In vitro Embriyo Kültür Medyumlarına İlave Edilen Eritropoietinin Oksidatif Stres ve Embriyo Gelişimi Üzerine Etkisi

Yıl 2019, Cilt: 59 Sayı: Ek Sayı, 107 - 112, 04.06.2020

Muharrem Satılmış , Ali Bilgili

Öz

Çalışmada, in vitro embriyo kültür medyumuna ilave edilen eritropoietinin antioksidan etkinliği incelendi. Sığır ovaryumlarından elde edilen oositlerin in vitro maturasyonu ve fertilizasyonlarını izleyen süreçte eritropoietinin 3 farklı dozu (0,5 μg, 0,25 μg, 0,125 μg /ml) ilave edilerek 3 deneme grubu ve eritropoietin ilave edilmemiş (antioksidansız) kontrol grubu oluşturularak kültür ortamına alınarak bölünen oosit oranları, blastosiste erişim oranları biyokimyasal parametreler değerlendirildi. Morula ve 48. saat bölünen oosit oranları bakımından gruplar arasındaki farklılık önemli iken, diğer özellikler için gruplar arası farklılıkların istatistiksel olarak önemsiz olduğu tespit edildi (p>0,05).

Anahtar Kelimeler

Antioksidan, embriyo, eritropoietin, in vitro, oksidatif stres

Kaynakça

• 1. Brackett BG, Zuelka KA (1993): Analysis of factor involved in the in vitro production of bovine embryos. Therio, 39: 43-64.
• 2. Bucak MN, Satılmış M, Kızıl SH, Karaşahin T, Akyol N (2010): Sığır embriyolarının in vitro gelişiminde kültür medyumlarına katılan antioksidanların etkisi. Kafkas Üniv Vet Fak Derg, 16 (1): 69-74.
• 3. Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S (2003): Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients. Int J Clin Pharmacol Ther, 41: 187-192.
• 4. Del Corso A, Cappiello M, Mura U (1994): Thiol-dependent oxidation of enzymes: the last chance against oxidative stress. Int J Biochem, 26: 745-750.
• 5. Düzgüneş O, Kesici T, Gürbüz F (1983): İstatistik Metotları I. Ankara Üniversitesi Ziraat Fakültesi Yayınları, 861: 229.
• 6. Gasparrini B, Neglia G, Di Palo R, Campanile G, Zircarelli L (2000): Effect of cysteamine during in vitro maturation on buffalo embryo development. Therio, 54: 1537-1542.
• 7. Halliwell B, Gutteridge JMC (2000): Free radicals in biology and medicine. 3th ed. Oxford: Oxford Science Publications, 617- 24.
• 8. Hamano S, Koikeda A, Kuwayama M, Nagai T (1992): Full-term development of in vitro matured, vitrified and fertilized bovine oocytes. Therio, 38: 1085-1090.
• 9. İnal M, Kanbak G, Şen S, Akyüz F, Sunal E (1999): Antioxidant status and lipid peroxidation in hemodialysis patients undergoing erythropoietin and erythropoietin- vitamin E combined therapy. Free Radic Res, 31: 211-216.
• 10. Izquierdo D, Villamediana P, Paramio MT (1999): Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes. Therio, 52: 847-61.
• 11. Johnson MH, Nasr-Esfahani MH (1994): Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro. Bioessays, 16: 31-38.
• 12. Kanagawa H, Shimohira I, Saitoh N (1995): Manuel of bovine embryo transfer national livestock breeding center. MAFF, JICA, Japan.
• 13. Kehrer JP, Lund LG (1994): Cellular reducing equivalents and oxidative stress. Free Radical Biol Med, 17: 65-75.
• 14. Kratz SB (1991): Erythropoietin. Blood, 77: 419-434
• 15. Maiese K, Li F, Chong ZZ (2005): New avenues of exploration for erythropoietin. JAMA, 293: 90-95.
• 16. Maxwell PH, Ferguson DJ, Nicholls LG, Iredale JP, Pugh CW, Johnson MH, Ratcliffe PJ (1997): Sites of erythropoietin production. Kidney Int, 51: 393-401.
• 17. Mimic-Oka J, SIMIC T, DJUKANOVIC L (2002): Epoetin treatment improves red blood cell and plasma antioxidant capacity in hemodialysis patients. Ren Fail, 24: 77-87.
• 18. Nasr-Esfahani MH, Johnson MH (1992): How does transferrin overcome the in vitro block to development of the mouse preimplantation embryo? J Reprod Fertil, 96(1): 41-48
• 19. Sasaki, R, Masuda S, Nagao M (2000): Erythropoietin: multiple physiological functions and regulation of biosynthesis. Biosci Biotechnol Biochem, 64: 1775-1793.
• 20. Schellender K, Fuhrer F, Brackett BG, Korb H, Schleger W (1990): In vitro fertilization and cleavage of bovine oocytes matured in medium supplement with estrous cows serum. Therio, 33 (2): 477-485.
• 21. Sung LY, Du F, Xu J, Chang W, Nedambale TL, Zhang J, Jiang S, Tian XC, Yang X (2004):The differential requirement of albumin and sodium citrate on the development of in vitro produced bovine embryos. Reprod Nut Dev, 44, 551-564.
• 22. Takagi Y, Mori K, Takahashi T, Sugawara S, Masaki J (1992): Differences in development of bovine oocytes recovered by aspiration or by mincing. J Anim Sci, 70: 1923-1927.
• 23. Umaoka Y, Noda Y, Narimoto K, Mori T (1992): Effects of oxygen toxicity on early development of mouse embryos. Mol Reprod Dev, 31: 28-33.
• 24. Usberti M, Gerardi, GM, Micheli AM, Tira P, Bufano G, Gaggia P, Movilli E, Galli F (2002): Effects of erythropoietin and vitamin E modified me.brane on plasma oxidative stress markers and anemia of hemodialyzed patients. Am J Kidney Dis, 40: 590-599.
• 25. Wan PC, Hao Z.D, Zhou P, Wu Y, Yang L, Cui MS, Liu SR, Zeng SM (2008): Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Animal Reproduction Science, in pres.
• 26. Wang L, Zhang Z, Wang Y, Zhang R, Chopp M (2004): Treatment of stroke with erythropoietin enhances neurogenesis and angiogenesis and improves neurological function in rats. Stroke, 35: 1732-1737.