Effects of selenium, vitamin E, and β-carotene administration on fertility of Awassi ewes synchronized for estrus in non-breeding season

Öz

The aim of this study was to evaluate the effect of selenium, β-carotene, and vitamin E administration on fertility of Awassi ewes synchronized in non-breeding season. A total of 80 multiparous Awassi ewes, aged 2 to 6 years were enrolled in the study. Intravaginal sponges containing flugeston acetate (20 mg cronolon) was inserted into vagina and allowed to remain in vagina for nine days. Ewes were randomly divided into two groups. Group I received intramuscular injections of 1 mg sodium selenite, 60 mg vitamin E, 75 mg β-carotene, and 100 mg DL-α-tocopherol acetate at sponge insertion, sponge removal, and 18 days after the introduction of ram. Group II remained as control group and received no treatment. On the removal of the sponges, 500 IU eCG and 0.075 mg D-cloprostenol were injected intramuscularly. Ram was introduced for 1 hr twice a day 24 hours after the sponge removal. Interval between sponge removal and estrus, estrus rates, conception rates, pregnancy rates, lambing rates, and fecundity rates in group I and group II were 45.30±1.71 and 43.94±1.72 hours, 79.1% and 86.6%, 66.66% and 72.41%, 54.05% and 58.33%, 100% and 100%, and 135% and 138%, respectively. There were no statistical differences between the groups (p>0.05). In conclusion, the administration of Se, β-carotene, and vitamin E in estrus synchronization protocols in non-breeding season had no positive effect on the fertility characteristics of Awassi sheep.

Anahtar Kelimeler

• vitamin E
• Sheep
• synchronization
• selenium
• β-carotene

Kaynakça

1. 1. Arechiga, C. F., Vazquez-Flores, S., Ortiz, O., Hernandez-Ceron, J., Porras, A., McDowell, L. R., & Hansen, P. J. (1998). Effect of injection of β-carotene or vitamin E and selenium on fertility of lactating dairy cows. Theriogenology, 50(1), 65-76. https://doi.org/10.1016/S0093-691X(98)00114-9
2. 2. Arellano-Rodriguez, G., Meza-Herrera, C. A., Rodriguez-Martinez, R., Velazquez-Mendez, G., Mellado, M., Salinas, H., Perez-Razo, M. A., & Sanchez, F. (2007). Short-term betacarotene supplementation positively affects ovarian follicular development and ovulation rate in goats. Journal of Applied Animal Research, 32(2), 177-180. https://doi.org/10.1080/09712119.2007.9706872
3. 3. Awawdeh, M. S., Eljarah, A. H., & Ababneh, M. M. (2019). Multiple injections of vitamin E and selenium improved the reproductive performance of estrus-synchronized Awassi ewes. Tropical animal health and production, 51(6), 1421-1426. https://doi.org/10.1007/s11250-019-01826-0
4. 4. Ay, S. S., Kükükaslan, I., Kaya, D., Mülazimoglu, B., Emre, B., Kacar, C., Kalender, H., Fındık, M., Bollwein, H., Riegler, M., Schäfer, S., & Scholbach, J. (2012). The change in luteal blood flow and luteal size after beta carotene and Gnrh injections in early pregnant dairy cows. Kafkas Universitesi Veteriner Fakultesi Dergisi, 18(6), 1035-1041. https://doi.org/10.9775/kvfd.2012.6959
5. 5. Ayaşan, T., & Karakozak, E. (2010). Hayvan beslemede β-karoten kullanılması ve etkileri. Kafkas Universitesi Veteriner Fakultesi Dergis, 16(4), 697-705. https://doi.org/10.9775/kvfd.2010.2008
6. 6. Beytut, E., Kamiloğlu, N. N., & Gökçe, G. (2005). Seasonal variation of vitamin A, E and beta carotene levels in plasma of sheeps and Meadow Hay in the region of Kars and its surrounds. Kafkas Universitesi Veteriner Fakultesi Dergisi, 11(1), 17-24.
7. 7. Ceko, M. J., Hummitzsch, K., Hatzirodos, N., Bonner, W. M., Aitken, J. B., Russell, D. L., Lane, M., Rodgers, R. J., & Harris, H. H. (2015). X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function. Metallomics, 7(1), 71-82. https://doi.org/10.1039/c4mt00228h
8. 8. Çelik, H. A., Avcı, G., Aydın, İ., Bülbül, A., & Bülbül, T. (2009). Effect of β-carotene on ovarium functions and Ovsynch success in repeat breeder cows. Kafkas Universitesi Veteriner Fakultesi Dergisi, 15(1), 87-94. https://doi.org/10.9775/kvfd.2008.82-A

