Immunomodulatory Effects of Inactive Parapoxvirus Ovis Administration to Pregnant Heifers on Colostrum and Calf Health

Yıl 2025, Cilt: 18 Sayı: 1, 43 - 51

Halime Kara , Mehmet Şahal

Öz

The aim of this study was to evaluate the effect of inactive Parapoxvirus ovis (IPPVO) immunomodulation protocol administration to pregnant heifers in the third trimester of pregnancy on colostrum quality, passive transfer success and calf health. Animal material of the study included 40 Holstein breed pregnant heifers and their calves, 20 of them was IPPVO group and rest 20 was control group. Totally 3 doses of 2 ml IPPVO (Zylexis, Zoetis, USA) was administered intramuscularly to study group in 25, 23 and 21 days before estimated birth date. Also control group was injected the same amount of isotonic NaCI %0.9 with IPPVO in the same days. Colostrum samples from heifers after birth and blood samples from calves 24-48 hours after birth were taken and sera were extracted. Immunoglobulin G (Ig G) concentrations of colostrum and serum samples were measured by using enzyme linked immunoassay (ELISA) method. Calves’ health status were observed during first 30 days. There was no adverse effect was detected in heifers administered IPPVO during study procedure. IgG concentrations of heifers that received IPPVO was 59.64 mg/ml; that of control group was 59.62 mg/ml and the difference between groups was statistically insignificant (p>0.05). Average serum IgG concentration of calves born from heifers that received IPPVO was 32.6 mg/ml; that of control group was 28.7 mg/ml. When the yielded results of daily health status controls of two claves groups evaluated, no statistically significant difference was detected in terms of developing any disease and calf deaths (p>0.05).

Anahtar Kelimeler

Colostrogenesis, Holstein Pregnant Heifer, IgG, Immunomodulation, Inactive Parapoxvirus Ovis

Kocatepe Gebe Düvelerde İnaktif Parapoxvirus Ovis Uygulamasinin Kolostrum ve Buzaği Sağliği Üzerine İmmunmodulatorik Etkileri

Öz

Sunulan bu çalışmanın amacı sığırlarda gebeliğin son döneminde uygulanan inaktif Parapoxvirus Ovis (İPPVO) immunmodulasyon protokolünün kolostrum kalitesi, pasif transfer başarısı ve buzağı sağlığı üzerine etkilerinin araştırılmasıdır. Çalışmanın hayvan materyalini 20 kontrol ve 20 İPPVO grubu olmak üzere 40 adet Holstein ırkı gebe düve ve buzağıları oluşturdu. İPPVO grubuna muhtemel doğum tarihlerine 25, 23 ve 21 gün kala, toplamda 3 doz, 2 ml lik İPPVO preparatı, intramusküler (Zylexis, Zoetis, USA), kontrol grubuna ise aynı planda ve hacimde izotonik NaCI %0.9 enjeksiyonu yapılmıştır. Doğumla birlikte annelerden kolostrum örnekleri, buzağılardan ise doğumdan 24 – 48 saat sonra kan alınarak serumları çıkartılmıştır. Kolostrum ve serum örneklerinde ELISA yöntemi kullanılarak IgG konsantrasyonları ölçülmüştür. Buzağılar ilk 30 günlük dönemde sağlık yönünden takip edilmiştir. Çalışma kapsamında İPPVO preperatı kullanılan düvelerde herhangi bir yan etki gözlenmemiştir. İPPVO uygulanan düvelerin kolostrum IgG konsantrasyonları 59.64 mg/ml, kontrol grubunda ise 59.62 mg/ml olarak ölçülmüş ve aradaki fark istatistik yönden anlamsız bulunmuştur (p>0.05). İPPVO uygulanan düvelerden doğan buzağıların ortalama kan serumu IgG konsantrasyonu 32.6 mg/ml, kontrol grubunda 28.7 mg/ml olarak ölçülmüştür. Çalışma kapsamında iki grup buzağıları arasında yapılan günlük sağlık kontrolleri sonucunda toplanılan veriler değerlendirildiğinde hastalığa yakalanma ve buzağı ölümleri yönünden de anlamlı bir farklılık tespit edilmemiştir (p>0.05).

