Sucul Organizmalarda Demir Klorür/ Zeolit Uygulamasının Oksidatif Stres Üzerine Etkisinin Belirlenmesi İçin Gökkuşağı Alabalığında (Oncorhynchus mykiss) Detoksifikan Modellemesi

Öz

Çeşitli aktiviteler sonucunda yer altı ve yer üstü sularının kirlenmesi ile yerli balık popülasyonları ve sucul ekosistemler olumsuz etkilenmektedir. Su kolonu ve sedimentte meydana gelen kirlilikte organik sentez reaksiyonlarının indirgenme maddesi olarak kullanılan demir klorür (FeCl₂) sonucunda demir tuzları bazı akuatik canlılar için toksik olabilmektedir. Bu etkileri minimize etmek adına özellikle son yıllarda zeolit gibi doğal ürünlerin kullanımı yaygınlaşmaktadır. Bu amaçla çalışmamızda, gökkuşağı alabalıkları (Oncorhynchus mykiss) 28 gün boyunca FeCl₂ ve/veya zeolite maruz bırakılarak oksidadif stres cevapları araştırılmıştır. Deneme süresi sonunda karaciğer ve böbreklerden alınan örneklerde oksidatif stres cevapları antioksidan enzim aktiviteleri ile belirlenmiştir. Kontrol ve muamele gruplarında böbrek ve karaciğer dokuları için CAT, SOD, GPx ve MDA değerleri istatistiki olarak önemli bulunmuştur (p<0,05). Bu çalışmada, enzim aktiviteleri açısından zeolit içeren gruplar daha düşük değerler vermiş ve zeolitin akuatik canlılar için koruyucu etkisi biyokimyasal parametrelerle desteklenmiştir.

Anahtar Kelimeler

• Zeolit,balık; oksidatif stres; enzim; detoksifikant

Is Zeolite a Detoxificant: Modelling of Ferrous Chloride/Zeolite Application of Aquatic Organisms on Rainbow Trout (Oncorhynchus mykiss) to Determine Its Effects on Oxidative Stress

Abstract

Populations of native fish and aquatic ecosystems have been negatively affected by the contamination of ground and surface waters as a result of various activities. Due to the ferrous chloride (FeCl₂), which is used as the reducing agent for the organic synthesis reactions in the contamination of water column and sediment, iron salts may be very toxic for some aquatic organism. In order to minimize these effects, natural products such as zeolite have been widely used recently. For this reason, rainbow trout (Oncorhynchus mykiss) were exposed to FeCl₂ and/or zeolite for 28 days and their oxidative stress responses were investigated. At the end of the treatment period, oxidative stress responses were determined with antioxidant enzyme activities in the samples taken from liver and kidneys. CAT, SOD, GPx and MDA values for kidney and liver tissues were found statistically important between control and treatment groups (p<0.05). In this study, zeolite application provided lower values in terms of enzyme activities, and the protective effect of zeolite for aquatic organism was supported by biochemical parameters. 

Keywords

• Zeolite,fish; oxidative stress; enzyme; detoxificant

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