9. 9. De Ondarza, M. B., Wilson, J. W., & Engstrom, M. (2009). Case study: Effect of supplemental β-carotene on yield of milk and milk components and on reproduction of dairy cows. The Professional Animal Scientist, 25(4), 510-516. https://doi.org/10.15232/S1080-7446(15)30742-7
10. 10. El-Shahat, K. H., & Abdel Monem, U. M. (2011). Effects of dietary supplementation with vitamin E and/or selenium on metabolic and reproductive performance of Egyptian Baladi ewes under subtropical conditions. World Applied Sciences Journal, 12(9), 1492-1499.
11. 11. Farahavar, A., Rostami, Z., Alipour, D., & Ahmadi, A. (2020). The effect of pre-breeding vitamin E and selenium injection on reproductive performance, antioxidant status, and progesterone concentration in estrus-synchronized Mehraban ewes. Tropical animal health and production, 52(4), 1779-1786. https://doi.org/10.1007/s11250-019-02183-8
12. 12. Gordon, I. (1997). Controlled Reproduction in sheep and goats, Universty College Dublin Ireland.
13. 13. Gore, D. L. M., & Lehloenya, K. C. (2020). β-carotene supplementation increases progesterone concentration and glutathione peroxidase activity following alternative progesterone primed oestrous synchronization protocol in goats. American Journal of Animal and Veterinary Sciences , 15 (3): 211-219. https://doi.org/10.3844/ajavsp.2020.211.219
14. 14. Haliloğlu, S., Baspinar, N., Serpek, B., Erdem, H., & Bulut, Z. (2002). Vitamin A and β‐carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reproduction in Domestic Animals, 37(2), 96-99. https://doi.org/10.1046/j.1439-0531.2002.00338.x
15. 15. Hostetler, C. E., Kincaid, R. L., & Mirando, M. A. (2003). The role of essential trace elements in embryonic and fetal development in livestock. The Veterinary Journal, 166(2), 125-139. https://doi.org/10.1016/S1090-0233(02)00310-6
16. 16. Karagiannidis, A., Varsakeli, S., Karatzas, G., & Brozos, C. (2001). Effect of time of artificial insemination on fertility of progestagen and PMSG treated indigenous Greek ewes, during non-breeding season. Small Ruminant Research, 39(1), 67-71. https://doi.org/10.1016/S0921-4488(00)00170-X
17. 17. Kott, R. W., Thomas, V. M., Hatfield, P. G., Evans, T., & Davis, K. C. (1998). Effects of dietary vitamin E supplementation during late pregnancy on lamb mortality and ewe productivity. Journal of the American Veterinary Medical Association, 212(7), 997-1000.
18. 18. Koyuncu, M., & Yerlikaya, H. (2007). Effect of selenium-vitamin E injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science, 37(4), 233-236.
19. 19. Köse, M., Kırbaș, M., Dursun, Ș., & Bayrıl, T. (2013). The effect of injections of β-carotene or vitamin E+ Selenium on fertility in ewes in anestrus season. Yüzüncü yıl Üniversitesi Veteriner Fakültesi Dergisi, 24(2), 83-86.
20. 20. Kuru, M., Sogukpinar, O., Makav. M., & Cetin N. (2017). Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Medycyna weterynaryjna, 73(8): 479-482. https://doi.org/10.21521/mw.5758
21. 21. Liu, S., Masters, D., Ferguson, M., & Thompson, A. (2014). Vitamin E status and reproduction in sheep: potential implications for Australian sheep production. Animal Production Science, 54(6), 694-714. https://doi.org/10.1071/AN13243
22. 22. Mehdi, Y., & Dufrasne, I. (2016). Selenium in cattle: a review. Molecules, 21(4), 545. https://doi.org/10.3390/molecules21040545