Anahtar Kelimeler

Gebe Holstein Düve, IgG, İmmunmodülasyon, İnaktif Parapoxvirus Ovis, Kolostrogenezis

Kaynakça

• Adams, A.A. & Horohov, D.W. (2013). The Effect of an Immunomodulator (parapoxvirus ovis) on Cell-Mediated Immunity (CMI) in Abruptly Weaned Foals. Veterinary Immunology and Immunopathology, 153(1), 118-122. https://doi.org/10.1016/j.vetimm.2012.11.020
• Akyüz, E., Naseri, A., Erkiliç, E.E., Makav, M., Uzlu, E., Kirmizigül, A.H. & Gökçe, G. (2017). Neonetal Buzağı İshalleri ve Sepsis. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2), 181-191.
• Atlı, K., & Şimşek, A. (2022). Effect of attenueted parapoxvirus ovis on levels of some serum cytokines and antibody response in sheep. Eurasian Journal of Veterinary Sciences, 38(2), 122-130. https://doi.org/10.15312/EurasianJVetSci.2022.373
• Aydin, Ö. & Aktaş, M.S. (2021). Hayvanlarda Kullanılan Bazı İmmunstimulanların Genel Özellikleri ve Farklı Hayvan Türlerinde Yapılan Çalışmalar. Bozok Veterinary Sciences, 2(2), 108-114.
• Aydoğdu, U. & Güzelbekteş, H. (2018). Effect of colostrum composition on passive calf immunity in primiparous and multiparous dairy cows. Veterinarni Medicina, 63(1), 1-11. https://doi.org/10.17221/40/2017-VETMED
• Aydoğdu, U., Şen, İ. & Güzelbekteş, H. (2019). Buzağılarda Pasif Transfer Yetmezliğinin Belirlenmesinde Kullanılan Yöntemler. Manas Journal of Agriculture Veterinary and Life Sciences, 9(2), 104-111. Besser, T.E. & Gay, C.C. (1994). The Importance of Colostrum to the Health of the Neonatal Calf. Veterinary Clinics of North America: Food Animal Practice, 10(1), 107-117. https://doi.org/ 10.1016/S0749-0720(15)30591-0
• Cao, Y., Zhao, D., Xu, A-T., Shen, J. & Ran, Z-H. (2015). Effects of Immunosuppressants on Immune Response to Vaccine in Inflammatory Bowel Disease. Chinese Medical Journal, 128(6), 835-838. https://doi.org/10.4103/0366-6999.152683
• Cervenak, J. & Kacskovics, I. (2009). The neonatal Fc receptor plays a crucial role in the metabolism of IgG in livestock animals. Veterinary Immunology Immunopathology, 128, 171–177. https://doi.org/10.1016/j.vetimm.2008.10.300
• Conneely, M., Berry, D.P., Sayers, R., Murphy, J.P., Lorez, I., Doherty, M.L. & Kennedy, E. (2013). Factors associated with the concentration of immunoglobulin G in the colostrum of dairy cows. Animal, 7, 1824–1832. https://doi.org/10.1017/S1751731113001444
• Cortese, V.S. (2009). Neonatal Immunology. Veterinary Clinics of North America: Food Animal Practice, 25(1), 221-227. https://doi.org/10.1016/j.cvfa.2008.10.003
• Çalık, H. (2016). Buzağı İshallerine Karşı Aşılanan Gebe Düvelerde Corynebacterium cutis Lizatının Kolostrum İmmunglobulin G Düzeylerine Etkisi. Yüksek Lisans, Erciyes Üniversitesi, Kayseri, Türkiye.
• Dhama, K., Saminathan, M., Jacob, S.S., Singh, M., Karthik, K., Amarpal., Tiwari, R., Sunkara, L.T., Malik, Y.S. & Singh, R.K. (2015). Effect of immunomodulation and immunomodulatory agents on health with some bioactive principles, modes of action and potent biomedical applications. International Journal of Pharmacology, 11, 253-290.
• Erbasan, S., Mamak, N. (2023). Investigation of the immunomodulatory effect of inactive parapoxvirus (İPPVO) on infectious bovine rhinotracheitis (IBR) vaccine in cattle. MAE Vet Fak Derg, 8(3), 183-189. https://doi.org/10.24880/maeuvfd.1310119
• Fachinger, V., Schlapp, T. & Saalmuller, A. (2000). Evidence for a parapox ovis virus- associated superantigen. European Journal of Immunology, 30, 2962–2971. https://doi.org/10.1002/1521-4141(200010)30:10<2962::AID-IMMU2962>3.0.CO;2-1
