The ameliorative effect of Acetylsalicylic acid plus Ascorbic acid against renal injury in Corn Syrup-fed rats

Öz

Dietary consumption of commercially prepared (often through processing) corn syrup can activate reactive oxygen species and the inflammatory pathways observed in kidney damage in humans and experimental animals. This study was designed to assess the effects of antioxidant properties of acetylsalicylic (ASA) and ascorbic acid (AA) on corn syrup (CS) induced renal injury. Forty male Sprague–Dawley rats (14–16 weeks) were randomly classified into 5 groups, each containing 8 rats: control group (C), CS group, CS+ASA group (ASA-10 mg/kg/day/po), CS+AA group (AA-200 mg/kg/day/po) and CS+ASA+AA group (combination treatment with ASA and AA doses). At the end of the experiment, blood and tissue samples were collected for biochemical, histopathological and immunohistochemical examinations. Malondialdehyde (p<0.001) levels were increased in the CS group compared with control group and were decreased in the ASA, AA and ASA+AA treated groups (p≤0.001 for all) compared with CS group. Catalase activities were decreased in the CS group (p≤0.001) compared with the control group (p<0.001) and significantly increased in AS+AA-treated group (p<0.001) compared with the CS group. Significant histopathological changes including tubular vacuoler degeneration, tubular dilatation, cortical and medullar haemorrhage, mononuclear cell infiltration, and increased inducible nitric oxide synthase and tumor necrosis factor-α staining were observed in the CS group. Combination theraphy reversed all these changes. The administration of ASA and AA for the treatment of kidney damage due to corn syrup consumption provides an ideal target for improving oxidative stress and potential therapeutic treatments.

Anahtar Kelimeler

• Acetylsalicylic acid
• Ascorbic acid
• Corn syrup
• Renal injury

Kaynakça

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