DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvas (Nematoda) in Sander Lucioperca from Lake Eğirdir

Öz

In this study, 926-954 bp partially distinct nucleotide sequences which belong to the 18S and 28SrDNA gene loci from 3 Eustrongylides excisus samples were identified as identical and registered in GenBank (OP480437-39). Nucleotide values in 18S and 28S rDNA gene sequences of Eustrongylides excisus samples were defined respectively: 26.94% 27.26 A; 26.78%-26.84% T; 28.30-28.62% G; 17.39-17.92% C. According to the sequence dataset distance matrix obtained by the Pairwise comparison method, there was a complete agreement (0%) between the Eustrongylides excisus isolates identified in this study (OP480437-39) and the Eustrongylides excisus isolate registered in the GenBank (MK007967, MT415236, MK545494).The Eustrongylides excisus isolates were collected in the same cluster in Maximum likelihood filogram analysis. The internal transcribed spacer (ITS) region gene sequence results of the isolates confirmed the taxonomic position of Eustrongylides excisus, which was defined according to its anatomical and morphological features. More, 18S and 28S rDNA gene sequences were defined for the first time in this study on 3 samples of Eustrongylides excisus species in Lake Eğirdir, and contributions were made to the determination of genetic characteristics of the species.

Anahtar Kelimeler

• Eustrongylides excisus
• DNA sequencing
• Sander lucioperca

Proje Numarası

21.Fen.Bil.25

DNA Sequence Based Molecular Identification of Eustrongylides excisus Larvae (Nematoda) in Sander Luciopercafrom Lake Eğirdir

Öz

Bu çalışmada, 3Eustrongylides excisus örneğinden 18S ve 28SrDNA gen lokuslarına ait 926-954 bp kısmi belirgin nükleotid dizileri özdeş olarak tanımlanmış ve GenBank’ta kayıt altına alınmıştır (OP480437-39). Eustrongylides excisus örneklerinin 18S ve 28SrDNA gen dizilerindeki nükleotid değerleri tanımlanmıştır: % 26,94-27,26 A; %26,78-26,84 T; % 28,30-28,62 G; % 17,39-17,92 C. Pairwise comparison metodu ile elde edilen dizi veri kümesi mesafe matrisine göre, bu çalışmada tanımlanan Eustrongylides excisus izolatları (OP480437-39) ile GenBank’ta kayıtlı Eustrongylides excisus izolatları (MK007967, MT415236, MK545494) arasında tam bir uyum görülmüştür (%0,0). Bu Eustrongylides excisus izolatları Maximum likelihood filogram analizinde aynı kümede toplanmıştır. İzolatların internal transkripsiyonlu ayırıcı (ITS) bölgesi gen sekansı sonuçları, Eustrongylides excisus'un anatomik ve morfolojik özelliklerine göre tanımlanan taksonomik konumunu doğrulamıştır. Ayrıca, Eğirdir Gölü’ndeki Eustrongylides excisus türüne ait 3 örnek üzerinden 18S ve 28SrDNA gen dizileri ilk defa bu çalışmada tanımlanmış ve bu türe ait genetik özelliklerin belirlenmesi çalışmalarına katkı yapılmıştır.

Anahtar Kelimeler

• Eustrongylides excisus
• DNA sekanslama
• Sander lucioperca

Destekleyen Kurum

Afyon Kocatepe University Scientific Research Projects Unit

Proje Numarası

21.Fen.Bil.25

Teşekkür

This study was supported by Afyon Kocatepe University Scientific Research Projects Unit within the scope of project number 21.Fen.Bil.25.

Kaynakça

1. Abe, N. (2011). Molecular and morphological identification of helminthes found in Japanese smelt, Hypomesus trans-pacificus nipponensis, with notes on new host records of Eustrongylidesignotusand Raphidascaris gigi. Acta Para-sitologica, 56:227–231.
2. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. (1990). Basic local alignment search tool. J Mol Biol, 215: 403–410.
3. Balık, İ., Çubuk, H., Özkök, R., Uysal, R. (2006). Eğirdir Gölü balık faunası ve balıkçılığı: sudak balığının (Sander lucio-perca (Linnaeus, 1758)) aşılandığı 1950’li yıllardan günü-müze değişimler, I. Balıklandırma ve Rezervuar Yönetimi Sempozyumu, 105-118, Antalya.
4. Bjelić-Čabrilo, O., Novakov, N., Ćirković, M., Kostić, D., Popović, E., Aleksić, N., vd. (2013). The frst determina-tion of Eustrongylides excisus Jägerskiöld, 1909, Larvae (Nematoda: Dioctophymatidae) in the pike-perch Sander lucioperca in Vojvodina (Serbia). Helminthologia, 50:291–294.
5. Çağlak, E. and Karslı, B. (2013). Beyşehir gölü sudak (San-der lucioperca Linnaeus, 1758) balıklarının mevsimsel et verimi ve kimyasal kompozisyonu. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 9(1): 1-8.
6. Çelik, M., Diler, A., Küçükgülmez, A. (2005). A comparison of the proximate compositions and fatty acid profiles of zan-der (Sander lucioperca) from two different regions and climatic conditions. Food Chemistry, 92(4): 637-641.
7. Çolak, S.Ö. (2013). The helminth community of the sand smelt (Atherina boyeri Risso, 1810) from Lake Iznik, Tur-key. J Helminthol, 87: 129-34.
8. Eiras, J.C., Pavanelli, G.C., Takemoto, R.M., Nawa, Y. (2018). An overview of fish-borne nematodiases among returned travelers for recent 25 years–unexpected disea-ses sometimes far away from the origin. Korean J Parasi-tology, 56: 215.