• Fleming, S.B. & Mercer, A.A. (2007). Genus Parapoxvirus. In: Mercer AA, Schmidt A, Weber O (eds) Poxviruses. Basel, Switzerland: Birkhäuser Verlag.
• Galeotti, M. (1998). Some aspects of the application of immunostimulants and a critical review of methods for their evaluation. Journal of Applied Ichthyology, 14, 189-199. https://doi.org/10.1111/j.1439-0426.1998.tb00641.x
• Godden S (2008). Colostrum management for dairy calves. Veterinary Clinics of North America: Food Animal Practice, 24(1), 19-39. https://doi.org/10.1016/j.cvfa.2007.10.005
• Gökçe, E. & Erdoğan, H.M. (2013). Neonatal buzağılarda kolostral immunglobulinlerin pasif transferi. Türkiye Klinikleri Veteriner Bilimleri, 4(1), 18-46
• Gulliksen, S.M., Lie, K.I., Solverod, L. & Osteras, O. (2008). Risk factors associated with colostrum quality in Norwegian dairy cows. Journal of Dairy Science, 91(2), 704-712. https://doi.org/10.3168/jds.2007-0450
• Güngör, Ö. & Baştan, A. (2004). Effects of vaccination on colostrum and calf blood serum IgG concentrations in pregnant cows. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 51(1), 13-1
• Güzelbekteş, H. V Aydoğdu, U. (2024). Buzağılarda pazif transfer yetmezliği. Sevinç M, editör. Buzağı Sağlığı ve Yönetimi. 1. Baskı. Ankara: Türkiye Klinikleri.
• Hall, J.A., Bobe, G., Vorachek, W.R., Estill, C.T., Moster, W.D., Pirelli, G.J. & Gamroth, M. (2014). Effect of supranutritional maternal and colostral selenium supplementation on passive absorption of immunoglobulin G in selenium-replete dairy calves. Journal of Dairy Science, 97, 4379-4391. https://doi.org/10.3168/jds.2013-7481
• Heinrichs, A.J., Jones, C.M. & Heinrichs, B.S. (2003). Effects of mannan oligosaccharide or antibiotics in neonatal diets on health and growh of dairy calves. Journal of Dairy Science, 86(12), 4064-4069. https://doi.org/10.3168/jds.S0022-0302(03)74018-1
• Hurley, W.L. & Theil, P.K. (2011). Perspectives on immunoglobulins in colostrum and milk. Nutrients, 3(4), 442-474. https://doi.org/10.3390/nu3040442
• Husband, A.J., Brandon, M.R. & Lascelles, A.K. (1972). Absorption and endogenous production of immunoglobulins in calves. Australian Journal of Experimental Biology and Medical Science, 50, 491–498. https://doi.org/10.1038/icb.1972.41
• Jaster, E.H. (2005). Evaluation of quality, quantity and timing of colostrum feeding on immunoglobulin G1 absorption in Jersey calves. Journal of Dairy Science, 88(1), 296-302. https://doi.org/ 10.3168/jds.S0022-0302(05)72687-4
• Johnson, K.F., Chancellor, N., Burn, C.C. & Wathes, D.C. (2017). Prospective cohort study to assess rates of contagious disease in pre-weaned UK dairy heifers: management practices, passive transfer of immunity and associated calf health. Veterinary Record, 4, e000226. https://doi.org/10.1136/vetreco-2017-000226
• Kara, E., Terzi, O.S., Şenel, Y. & Ceylan, E. (2020). Yerli Kara ve İsviçre Esmeri Irkı Sığırların Kolostrum Kalitesinin Karşılaştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 34(3), 153-156.
• Kara, E. & Ceylan, E. (2021). Failure of passive transfer in neonatal calves in dairy farms in Ankara region. Turkish Journal of Veterinary & Animal Sciences, 45, 556-565. https://doi.org/ 10.3906/vet-2011-26
• Kart, A., Uzlu, E., Karapehlivan, M., Öğün, M. & Merhan, O. (2010). Buzağılarda İmmunmodülatör Zylexis’in Kan Glutatyon, Malondialdehit, Nikrik Oksit ve Toplam ve Lipit Bağlı Sialik Asit Seviyelerine Etkileri. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 5(2), 43-48.
