non treatable diseases and has become a theme research for a better solution to many neurodegenerative cases. Despite of being very common in pet animals and equines in the field of veterinary medicine; stem cell based practises in bovines yet remain very limited. In the following study, Bovine adipose derived stem cells (BASCs) were isolated and differentiated into neurospheres. The potential expressions of neuron-specific markers β-III Tubulin, Nestin and Sox2 were demonstrated by immunofluorescence staining on these neurospheres. The outcomes of the study may provide a better understanding to the neurogenic potential of BASCs and can be a footstep for developing new therapeutical approaches for neurodegenerative diseases and syndroms in bovines.

Kök hücre tedavisi, pek çok tedavisi olmayan hastalıkta olduğu gibi çoğu nörodejeneratif hastalık ve sendromda da daha iyi tedavi protokollerinin geliştirilmesi için önemli bir çalışma alanı haline gelmiştir. Kök hücrelerin klinik kullanımı pet hayvanları ve atlarda her ne kadar yaygınlaşmaya başlasa da sığırlarda kök hücre kullanımı hala oldukça sınırlıdır. Bu çalışmada sığır yağ dokusu kaynaklı mezenkimal kök hücreler izole edilmiş ve bu hücrelerden nörosfereldesi gerçekleştirilmiştir. Elde edilen nörosferlerin nöronspresifik belirteçler olan ß-III Tubulin, Nestin ve Sox2 ekspresyonları immunfloresan boyama tekniği ile değerlendirilmiştir. Çalışmadan elde edilecek sonuçlar sığır yağ dokusu kaynaklı mezenkimal kök hücrelerin nörojenik potansiyelinin daha iyi anlaşılması açısından önemli olmakla birlikte bu sonuçlar sığırlarda nörojenik defektlerde kullanılabilecek potansiyel tedavi yaklaşımlarının geliştirilmesi hususunda da yol gösterici olacaktır. 

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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Orcid: 0000-0001-6372-9155
Yazar: Özlem ÖZDEN AKKAYA (Sorumlu Yazar)
Kurum: Afyon Kocatepe Universtiy, Faculty of Veterinary Medicine, Department of Histology and Embryology
Ülke: Turkey


Orcid: 0000-0003-4470-7465
Yazar: Tayfun DİKMEN
Kurum: Afyon Kocatepe Universtiy, Faculty of Veterinary Medicine, Department of Histology and Embryology
Ülke: Turkey


Orcid: 0000-0001-5468-8267
Yazar: Shah NAWAZ
Kurum: Afyon Kocatepe Universtiy, Faculty of Veterinary Medicine, Department of Histology and Embryology
Ülke: Pakistan


Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { kvj594550, journal = {Kocatepe Veterinary Journal}, issn = {1308-1594}, eissn = {2147-6853}, address = {}, publisher = {Afyon Kocatepe Üniversitesi}, year = {2019}, volume = {12}, pages = {336 - 342}, doi = {10.30607/kvj.594550}, title = {Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining}, key = {cite}, author = {ÖZDEN AKKAYA, Özlem and DİKMEN, Tayfun and NAWAZ, Shah} }
APA ÖZDEN AKKAYA, Ö , DİKMEN, T , NAWAZ, S . (2019). Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining. Kocatepe Veterinary Journal , 12 (3) , 336-342 . DOI: 10.30607/kvj.594550
MLA ÖZDEN AKKAYA, Ö , DİKMEN, T , NAWAZ, S . "Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining". Kocatepe Veterinary Journal 12 (2019 ): 336-342 <https://dergipark.org.tr/tr/pub/kvj/issue/45621/594550>
Chicago ÖZDEN AKKAYA, Ö , DİKMEN, T , NAWAZ, S . "Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining". Kocatepe Veterinary Journal 12 (2019 ): 336-342
RIS TY - JOUR T1 - Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining AU - Özlem ÖZDEN AKKAYA , Tayfun DİKMEN , Shah NAWAZ Y1 - 2019 PY - 2019 N1 - doi: 10.30607/kvj.594550 DO - 10.30607/kvj.594550 T2 - Kocatepe Veterinary Journal JF - Journal JO - JOR SP - 336 EP - 342 VL - 12 IS - 3 SN - 1308-1594-2147-6853 M3 - doi: 10.30607/kvj.594550 UR - https://doi.org/10.30607/kvj.594550 Y2 - 2019 ER -
EndNote %0 Kocatepe Veteriner Dergisi Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining %A Özlem ÖZDEN AKKAYA , Tayfun DİKMEN , Shah NAWAZ %T Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining %D 2019 %J Kocatepe Veterinary Journal %P 1308-1594-2147-6853 %V 12 %N 3 %R doi: 10.30607/kvj.594550 %U 10.30607/kvj.594550
ISNAD ÖZDEN AKKAYA, Özlem , DİKMEN, Tayfun , NAWAZ, Shah . "Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining". Kocatepe Veterinary Journal 12 / 3 (Eylül 2019): 336-342 . https://doi.org/10.30607/kvj.594550
AMA ÖZDEN AKKAYA Ö , DİKMEN T , NAWAZ S . Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining. Kocatepe Veterinary Journal. 2019; 12(3): 336-342.
Vancouver ÖZDEN AKKAYA Ö , DİKMEN T , NAWAZ S . Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining. Kocatepe Veterinary Journal. 2019; 12(3): 342-336.