Shiitake Mantar Ekstraktı İlaveli Yemlerin Gökkuşağı Alabalığında (Oncorhynchus mykiss) Büyüme, Non-Spesifik İmmun Parametreler ve in-vitro Aeromonas hyrophila Enfeksiyonuna Karşı Direnç Üzerine Etkileri

Öz

Bu çalışmada Lentinula edodes (shiitake) tıbbi mantar ekstraktının gökkuşağı alabalığının (Oncorhynchus mykiss) spesifik olmayan bağışıklık cevabı ve biyometrik performans üzerine etkileri incelenmiştir. Bu amaçla balıklar (başlangıç ağırlığı 20 g) iki tekrar olucak şekilde %1 ve %2 shiitake ekstraktı ilaveli yemlerle beslenen deneme grupları ve bir kontrol grubu olmak üzere üç gruba (60 balık/grup) ayrılmıştır. Deneme 45 gün sürmüştür. Beslenme sürecinde balıklardan 1., 2., 3., 4., 5. ve 6. haftalarda alınan kan ve serum örneklerinden immünolojik, biyokimyasal parametreler belirlenmiştir. Deneme başlangıcında ve sonunda balıklardan gerekli ölçümler yapılarak biyometrik analizler değerlendirilmiştir. İmmünolojik, biyokimyasal ve biyometrik parametrelerin sonuçlarına göre maksimum etkinin %2 shiitake ekstraktı ile beslenen gökkuşağı alabalığında meydana geldiğini belirlenmiştir. Deneme gruplarında balıkların kanında tespit edilen respiratory burst aktivitesi, kontrol grubuyla karşılaştırıldığında 2. ve 6. haftalarda artış gösterdiği tespit edilmiştir. Mantar ekstraktı ilaveli yemlerle beslenen balıklarda toplam protein miktarı, bakterisidal aktivitede önemli ölçüde artış göstermiştir. 3. ve 6. haftalarda %2 shiitake ekstraktı ilaveli yemlerle beslenen balıklarda kolesterol seviyesinin düştüğü belirlenmiştir. Elde edilen sonuçlara göre shiitake tıbbi mantar ekstraktı ilaveli yemlerle beslenen gökkuşağı alabalığının spesifik olmayan bağışıklık cevabının kontrol grubuna kıyasla ölçülen parametrelerde pozitif sonuçlar verdiği, aynı zamanda gökkuşağı alabalığının genel büyüme performansını arttığı belirlenmiştir.

Anahtar Kelimeler

• gökkuşağı alabalığı
• shiitake
• ekstrakt
• stimulate
• non-spesifik sistem

Proje Numarası

12/76

Effects of Dietary Supplemented Shiitake Mushroom Extract on Growth, Non-specific Immune Parameters and in-vitro Resistance Against Aeromonas hydrophila in Rainbow Trout (Oncorhynchus mykiss)

Öz

The activity of Lentinula edodes (shiitake) medicinal mushroom extract were examined on the non-specific immune response and biometrical performance of rainbow trout (Oncorhynchus mykiss). Fish (20 g initial weight) were divided into 3 treatment groups (60 fish/group) and duplicated groups for 6 weeks having two experimental diets supplemented with 1-2% shiitake extract and a control diet. During the feeding process, immunological, biochemical, and biometrical observations were determined using the fish and blood samples taken at weeks 1, 2, 3, 4, 5, and 6, respectively. The results of immunological, biochemical, and biometrical parameters evaluation determined that the maximum influence occurs in rainbow trout fed with 2% shiitake extract. The amount of respiratory burst activity in the blood of fish in the trial groups significantly increased in each diet on the 2nd and 6th weeks compared to controls. The amount of total protein, bactericidal activity were significantly increased in fish being fed a mushroom supplemented diet. Cholesterol level decreased in fish blood, which fed with 2% shiitake extract supplemented diet at 3rd and 6th weeks. These results support the findings that the non-specific immune responses of rainbow trout was stimulated in fish by feeding shiitake medicinal mushroom extract yielding positive results in measured parameters compared to the control group also enhancing the overall growth performance of rainbow trout.

Anahtar Kelimeler

• rainbow trout
• shiitake
• extract
• stimulate,
• innate immunity

Destekleyen Kurum

Muğla Sıtkı Koçman University Scientific Research Project Coordination Unit

Proje Numarası

12/76

Teşekkür

We thank to Süleyman BABA for him support during trial. We also thank to Dr. Vikas Kumar for valuable comments on manuscript. This study is a part of the PhD. dissertation of Esin Baba.

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