Protective Effect of Chlorogenic Acid Against Testicular Damage Induced By Glyphosate Isopropilamine in Rats

Yıl 2024, Cilt: 17 Sayı: 4, 334 - 340

Ruhi Türkmen , Yavuz Osman Birdane , Orkun Atik , Hasan Hüseyin Demirel , Türkan Türkmen Özay Güleş

https://doi.org/10.30607/kvj.1520615

Öz

The aim of this subchronic toxicity study was to determine the prophylactic effect of chlorogenic acid (CGA) on the histopathologic-histologic changes and oxidative stress induced by Glyphosate isopropylamine (GLF-ISO) in testicular tissues. A sum of 42 male Wistar rats were divided into six equal groups, each containing 7 rats. For pretreatment, rats were given CGA at doses of 12.5, 25 and 50 mg/kg (po) and GLF-ISO at 787.85 mg/kg (po) for 7 weeks. GLF-ISO significantly increased malondialdehyde levels while decreasing SOD and CAT activities and GSH levels in testicular tissues. On the contrary, these parameters were improved in CGA-treated groups. Furthermore, CGA ameliorated the histopathological and histological changes in testicular tissues induced by GLF-ISO in a dose-dependent manner. The results indicate that testicular damage caused by GLF-ISO can potentially be prevented or managed by CGA.

Anahtar Kelimeler

Glyphosate isopropilamine, Chlorogenic acid, Oxidative stress, Testicular damage

Etik Beyan

This study was carried out at Afyon Kocatepe University Reserch Animals Application Center. This research was approved by The Ethics Committee of the Faculty of Veterinary Medicine, Afyon Kocatepe University (AKUHADYEK, Ref No: 49533702/65, Tarih: 04/2018)

Destekleyen Kurum

Afyon Kocatepe University Scientific Research Projects Coordinators

Teşekkür

In this study would like to thank the Afyon Kocatepe University Scientific Research Projects Coordinators with the study entitled “Evaluation of the therapeutic efficacy of single and combined use of crocin, curcumin, and chlorogenic acid against intoxication induced by glyphosate isopropylamine in rats,” Project No: 18.KARİYER.244, for their contributions to the present study and also thank the administration and staff of the Afyon Kocatepe University Research Animals Application Center.

Sıçanlarda Glifosat İzopropilamin ile Oluşturulan Testiküler Hasara Karşı Klorojenik Asidin Koruyucu Etkisi

Öz

Bu subkronik toksisite çalışmasının amacı, testis dokularında Glifosat izopropilamin (GLF-ISO) tarafından indüklenen histopatolojik-histolojik değişiklikler ve oksidatif stres üzerinde klorojenik asidin (CGA) profilaktik etkisini belirlemektir. Toplam 42 erkek Wistar sıçan, her biri 7 sıçan içeren altı eşit gruba ayrılmıştır. Ön muamele için sıçanlara 7 hafta boyunca 12.5, 25 ve 50 mg/kg (po) dozlarında CGA ve 787.85 mg/kg (po) dozunda GLF-ISO verilmiştir. GLF-ISO testis dokularında malondialdehit seviyelerini önemli ölçüde artırırken SOD ve CAT aktivitelerini ve GSH seviyelerini azaltmıştır. Aksine, bu parametreler CGA ile tedavi edilen gruplarda iyileşmiştir. Ayrıca, CGA, GLF-ISO tarafından indüklenen testis dokularındaki histopatolojik ve histolojik değişiklikleri doza bağlı bir şekilde iyileştirmiştir. Sonuçlar, GLF-ISO'nun neden olduğu testis hasarının CGA ile potansiyel olarak önlenebileceğini veya yönetilebileceğini göstermektedir.

