The effects of ethanol extract of Punica granatum L. peel on the testis damage induced by diabetes in rats

Yıl 2023, Cilt: 8 Sayı: 1, 1 - 7, 30.04.2023

Ali Ömür Betül Apaydın Yıldırım Serkan Yıldırım Serkan Ali Akarsu

https://doi.org/10.24880/maeuvfd.1075862

Öz

In this study, it was aimed to determine the effect of ethanol extract of Punica granatum L. peel (PGE) on oxidative stress and histopathological values in blood and testis tissue in rats with diabetes. In present study used twenty-eight male Sprague Dawley rats were randomly divided into four equal groups containing of seven rats per group. Group 1; peros physiological saline to the rat, Group 2; STZ 60 mg/kg/IP single dose, Group 3; PGE 10 mg/kg/20days/peros, Group 4; STZ as 60 mg/kg/IP + PGE 10 mg/kg/20days/peros. After the end of the experimental procedure, the rats were sacrificed, blood and testicular tissues were taken, and biochemical and histopathological examinations were performed. The administration of PGE was shown that the activities of CAT, SOD, GPx and GSH increased and the levels of MDA decreased in diabetic rats. Oral administration of PGE reduced the levels of LPO and improved the antioxidant activity in plasma and testis tissues while compared with the Groups 4. Histopathological examination of testicular tissues in the Group 2 is revealed edema in the intertubular spaces, thinning of the tubule walls due to diminished spermatocytes in the walls of the seminiferous tubules and severe degenerative and necrotic changes in spermatocytes. These changes were found to be very mild in Group 4. According to immunohistochemical findings, in Group 2, caspase 3 expression was intensely expressed in spermatocytes. As a result, it was observed that Punica granatum L. peel extract strengthened antioxidant defense and reduced oxidative stress in diabetic rats.

