Investigation of the effects of feline coronavirus (FCoV) infection on calcium metabolizm in cats.

Öz

Çalışmada feline coronavirus (FCoV) ile enfekte kedilerde enfeksiyonun kan kalsiyum seviyesi ve Ca metabolizmasını düzenleyen parametreler üzerine etkisinin belirlenmesi amaçlanmıştır. Çalışmada FİP pozitif, feline immunodeficiency virüs (FIV) ve feline leucoma virüs (FeLV) negatif kuru (n=10) ve yaş form (n=10) krıterlerini taşıyan 20 adet FİP li kedi kullanılmıştır. Ayrıca 10 adet FİP, FIV, FeLV negatif sağlıklı kedi çalışmaya kontrol grubu olarak dahil edilmiştir. Tüm kedilerin serum örnekleri toplanarak bu örneklerde kedi spesifik ELISA test kitleri kullanılarak paratiroid hormon (PTH), paratiroid hormon benzeri protein (PTHrP), kalsitonin ve vitamin D3 (Vit D3) düzeyleri belirlendi. Ayrıca tüm serum örneklerinde otomatik biyokimya cihazı kullanılarak kalsiyum (Ca), magnezyum (Mg) ve Fosfor (P) ölçümleri yapıldı. Yapılan analizler sonucunda FİP’li kedilerin PTH (p<0,01), PTHrP (p<0,001) ve P (p<0,01) düzeyleri kontrol grubu kedilerin değerlerine göre önemli düzeyde yüksek olduğu belirlendi. Bununla birlikte FİP’li kedilerin kalsitonin ve vitamin D3, Ca seviyeleri kontrol grubuna göre yüksek olmakla birlikte iki grup arasında istatistiksel olarak herhangi bir farkın olmadığı anlaşıldı. Anahtar Kelimeler: biyokimya, feline infeksiyöz peritonitis (FİP), kedi. ABSTRACT The aims of the study were to determine the effects of feline coronavirus (FCoV) infection on blood Ca levels and Ca metabolism regulatory parameters in cats. In the study, twenty cats with clinical symthoms of feline infectious peritonitis (FIP) and positive to FCoV were used. They were negative to feline immunodeficiency virus (FIV) and feline leucoma virus (FeLV). These cats were divided into two groups equally as dry (n=10) and wet form (n=10) of FIP. Ten cats clinically healthy and negative for all test were also used as control group. Serum samples were collected from all the cats and they were used to analyse parathyroid hormone (PTH), parathyroid hormone-realted protein (PTHrP), calcitonin and vitamin D3 (Vit D3), using feline specific ELISA test kits. Furthermore, Calcium (Ca), magnesium, (Mg) and phosphorus (P) levels were also measured in these serum samples. In the sudy, PTH (p<0,01), PTHrP (p<0,001) and P (p<0,01) levels were significantly higher in cats with FIP than those of control group. Additionally, calcitonin, vitamin D3 and Ca levels were found to be high in cats with FIP compared to those of control cats, but there were no statistically significance between these groups.

Anahtar Kelimeler

• feline infeksiyöz peritonitis (FİP)
• kedi.
• biyokimya

Kedilerde feline coronavirus (FCoV) enfeksiyonunun kalsiyum metabolizması üzerindeki etkisinin araştırılması

Öz

ÖZ Çalışmada feline coronavirus (FCoV) ile enfekte kedilerde enfeksiyonun kan kalsiyum seviyesi ve Ca metabolizmasını düzenleyen parametreler üzerine etkisinin belirlenmesi amaçlanmıştır. Çalışmada FİP pozitif, feline immunodeficiency virüs (FIV) ve feline leucoma virüs (FeLV) negatif kuru (n=10) ve yaş form (n=10) krıterlerini taşıyan 20 adet FİP li kedi kullanılmıştır. Ayrıca 10 adet FİP, FIV, FeLV negatif sağlıklı kedi çalışmaya kontrol grubu olarak dahil edilmiştir. Tüm kedilerin serum örnekleri toplanarak bu örneklerde kedi spesifik ELISA test kitleri kullanılarak paratiroid hormon (PTH), paratiroid hormon benzeri protein (PTHrP), kalsitonin ve vitamin D3 (Vit D3) düzeyleri belirlendi. Ayrıca tüm serum örneklerinde otomatik biyokimya cihazı kullanılarak kalsiyum (Ca), magnezyum (Mg) ve Fosfor (P) ölçümleri yapıldı. Yapılan analizler sonucunda FİP’li kedilerin PTH (p<0,01), PTHrP (p<0,001) ve P (p<0,01) düzeyleri kontrol grubu kedilerin değerlerine göre önemli düzeyde yüksek olduğu belirlendi. Bununla birlikte FİP’li kedilerin kalsitonin ve vitamin D3, Ca seviyeleri kontrol grubuna göre yüksek olmakla birlikte iki grup arasında istatistiksel olarak herhangi bir farkın olmadığı anlaşıldı. Anahtar Kelimeler: biyokimya, feline infeksiyöz peritonitis (FİP), kedi. ABSTRACT The aims of the study were to determine the effects of feline coronavirus (FCoV) infection on blood Ca levels and Ca metabolism regulatory parameters in cats. In the study, twenty cats with clinical symthoms of feline infectious peritonitis (FIP) and positive to FCoV were used. They were negative to feline immunodeficiency virus (FIV) and feline leucoma virus (FeLV). These cats were divided into two groups equally as dry (n=10) and wet form (n=10) of FIP. Ten cats clinically healthy and negative for all test were also used as control group. Serum samples were collected from all the cats and they were used to analyse parathyroid hormone (PTH), parathyroid hormone-realted protein (PTHrP), calcitonin and vitamin D3 (Vit D3), using feline specific ELISA test kits. Furthermore, Calcium (Ca), magnesium, (Mg) and phosphorus (P) levels were also measured in these serum samples. In the sudy, PTH (p<0,01), PTHrP (p<0,001) and P (p<0,01) levels were significantly higher in cats with FIP than those of control group. Additionally, calcitonin, vitamin D3 and Ca levels were found to be high in cats with FIP compared to those of control cats, but there were no statistically significance between these groups. Key Words: biochemistry, cat, feline infectious peritonitis (FIP).

Anahtar Kelimeler

• biyokimya
• feline infeksiyöz peritonitis (FİP)
• kedi.

Kaynakça

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