The effect of Calcitriol 1,25 (OH)2 - D3 on osteoblast-like cell proliferation during in vitro cultivation

Yıl 2020, Cilt: 5 Sayı: 1, 11 - 17, 30.04.2020

Muhamed Katica Filiz Tepekoy

https://doi.org/10.24880/maeuvfd.653000

Cited By: 2

Öz

Derived from mesenchymal stem cells (MSCs) under the influence of various local factors and growth hormones, osteoblasts are one of the leading components of the bone tissue. 1,25-dihydroxyvitamin D3 (calcitriol), an active form of vitamin D, plays an important role in retaining calcium and phosphate homeostasis within the body. Since vitamin D is also known to have effects on cell proliferation, our study aimed to investigate the effects of different concentrations of calcitriol on the proliferation of in vitro cultured osteoblast-like cells from the bone marrow of 90-95 days old young adult rats of both sexes. Calcitriol was applied at six (6) different concentrations (nM): 0.1, 1, 10, 20, 40 i 60. Each concentration was tested four times, counting the cells after 24 and 48 hours. After 24 and 48 hours treatment, the most effective dose of calcitriol for cell proliferation was 0.1 nM for both males and females. The greatest reduction in the frequency of OB-like cells from both sexes after treatment of 24 and 48 hours, was a 60 nM calcitriol concentration. Higher proliferative values of osteoblast-like cells were obtained in males compared to those obtained in females. Thus, the results of the current study reveal that calcitriol treatment induces the proliferation of osteoblast-like cells in a dose-dependent manner and this effect shows difference in cells from different genders.

