At Yağ Dokusu Kaynaklı Mezenkimal Kök Hücrelerin İzolasyonu ve Çoklu Farklılaştırması

Yıl 2020, Cilt 13, Sayı 3, 304 - 312, 30.09.2020

Asm Golam KIBRIA Shah NAWAZ Tayfun DİKMEN Özlem ÖZDEN AKKAYA Artay YAĞCI

https://doi.org/10.30607/kvj.751139

Öz

Mezenkimal kök hücreler kendilerini yenileyebilme ve sınırsız bölünebilme kabiliyetlerinin vasıtasıyla rejeneratif tıp alanında etkili bir kaynak haline gelmiştir. Bu çalışmada at yağ dokusu kaynaklı mezenkimal kök hücreler (EASCs) gelecekte hücresel tedavilerde kullanılabilirliği adına, morfolojileri ve çoklu farklılaşma kabiliyetleri bakımından değerlendirmeye alınmıştır. Elde edilen hücrelerin kök hücre özelliğini kesinleştirmek için hücreler osteojenik ve adipojenik farklılaştırmaya tabii tutulmuş ve nörosfer farklılaştırması yapılmıştır. Bunun yanı sıra, çalışmada elde edilen nörosferlerin enzimatik olarak çözülümünden sonra elde edilen nörosfer kaynaklı hücreler kültüre edilmiştir. Çalışma at yağ dokusu kaynaklı kök hücrelerin atlarda rejeneratif tıp için uygun bir seçim olabileceğini öne sürmektedir. Fakat at hekimliğinde pratik uygulama için bir standardın oluşturulmasından önce daha detaylı moleküler karakterizasyon çalışmalarına ihtiyaç duyulmaktadır.

Anahtar Kelimeler

At, Morfoloji, Çoklu farklılaştırma, Nörosfer, Kök hücre

Equine Adipose Tissue Derived Mesenchymal Stem Cells and Their Multilineage Differentiation

Öz

Mesenchymal stem cells (MSCs) have become a potent source for cell therapy due their inherent properties of self-renewal and ability to undergo unlimited divisions in the field of regenerative medicine. In this study, equine adipose tissue stem cells (EASCs) have been evaluated on the basis of their morphology, proliferation potential and multilineage differentiation capabilities for their future usage in cell therapy. The cells were further differentiated into osteogenic and adipogenic cell lineages as well as into adipose derived neurospheres to ensure the stemness of propagated cells. In our study, we further successfully cultured the neurosphere derived cells which were cultured from neurospheres after enzymatic dissociation. The study exhibits that EASCs offer a suitable choice for cellular regenerative therapy in equines. Nonetheless, there is a still need to molecular characterization of EASCs in order to establish a standard practice in equine medicine.

Anahtar Kelimeler

Equine, morphology, multilineage differentiation, neurosphere, Stem cells

Kaynakça

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