Evaluation of Antibacterial Activity of Commercial Lemon, Geranium, and Thistle Oils Against Pseudomonas Species

Öz

Increasing antibiotic resistance is increasingly affecting human and animal health. Pseudomonas spp. are opportunistic pathogens resistant to multiple antibiotics. Pseudomonas spp. can be found in various environmental sources, including surface water, wastewater, food, and soil, and can cause hospital-associated infections. Therefore, due to the increasing resistance to antibiotics, plants and their extracts are being investigated as alternative natural sources of antibiotics. Thus, in this study, the antibacterial activities of commercially available thistle (Silybum marianum) fixed oil, lemon (Citrus limon), and geranium (Pelargonium graveolens) essential oils against Pseudomonas aeruginosa, Pseudomonas putida, and Pseudomonas monteilii isolated from used makeup sponges, as well as Pseudomonas aeruginosa ATCC 27853 and ATCC 10145, were evaluated in vitro using agar well diffusion and microdilution methods. It was determined that thistle fixed oil, lemon, and geranium essential oils did not give an inhibition zone against the tested Pseudomonas species, and their MIC values were greater than 80 μg/mL.

Anahtar Kelimeler

• Citrus limon
• Pelargonium graveolens
• Silybum marianum
• Pseudomonas species
• Antibacterial activity

Ticari Limon, Sardunya (Itır) ve Deve Dikeni Yağlarının Pseudomonas Türlerine Karşı Antibakteriyel Aktivitesinin Değerlendirilmesi

Öz

Artan antibiyotik direnci insan ve hayvan sağlığını her geçen gün etkilemektedir. Pseudomonas türleri ise birden çok antibiyotiğe karşı dirençli fırsatçı bir patojendir. Pseudomonas türleri yüzey suyu, atık su, gıda ve toprak gibi çevresel kaynaklardan da bulunabilmekte ve hastane ilişkili enfeksiyonlara neden olmaktadır. Bu nedenle artan antibiyotik direnci sebebiyle de antibiyotiklere alternatif doğal kaynaklar olan bitkiler ve onların ekstraktları araştırılmaktadır. Bu nedenle bu çalışmada kullanılmış makyaj süngerlerinden izole edilmiş Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas monteilii izolatları ile Pseudomonas aeruginosa ATCC 27853 ve ATCC 10145’e karşı ticari olarak satılan deve dikeni (Silybum marianum) sabit yağı, limon (Citrus limon) ve sardunya (Pelargonium graveolens) uçucu yağlarının antibakteriyel aktivitesi karşı agar kuyu difüzyon ve mikrodilüsyon yöntemiyle in vitro olarak değerlendirilmiştir. Test edilen Pseudomonas türlerine deve dikeni sabit yağı, limon ve sardunya uçucu yağlarının inhibisyon zonu oluşturmadığı ve MİK değerlerinin >80 μL/mL olduğu tespit edilmiştir.

Anahtar Kelimeler

• Citrus limon
• Pelargonium graveolens
• Silybum marianum
• Pseudomonas türleri
• Antibakteriyel aktivite

Kaynakça

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