Anahtar Kelimeler

osteoblast like cells, rat, calcitriol, in vitro cultivation

Kaynakça

• 1. Lagumdžija A., The effects of some peptide hormones on osteoblast-like cells- with specific fokus on pxitocin and vasopresin. Doctoral thesis. The Endocrine and Diabetes Unit Department Institutet, Sweden, 2005.
• 2. Olsen BR., Reginato AM, Wang W. Bone development. Annu Rev Cell Dev Biol. 2000; 16: 191-220.
• 3. Cui J, Zhang W, Huang E, Wang J, Liao J, Li R., et al. Bmp9-induced osteoblastic differentiation requires functional notch signaling in mesenchymal stem cells. Lab Invest. 2019; 99(1): 58-71.
• 4. Komori T. Regulation of osteoblast differentiation by transcription factors. J Cell Biochem. 2006; 99: 1233-39.
• 5. Katica M. Effects of vitamin D3 and calcium on the establishment of primary osteoblast culture. Master's thesis. University of Sarajevo, Faculty of Sciences. Bosnia and Herzegovina, 2007.
• 6. Arumugam B, Vishal M, Shreya S, Malavika D, Rajpriya V, He Z, et al. Parathyroid hormone-stimulation of runx2 during osteoblast differentiation via the regulation of lnc-supt3h-1:16 (runx2-as1:32) and mir-6797-5p. Biochimie. 2019; 158(10): 43-52.
• 7. Anusaksathien O & Giannobile WV. Growth factor delivery to re-engineer periodontal tissues. Curr Pharm Biotechnol. 2002; 3(2): 129-139.
• 8. Minghetti PP & Norman AW. 1,25(oh)2-vitamin d3 receptors: Gene regulation and genetic circuitry. FASEB J. 1988; 2(15): 3043-53.
• 9. Ćupić V, Muminović M, Kobal S, Velev R. Pharmacology for students of faculties of veterinary medicine, 2nd ed. Belgrade: Naučna, KMD; 2014. p.141-146; 429-432.
• 10. Nagpal S, Na S, Rathnachalam R. Noncalcemic actions of vitamin d receptor ligands. Endocr Rev. 2005; 26(5): 662-67.
• 11. Chiu LH, Lai WF, Chang SF, Wong CC, Fan CY, Fang CL, et al. The effect of type ii collagen on msc osteogenic differentiation and bone defect repair. Biomaterials. 2014; 35(9): 2680-91.
• 12. Posa F, Di Benedetto A, Cavalcanti-Adam EA, Colaianni G, Porro C, Trotta T. Vitamin d promotes msc osteogenic differentiation stimulating cell adhesion and alphavbeta3 expression. Stem Cells Int. 2018a; 6958713: 1-9.
• 13. Liu P, Oyajobi BO, Russell RG, Scutt A. Regulation of osteogenic differentiation of human bone marrow stromal cells: Interaction between transforming growth factor-beta and 1,25(oh)(2) vitamin d(3) in vitro. Calcif Tissue Int. 1999; 65: 173-80.
• 14. Bolland MJ & Grey A. A case study of discordant overlapping meta-analyses: Vitamin d supplements and fracture. PLoS One. 2014; 9(12): e115934.
• 15. Macdonald HM, Reid IR, Gamble GD, Fraser WD, Tang JC, Wood AD. 25-hydroxyvitamin d threshold for the effects of vitamin d supplements on bone density: Secondary analysis of a randomized controlled trial. J Bone Miner Res. 2018; 33(8): 1464-69.
• 16. Reid IR, Bolland MJ, Grey A. Effects of vitamin d supplements on bone mineral density: A systematic review and meta-analysis. Lancet. 2014; 383: 146-55.
• 17. Maniatopoulos C, Sodek J, Melcher AH. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res. 1988; 254(2): 317-30.
• 18. Paine A, Woeller CF, Zhang H, de la Luz Garcia-Hernandez M, Huertas N, Xing L, et al. Thy1 is a positive regulator of osteoblast differentiation and modulates bone homeostasis in obese mice. FASEB J. 2018.; 32(6): 3174-83.
• 19. Buettmann EG, McKenzie JA, Migotsky N, Sykes DA, Hu P, Yoneda S, et al. Vegfa from early osteoblast lineage cells (osterix+) is required in mice for fracture healing. J Bone Miner Res. 2019; 34(1): 1690-706.
• 20. Davis JM. Basic cell culture – a practical approach. 1st ed. Oxford University, Oxford: IRL Press; 1996. p. 80-90.
• 21. Wong GL, Luben RA, Cohn DV. 1,25-dihydroxycholecalciferol and parathormone: Effects on isolated osteoclast-like and osteoblast-like cells. Science, 1977; 197: 663-65.
• 22. Wlodarski K H. Properties and origin of osteoblasts. Clin Orthop Relat Res. 1990; 276-93.
• 23. Viereck V, Siggelkow H, Tauber S, Raddatz D, Schutze N, Hufner M. Differential regulation of cbfa1/runx2 and osteocalcin gene expression by vitamin-d3, dexamethasone, and local growth factors in primary human osteoblasts. J Cell Biochem. 2002; 86: 348-56.
• 24. Logovskaya LV, Bukharova TB, Volkov AV, Vikhrova EB, Makhnach OV, Goldshtein DV. Induction of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue. Bull Exp Biol Med. 2013; 155(1): 145-50.
• 25. Posa F, Di Benedetto A, Colaianni G, Cavalcanti-Adam EA, Brunetti G, Porro C, et al. Vitamin d effects on osteoblastic differentiation of mesenchymal stem cells from dental tissues. Stem Cells Int. 2016; 2016: 9150819.
• 26. Bellows CG, Reimers SM, Heersche JN. Expression of mrnas for type-i collagen, bone sialoprotein, osteocalcin, and osteopontin at different stages of osteoblastic differentiation and their regulation by 1,25 dihydroxyvitamin d3. Cell Tissue Res. 1999; 297(2): 249-59.
• 27. Woeckel VJ., van der Eerden BC, Schreuders-Koedam M, Eijken M, Van Leeuwen JP. 1alpha,25-dihydroxyvitamin d3 stimulates activin a production to fine-tune osteoblast-induced mineralization. J Cell Physiol. 2013; 228(11): 2167-74.
• 28. van de Peppel J & van Leeuwen JP. Vitamin d and gene networks in human osteoblasts. Front Physiol. 2014; 5: 137.
• 29. Schneider GB, Zaharias R, Stanford C. Osteoblast integrin adhesion and signaling regulate mineralization. J Dent Res. 2001; 80(6): 1540-4.
• 30. Hamidouche Z, Fromigue O, Ringe J, Haupl T, Vaudin P, Pages JC, et al. Priming integrin alpha5 promotes human mesenchymal stromal cell osteoblast differentiation and osteogenesis. Proc Natl Acad Sci U S A. 2009; 106(44): 18587-91.
• 31. Wang YK & Chen CS. Cell adhesion and mechanical stimulation in the regulation of mesenchymal stem cell differentiation. J Cell Mol Med. 2013; 17(7): 823-32.
• 32. Raz P, Lohmann CH, Turner J, Wang L, Poythress N, Blanchard C, et al. 1alpha, 25(oh)2d3 regulation of integrin expression is substrate dependent. J Biomed Mater Res A. 2004; 71: 217-25.
• 33. Posa F, Di Benedetto A, Cavalcanti-Adam EA, Colaianni G, Porro C, Trotta T, et al. Vitamin d promotes msc osteogenic differentiation stimulating cell adhesion and alphavbeta3 expression. Stem Cells Int, 2018b; 6958713:1-9.
• 34. Tosi LL, Boyan BD, Boskey AL. Does sex matter in musculoskeletal health? The influence of sex and gender on musculoskeletal health. J Bone Joint Surg Am. 2005; 87: 1631-47.
• 35. Jochems C, Islander U, Erlandsson M, Engdahl C, Lagerquist M, Gjertsson I, et al. Role of endogenous and exogenous female sex hormones in arthritis and osteoporosis development in b10.Q-ncf1*/* mice with collagen-induced chronic arthritis. BMC Musculoskelet Disord. 2010; 11: 284.
• 36. D'Amelio P, Rossi P, Isaia G, Lollino N, Castoldi F, Girardo M, et al. Bone mineral density and singh index predict bone mechanical properties of human femur. Connect Tissue Res. 2008; 49: 99-104.
• 37. Olivares-Navarrete R, Hyzy SL, Boyan BD, Schwartz Z. Regulation of osteoblast differentiation by acid-etched and/or grit-blasted titanium substrate topography is enhanced by 1,25(oh)2d3 in a sex-dependent manner. Biomed Res Int. 2015; 2015: 365014.
• 38. Berger MB, Cohen DJ, Olivares-Navarrete R, Williams JK, Cochran DL, Boyan BD, et al. Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces. Biol Sex Differ. 2018; 9(1): 30.