• Kaygısız, A. & Köse, M. (2007). The quality of colostrum and its effects on calves growth characteristics in holstein cattle. Journal of Agricultural Sciences, 13(4), 321-325. https://doi.org/ 10.1501/Tarimbil_0000000403
• Kehoe, S.I., Heinrichs, A.J., Moody, M.L., Jones, C.M. & Long, M.R. (2011). Comparison of immunoglobulin G concentrations in primiparous and multiparous bovine colostrum. The Professional Animal Scientist, 27(3), 176-180. https://doi.org/10.15232/S1080-7446(15)30471-X
• Kim, S.W., Mateo, R.D., Yin, Y.L. & Wu, G. (2007). Functional amino acids and fatty acids for enhancing production performance of sows and piglets. Asian-Australasian Journal of Animal Sciences, 20(2), 295-306. https://doi.org/10.5713/ajas.2007.295
• Koçak, S., Yüceer, B., Uğurlu, M. & Özbeyaz, C. (2007). Bala Tarım İşletmesinde Yetiştirilen Holştayn İneklerde Bazı Verim Özellikleri. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 47(1), 9-14.
• Koçak, S., Tekerli, M., Özbeyaz, C. & Demirhan, İ. (2008). Lalahan Merkez Hayvancılık Araştırma Enstitüsün’de Yetiştirilen Holştayn, Esmer ve Simental Sığırlarda Bazı Verim Özellikleri. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 48(2), 51-57.
• Krakowski, L., Krzyzanowski, J., Wrona, Z. & Siwickia, K. (1999). The effect of nonspecific immunostimulation of pregnant mares with 1.3/1.6 glucan and levamisole on the immunoglobulins levels in colostrum, selected indices of nonspecific cellular and humoral immunity in foals in neonatal and postnatal period. Veterinary Immunology and Immunopathology, 68(1), 1-11. https://doi.org/10.1016/S0165-2427(99)00006-9
• Kurtdede, E., Kurtdede, A., Kara, E., Başer, M. & Gülendağ, E. (2022). Relationships between the Postpartum Body Condition Score, Colostral IgG Content, Liver Lipid Metabolism and Oxidative Stress in Dairy Cows. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 36(1), 16-21.
• Lombard, J., Urie, N., Garry, F., Godden, S., Quigley, J., Earleywine, T., McGuirk, S., Moore, D., Branan, M., Chamorro, M., Smith, G., Shivley, C., Catherman, D., Haines, D., Heinrichs, A.J., James, R., Maas, J. & Sterner, K. (2020). Consensus recommendations on calf-and herd-level passive immunity in dairy calves in the United States. Journal of Dairy Science, 103, 7611-7624. https://doi.org/10.3168/jds.2019-17955
• Mohamed, E.M., Ibrahim, E.S. & Mohamed, F.S. (2013). Effect of administration of ultra-corn with bivalent Foot and Mouth disease oil vaccine in calves. Veterinary World, 6(8), 486-492. https://doi.org/10.5455/vetworld.2013.486-492
• Nandi, S., De, U.K. & Chowdhury, S. (2007). Current status of contagious ecthyma or orf disease in goat and sheep-A global perspective. Small Ruminant Research, 96, 73–82. https://doi.org/10.1016/j.smallrumres.2010.11.018
• Orta, Y. S., Atlı, K., & Kale, M. (2020). Vaccines used in Echtyma disease and alternative treatment methods. Current Perspectives on Health Sciences, 3(2), 82-91.
• Phipps, A.J., Beggs, D.S., Murray, A.J., Mansell, P.D. & Pyman, M.F. (2017). Factors associated with colostrum immunoglobulin G concentration in northern‐ Victorian dairy cows. AUSTRALIAN VETERINARY JOURNAL, 95(7), 237-243. https://doi.org/10.1111/avj.12601
• Pirofski, L.A. & Casadevall, A. (2006). Immunomodulators as an antimicrobial tool. Current Opinion in Microbiology, 9, 489-495. https://doi.org/10.1016/j.mib.2006.08.004