23. 23. Moss, G. E., Adams, T. E., Niswender, G. D., & Nett, T. M. (1980). Effects of parturition and suckling on concentrations of pituitary gonadotropins, hypothalamic GnRH and pituitary responsiveness to GnRH in ewes. Journal of Animal Science, 50(3), 496-502. https://doi.org/10.2527/jas1980.503496x
24. 24. Putnam, M. E., & Comben, N. (1987). Vitamin E. Veterinary Record, 121, 541-545.
25. 25. Sánchez, J., Jiménez, A., Regodón, S., & Andrés, S. (2008). Inhibitory effect of selenium supplementation on the reproductive performance in synchronized merino sheep at range conditions in a selenium‐deficient area. Reproduction in Domestic Animals, 43(3), 328-332. https://doi.org/10.1111/j.1439-0531.2007.00904.x
26. 26. Sarıbay, M. K., & Erdem, H. (2007). Kovunlarda real-time ultrasonografi ile embrivonik ölümlerin insidansının belirlenmesi. Eurasian Journal of Veterinary Sciences, 23(3-4), 19-25.
27. 27. Scaramuzzi, R. J., Campbell, B. K., Downing, J. A., Kendall, N. R., Khalid, M., Muñoz-Gutiérrez, M., & Somchit, A. (2006). A review of the effects of supplementary nutrition in the ewe on the concentrations of reproductive and metabolic hormones and the mechanisms that regulate folliculogenesis and ovulation rate. Reproduction Nutrition Development, 46(4), 339-354. https://doi.org/10.1051/rnd:2006016
28. 28. Segerson, E. C., Gunsett, F. C., & Getz, W. R. (1986). Selenium-vitamin E supplementation and production efficiency in ewes marginally deficient in selenium. Livestock Production Science, 14(2), 149-159. https://doi.org/10.1016/0301-6226(86)90004-7
29. 29. Trojacanec, S., Boboš, S., & Pajić, M. (2012). Influence of β-carotene and vitamin A supplementation on the ovarian activity of dairy cows with chronic fertility impairment. Veterinarski Arhiv, 82(6), 567-575.
30. 30. Van Metre, D. C., & Callan, R. J. (2001). Selenium and vitamin E. Veterinary Clinics of North America: Food Animal Practice, 17(2), 373-402. https://doi.org/10.1016/S0749-0720(15)30034-7
31. 31. Van Niekerk, F. E., Cloete, S. W. P., Heine, E. W. P., Van der Merwe, G. D., Wellington, A., Du Plessis, SS & Bekker, D. (1996). The effect of selenium supplementation during the early post-mating period on embryonic survival in sheep. Journal of the South African Veterinary Association, 67(4), 209-213. https://hdl.handle.net/10520/AJA00382809_1685
32. 32. Wang, J. Y., Hafi, C. B., Owen, F. G., & Larson, L. L. (1987). Effect of beta-carotene supplementation on periparturient health and reproduction of Holstein cows. Animal Reproduction Science, 15(1-2), 139-144. https://doi.org/10.1016/0378-4320(87)90013-3
33. 33. Weiss, W. P. (1998). Requirements of fat-soluble vitamins for dairy cows: a review. Journal of Dairy Science, 81(9), 2493-2501. https://doi.org/10.3168/jds.S0022-0302(98)70141-9
34. 34. Wichtel, J. J., Freeman, D. A., Craigie, A. L., Varela-Alvarez, H., & Williamson, N. B. (1996). Alpha-tocopherol, selenium and polyunsaturated fatty acid concentrations in the serum and feed of spring-calving dairy heifers. New Zealand Veterinary Journal, 44(1), 15-21. https://doi.org/10.1080/00480169.1996.35925
35. 35. Wildeus, S. (2000). Current concepts in synchronization of estrus: Sheep and goats. Journal of Animal Science, 77 (suppl_E), 1-14. https://doi.org/10.2527/jas2000.00218812007700ES0040x
36. 36. Yıldız, A., Balıkcı, E., & Gürdoğan, F. (2015). Effect of injection of vitamin E and selenium administered immediately before the Ovsynch synchronization on conception rate, antioxidant activity and progesterone levels in dairy cows. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 29(3), 183-186.