Anahtar Kelimeler

Diabetes, Oxidative stress, Rat, Streptozotocin, Punica Granatum L. peel

Kaynakça

• 1. Akhtar, S., Ismail, T., Fraternale, D., Sestili, P. (2015). Pomegranate peel and peel extracts. Chemistry and food features, Food Chem, 174, 417-25.
• 2. Aydilek, N., Aksakal, M. (2003). Effect of testosterone on liver antioxidant system in rabbits. Journal of Yuzuncu Yıl University Faculty of Veterinary Medicine, 14(2), 22-5.
• 3. Ball, C. R. (1966). Estimation and identification of thiols in rat spleen after cysteine or glutathione treatment, pelevance to protection against nitrojen mustards. Biochemical Pharmacology, 15, 809-16.
• 4. Baker, H. W. (1998). Reproductive effects of nontesticular illness. Endocrinology and Metabolism Clinics of North America, 27, 831–50.
• 5. Baynes JW, Thorpe SR. (1999). Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes, 48(1), 1-9.
• 6. Benzie, I. F., Wachtel-Galor S. Herbal medicine: biomolecular and clinical aspects (2011).
• 7. Boroujeni, M. B., Shahrokhi, S. S., Birjandi, M., Abbaszadeh, A., Beyranvand, F., Hamoleh, S., ... & Gholami, M. (2017). Effects of pomegranate peel extract on histopathology, testosterone levels and sperm of testicular torsion–detorsion induced in adult Wistar rats. Journal of Complementary and Integrative Medicine, 14(4).
• 8. Cai, L., Chen, S., Evans, T., Deng, D. X., Mukherjee, K., & Chakrabarti, S. (2000). Apoptotic germ-cell death and testicular damage in experimental diabetes: prevention by endothelin antagonism. Urological research, 28(5), 342-347.
• 9. Cheeseman, K. H., Slater, T. F. (1993). An introduction to free radical biochemistry. British Medical Bulletin, 49(3), 481-93.
• 10. Çakmak, B. (2003). Istanbul University Faculty of Veterinary Medicine. NRA Bulletin, 28.
• 11. Dkhil, M. A., Al-Quraishy, S., Moneim, A. E. A. (2013). Effect of pomegranate (Punica granatum L.) juice and methanolic peel extract on testis of male rats. Pakistan Journal of Zoology, 45(5), 1343-9.
• 12. Doostan, F., Vafafar, R., Zakeri-Milani, P., Pouri, A., Afshar, R. A., & Abbasi, M. M. (2017). Effects of pomegranate (Punica granatum L.) seed and peel methanolic extracts on oxidative stress and lipid profile changes induced by methotrexate in rats. Advanced pharmaceutical bulletin, 7(2), 269.
• 13. Erbaş, O. (2015). Experimental diabetes models. FNG & Science Medical Journal, 1(1), 40-2.
• 14. Fernandez, V., Videla, L. A. (1981). Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of varios tissues of the rat. Experientia, 37, 392-4.
• 15. Fischer, U. A., Carle, R., Kammerer, D. R. (2011). Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MSn. Food chemistry 127(2), 807-21.
• 16. Goth, L. (1991). A simple method for determenation of serum catalase activity and revision of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196, 143-52.
• 17. Henning, S. M., Yang, J., Lee, R. P., Huang, J., Hsu, M., Thames, G., ... & Li, Z. (2019). Pomegranate juice and extract consumption increases the resistance to UVB-induced erythema and changes the skin microbiome in healthy women: a randomized controlled trial. Scientific reports, 9(1), 1-11.
• 18. Ibrahim, S. E. M. (2018). Protective effects of pomegranate (Punica Granatum) peels on the pituitary gonadal hormonal axis of streptozotocin-induced diabetes in adult male albino rats. Al-Azhar Medical Journal, 47(2), 435-48.
• 19. Khaneshi, F., Nasrolahi, O., Azizi, S., Nejati, V. (2013). Sesame effects on testicular damage in streptozotocin-induced diabetes rats. Avicenna Journal of Phytomedicine, 3(4), 347-55.
• 20. Lenzen, S. (2008). The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia, 51, 216-26.
• 21. Longtin, R. (2003). The pomegranate: nature’s power fruit? Journal of the National Cancer Institute, 95(5), 346-8.
• 22. Lydia, K. K., Charles, I. M., Caleb, O. O., Collins, K. K., Benson, G. M., & Paul, N. W. (2019). Effectiveness of pomegranate (Punica Granatum L.) fruit extract on the sexual function in rats. Journal of Microbial and Biochemical Technology, 11(424), 1-6.
• 23. Mahmoud, E. F., Mahmoud, M. F. (2017). Effect of pomegranate peel extract on submandibular salivary glands of streptozotocin induced diabetes in rats: histological, immunohistochemical and ultrastructural study. Journal of Advances in Biology & Biotechnology,13(3), 1-15.
• 24. Matkovics, B., Szabó, L., Sz Varga, I. (1988). Determination of enzyme activities in lipid peroxidation and glutathione pathways (in Hungarian), Laboratóriumi Diagnosztika, 15, 248-50.
• 25. Marti, E., Marti, J. I., Muiño‐Blanco, T., & Cebrián‐Pérez, J. A. (2008). Effect of the cryopreservation process on the activity and immunolocalization of antioxidant enzymes in ram spermatozoa. Journal of Andrology, 29(4), 459-467.
• 26. Minisy, F. M., Shawki, H. H., El Omri, A., Massoud, A. A., Omara, E. A., Metwally, F. G., ... & Oishi, H. (2020). Pomegranate seeds extract possesses a protective effect against tramadol-induced Testicular toxicity in experimental rats. BioMed research international.
• 27. Mythili, M. D., Vyas, R., Akila, G., & Gunasekaran, S. (2004). Effect of streptozotocin on the ultrastructure of rat pancreatic islets. Microscopy research and technique, 63(5), 274-281.
• 28. Newman, R. A., Lansky, E. P. (2007). Pomegranate: The Most Medicinal Fruit. Basic Health Publications 1-10. 29. Placer, Z. A., Cushman, L. L., Johnson, B. C. (1966). Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Analytical Biochemistry, 16, 359-64.
• 30. Scarano, W. R., Messias, A. G., Oliva, S. U., Klinefelter, G. R., & Kempinas, W. G. (2006). Sexual behaviour, sperm quantity and quality after short‐term streptozotocin‐induced hyperglycaemia in rats. International journal of andrology, 29(4), 482-488.
• 31. Sun, Y., Oberley, L. W., Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34, 497-500.
• 32. Tayel, A. A., El‐Tras, W. F. (2010). Anticandidal activity of pomegranate peel extract aerosol as an applicable sanitizing method. Mycoses 53(2), 117-22.
• 33. Tanaka, T., Nonaka, G., Nishioka, I. (1986). Tannisn and Related Compounds. XLI. Isolation and Characterization of Novel Ellagitannins of Punica granatum L, Chemical and Pharmaceutical Bulletin, 34(2), 656-63.
• 34. Tietze, F. (1969). Enzymic method for quantitavite determination of nanogram amounts of total and oxidized glutathione. Analytical Biochemistry, 27, 502-22.
• 35. Yoshioka, T., Kawada, K., Shimada, T., & Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. American journal of obstetrics and gynecology, 135(3), 372-376.