Anahtar Kelimeler

Glyphosate İzopropilamin, Klorojenik asit, Oksidatif stres, Testiküler hasar

Kaynakça

• Aebi, H. (1984). Catalase in vitro. In Methods in Enzymology (Vol. 105, pp. 121-126). Academic press Available from: http://www.ncbi.nlm.nih.gov/pubmed/6727660
• Ahbab, M.A., Barlas, N., & Karabulut, G. (2017). The toxicological effects of bisphenol A and octylphenol on the reproductive system of prepubertal male rats. Toxicol Ind Health., 33(2): 133–46.
• Avdatek, F., Birdane, Y.O., Türkmen, R., & Demirel, H.H. (2018). Ameliorative effect of resveratrol on testicular oxidative stress, spermatological parameters and DNA damage in glyphosate-based herbicide-exposed rats. Andrologia, 50(7): e13036.
• Avdatek, F., Kirikkulak, M., & Yeni, D. (2023). The effects of proanthocyanidin on testicular toxicity in rats exposed to a glyphosate-based herbicide. Pol J Vet Sci., 657–66.
• Beutler, E., Duron, O., & Kelly, B.M. (1963). Improved method for the determination of blood glutathione. J Lab Clin Med [Internet], 61:882–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/13967893
• Bhardwaj, J.K., Kumar, V., Saraf, P., Panchal, H., Rathee, V., & Sachdeva, S.N. (2022). Efficacy of N-acetyl- l-cysteine against glyphosate induced oxidative stress and apoptosis in testicular germ cells preventing infertility. J Biochem Mol Toxicol., 36(4): e22979.
• Clair, É., Mesnage, R., Travert, C., & Séralini, G.É. (2012). A glyphosate-based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro, and testosterone decrease at lower levels. Toxicology in Vitro. 26(2): 269–79.
• Culling, C.F.A., Allison, R.T., & Barr, W.T. (1984). Cellular pathology technique. Butterworths, London Dai, P., Hu, P., Tang, J., Li, Y., & Li, C. (2016). Effect of glyphosate on reproductive organs in male rat. Acta Histochem., 118(5): 519–26.
• Hanson, B. (2024). Glyphosate formulations - what’s the diff (and what’s a salt)? [Internet]. 2024 [cited 2024 Jul 17]. Available from: https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=25903
• Hashim, A.R., Bashir, D.W., Yasin, N.A.E., Rashad, M.M., & El-Gharbawy, S.M. (2022). Ameliorative effect of N-acetylcysteine on the testicular tissue of adult male albino rats after glyphosate-based herbicide exposure. J Biochem Mol Toxicol., 36(4): e22997.
• Hess, R.A. (1990). Quantitative and qualitative characteristics of the stages and transitions in the cycle of the rat seminiferous epithelium: light microscopic observations of perfusion-fixed and plastic-embedded testes. Biol Reprod., 43(3): 525–42.
• Jia, Z., Liu, S., Chen, T., Shi, Z., Li, X., Gao, Z., Zhang, Q., & Zhong, C. (2024). Chlorogenic acid can improve spermatogenic dysfunction in rats with varicocele by regulating mitochondrial homeostasis and inhibiting the activation of NLRP3 inflammasomes by oxidative mitochondrial DNA and cGAS/STING pathway. Bioorg Chem., 150: 107571.
• Juárez‐Rojas, L., Casillas, F., López, A., Betancourt, M., Ommati, M.M., & Retana‐Márquez, S. (2022). Physiological role of reactive oxygen species in testis and epididymal spermatozoa. Andrologia, 54(4).
• Leblond, C.P., & Clermont, Y. (1952). Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann N Y Acad Sci., 55(4): 548–73.
• Liu, J.B., Li, Z.F., Lu, L., Wang, Z.Y., & Wang, L. (2022). Glyphosate damages blood-testis barrier via NOX1-triggered oxidative stress in rats: Long-term exposure as a potential risk for male reproductive health. Environ Int., 159: 107038.
• Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem., 193(1): 265–75.
• Luna, L.G. (1968). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. New York,: Blakiston Division, McGraw-Hill.
• Nardi, J., Morasi, P.B., Koeppe, C., Dallegrave, E., Leal, M.B., & Rossato-Grando, L.G. (2017). Prepubertal subchronic exposure to soy milk and glyphosate leads to endocrine disruption. Food Chem Toxicol., 100: 247–52.
• National Pesticide International Center (2024). http://npic.orst.edu/factsheets/archive/glyphotech.html [Internet]. Glyphosate Technical Fact Sheet. [cited 2024 Jul 17]. Available from: http://npic.orst.edu/factsheets/archive/glyphotech.html
• Ohkawa, H., Ohishi, N., & Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem., 95(2): 351–8.
• Owagboriaye, F.O., Dedeke, G.A., Ademolu, K.O., Olujimi, O.O., Ashidi, J.S., & Adeyinka, A.A. (2017). Reproductive toxicity of Roundup herbicide exposure in male albino rat. Exp Toxicol Pathol., 69(7): 461–8.
• Pavuluri, H., Bakhtiary, Z., Panner Selvam, M.K., & Hellstrom, W.J.G. (2024). Oxidative Stress-Associated Male Infertility: Current Diagnostic and Therapeutic Approaches. Medicina (Baires), 60(6): 1008.
• Qi W, Zhao T, Yang W-W, Wang G-H, Yu H, Zhao H-X, Yang C, Sun L-X. (2011). Comparative pharmacokinetics of chlorogenic acid after oral administration in rats. J Pharm Anal., 1(4): 270–4.
• Sharma, R., Agarwal, A., Mohanty, G., Du Plessis, S.S., Gopalan, B., Willard, B., Yadav, S.P., & Sabanegh, E. (2013). Proteomic analysis of seminal fluid from men exhibiting oxidative stress. Reproductive Biology and Endocrinology, 11(1): 85.
• Spadella, M.A., Silva, E.J.R., Chies, A.B., & Almeida, L.A. (2024). Insights Into Antioxidant Strategies to Counteract Radiation-Induced Male Infertility. Antioxid Redox Signal, 40(13–15): 776–801.
• Sun, Y., Oberley, L.W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem., 34(3): 497–500.
• Tajik, N., Tajik, M., Mack, I., & Enck, P. (2017). The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature. Eur J Nutr., 56(7): 2215–44.
• Türk, G., Ceribaşı, A.O., Sahna, E., & Ateşşahin, A. (2011). Lycopene and ellagic acid prevent testicular apoptosis induced by cisplatin. Phytomedicine, 18(5): 356–61.
• Turkmen, R., & Dogan, I. (2020). Determination of acute oral toxicity of glyphosate isopropylamine salt in rats. Environ Sci Pollut Res Int., 27(16): 19298–303.
• Uz, E., Söğüt, S., Sahin, S., Var, A., Ozyurt, H., Güleç, M., & Akyol, O. (2002). The protective role of caffeic acid phenethyl ester (CAPE) on testicular tissue after testicular torsion and detorsion. World J Urol., 20(4): 264–70. Wikipedia (2024). Glyphosate [Internet]. 2024 [cited 2024 Jul 17]. Available from: https://en.wikipedia.org/wiki/Glyphosate