• Quigley, J. (2007). Passive Immunity in Newborn Calves. Advances in Dairy Technology, 19, 247-265. Sancak, S. & Gülhan, T. (2021). Gebe Sığırlarda İnaktif Escherichia coli Aşısının Kolostrum ve Buzağı Kan Serumlarındaki IgG Seviyelerine Etkileri. Journal of Anatolian Environmental and Animal Sciences, 6(3), 318-324. https://doi.org/10.35229/jaes.886678
• Saucedo-Quintero, J.S., Avendaño-Reyes, L., Alvarez-Valenzuela, F.D., Rentería-Evangelista, T.B., Moreno-Rosales, J.B., Montaño-Gómez, M.F., Medina-Basulto, G.R., Gallegos-De, L.A. & Hoya, M.P. (2004). Colostrum immunoglobulins transference in Holstein cattle according the age of the dam. American Society of Animal Science, 55, 322-324.
• Swan, H., Godden, S., Bey, R., Wells, S., Fetrow, J. & Chester-Jones, H. (2007). Passive transfer of immunoglobulin G and preweaning health in Holstein calves fed a commercial colostrum replacer. Journal of Dairy Science, 90(8), 3857-3866. https://doi.org/10.3168/jds.2007-0152
• Şahal, M., Terzi, O.S., Ceylan, E. & Kara, E. (2018). Buzağı İshalleri ve Korunma Yöntemleri. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 58(Özel Sayı), 41-49.
• Şahin, A. & Ulutaş, Z. (2010). Polatlı Tarım İşletmesinde Yetiştirilen Siyah Alaca İneklerde Süt ve Döl Verim Özellikleri. Anadolu Tarım Bilimleri Dergisi, 25(3), 202-212.
• Şentürk, S., Polat, Ü. & Kennerman, E. (2003). Kuru Dönemde Levamizol Uygulanan İneklerden Doğan Buzağıların İmmun Düzeylerinin Belirlenmesi. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 22(1-2-3), 7-11.
• Şimşek, G. & Akkan, H.A. (2021). The relationships between passive transfer failure and neonatal diseases in calves in Burdur province. Current Perspectives on Health Sciences, 2(1), 37-43. Thacker, E.L. (2010). Immunomodulators, Immunostimulants, and Immunotherapies in Small Animal Veterinary Medicine. Veterinary Clinics of North America: Small Animal Practice, 40(3), 473-483. https://doi.org/10.1016/j.cvsm.2010.01.004
• Turna Yilmaz, Ö., Kaşikçi, G. & Gündüz, M.C. (2011). Benefits of pregnant sheep immunostimulantion with Corynebacterium cutis on post-partum and early newborn’s life IgG levels, stillbirth rete and lamb’s weighht. Small Ruminant Research, 97(11-3), 146-151. https://doi.org/10.1016/j.smallrumres.2011.03.002
• Urie, N.J., Lombard, J.E., Shivley, C.B., Kopral, C.A., Adams, A.E., Earleywine, T.J., Olson, J.D. & Garry, F.B. (2018). Preweaned heifer management on US dairy operations: Part V. Factors associated with morbidity and mortality in preweaned dairy heifer calves. Journal of Dairy Science, 101, 9229-9244. https://doi.org/10.3168/jds.2017-14019
• Waldner, C. & Rosengren, L.B. (2009). Factors associated with serum immunglobulin levels in beef calves from Alberta and Saskatchewan and association between passive transfer and health outcomes. The Canadian Veterinary Journal, 50(3), 275-281.
• Wang, R. & Luo, S. (2018). Orf virus: a new class of immunotherapy drugs. Vlachakis D. eds. In: Systems Biology. Athens, Greece: IntechOpen.
• Yanar, K.E. & Aktaş, M.S. (2021). Bitkisel Orijinli İmmunmodulatörler ve Hayvan Sağlığında Kullanım Olanakları. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi, 9(1), 849-855. https://doi.org/10.18586/msufbd.879022 Yüceer, B. & Özbeyaz, C. (2010). Kolostrum Almış Buzağılarda Bağışıklığın, Büyüme, Hastalık insidansı ve yaşama gücü üzerine etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 57(3), 185-190. https://doi.org/10.1501/Vetfak_0000002375
• Zarcula, S., Cernescu, H. & Knop, R. (2008). Colostral İmmunıty in Newborn Calf: Methods for Improvement of Immunoglobulins Absorption. LUCRĂRI ŞTIINłIFICE MEDICINĂ VETERINARĂ TIMISOARA, XLI, 195-202.