Radyoiodin (131I) Uygulanan Ratlarda Karaciğerdeki Histopatolojik Bulgular ve Hepatik Satellate Hücrelerde Artış

Yıl 2017, Cilt: 2 Sayı: 2, 117 - 123, 29.12.2017

Rahşan Yılmaz , Nihat Yumuşak , Hasan İkbal Atılgan Gökhan Koca , Meliha Korkmaz

https://doi.org/10.24880/maeuvfd.349014

Öz

Radyoaktif iyot (¹³¹I) hipertiroidizm ve tiroid kanserlerinin
tanı ve tedavisi amacıyla kullanılan bir radyonükliddir. Bu çalışma ile ratlarda
radyoiodinden (¹³¹I) kaynaklanan karaciğer hasarının histopatolojik ve
immunohistokimyasal olarak değerlendirilmesi amaçlandı. Çalışmada 20 adet dişi
Wistar Albino rat kullanıldı ve bunlar rastgele hiçbir işleme tabi tutulmayan
kontrol grubu (n=10) ve ¹³¹I uygulama grubu (n=10)  şeklinde ikiye ayrıldı. Üçüncü ayın sonunda
nekropsileri yapılan ratların karaciğer dokuları rutin doku takibine alındı. Karaciğerin
histopatolojik incelemesinde ¹³¹I grubunda kapsula altında granulom oluşumlarına rastlandı. İmmunohistokimyasal
boyamada ¹³¹I grubunda hepatik satellate hücrelerin (HPCs),
yoğunluğunu gösteren GFAP boyamalarının kontrol grubundan daha yüksek bir
değere sahip olduğu görüldü.

Anahtar Kelimeler

131I, histopatoloji, karaciğer, HPCs, GFAP, immunohistokimya

Hystopathological Findings and Hepatic Satellite Cells İncrease in the Liver of the Rats Applied Radionin (131I)

Öz

Radioactive iodine (131I) is a radionuclide used for the diagnosis and treatment of thyroid
cancers and hyperthyroidism. This study was aimed to evaluation by histopathological and
immunohistochemical of hepatic damage caused by radioiodine (131I) in the rat. Twenty
female Wistar Albino rats were used in the study and they were randomly divided into two
groups as control group (n = 10) and 131I application group (n = 10). At the end of the third
month, the liver tissues were taken of rats at the necropsy, the tissue specimens underwent
routine tissue processing. Histopathological examination was observed granuloma formation
in the liver in 131I group. Immunohistochemical staining the GFAP was showed in the 131I
group the higher than in the control group, that showed density of the hepatic satellite cells
(HPCs). 

Anahtar Kelimeler

131I, histopathology, liver, HPCs, GFAP, immunohistochemistry, rat

Kaynakça

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