9. Guardone, L., Ricci, E., Susini, F., Polsinelli, E., Guglielmone, G., Armani, A. (2021). First detection of E.excisus (Ne-matoda: Dioctophymatidae) in big-scale sand smelt (At-herina boyeri) from the lake Massaciuccoli (Northwest Tuscany, Italy). Parasitology Research, 109: 769–780.
10. Ibiwoye, T.T.I., Ogunsusi, R.A., Balogun, A.M., Agbontale, J.J. (2005). Contributions of haematological factors to the estimations of Eustrongylides africanus larvae densities in Clarias gariepinus and Clarias anguilaris from Bida flood plain of Nigeria. Sokoto Journal of Veterinary Sciences, 6: 145-149.
11. Innal, D., Yıldırım, M., Stavrescu-Bedivan, M.M., Güçlü, S.S., Ünal, M.C., Doğangil, B. (2019). Occurrence and infec-tion dynamics of Salsuginus sp. (Monogenea, Ancyro-cephalidae) and Eustrongylides excisus (Nematoda, Di-octophymatidae) in four endemic Aphanius (Cyprinodon-tidae) species: new host records for Turkish fauna. Acta Biologica Turcica, 32(2): 103-109.
12. İlhan, A. and Sarı, H.M. (2013). Fish fauna and fisheries acti-vities in Lake Marmara. Ege Journal of Fisheries and Aquatic Sciences, 30(4): 187–191.
13. Kaptan, H. and Tekin-Özan, S. (2014). Eğirdir Gölü’nün (Is-parta) suyunda, sedimentinde ve gölde yaşayan sazan’ın (Cyprinus carpio L., 1758) bazı doku ve organlarındaki ağır metal düzeylerinin belirlenmesi. SDU Journal of Sci-ence, (E-Journal) 9(2): 44-60.
14. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organi-zation and analysis of sequence data. Bioinformatics, 28: 1647–1649.
15. Koehler, A.V.A., Hoberg, E.P., Torres-Pérez, F., Cook, J.A. (2009). A molecular view of the superfamily Dioc-tophymatoidea (Nematoda). Comparative Parasitology, 76(1): 100–104.
16. Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis ac-ross computing platforms. Molecular Biology and Evolu-tion, 35: 1547-1549.
17. Maci, S. and Basset, A. (2010). Spatio-temporal patterns of abundance, size structure and body condition of Atherina boyeri (Pisces: Atherinidae) in a small non-tidal Mediter-ranean lagoon. Estuarine, Coastal and Shelf Science, 87: 125–134.
18. Mazzone, A., Caffara, M., Gustinelli, A., Agnetti, F., Sgariglia, E., Lo Vaglio, G. (2019). Morphological and molecular characterization of larval and adult stages of Eustrongyli-des excisus (Nematoda: Dioctophymatoidea) with histo-pathological observations. J Parasitology, 105(6): 882–889.
19. Measures, N. (1988). Revision of the genus Eustrongylides Jagerskiold, 1909 (Nematoda: Dioctophymatoidea) of piscivorous birds. Canadian J. Zoology, 66: 885-895.
20. Metin, S., Didinen, B.I., Boyacı, Y.Ö., Kubilay, A., Emre, N., Didinen, H., Emre, Y. (2014). Occurrence of Eustrongyli-des excisus Jägerskiöld 1909 larvae (Nematoda: Dioc-tophymatidae) in pikeperch (Sander lucioperca, L.) in La-ke Eğirdir. Eğirdir Su Ürünleri Fakültesi Dergisi, 10(1): 20-24.
21. Moravec, F. (1994). Parasitic nematodes of freshwater fishes of Europe. Kluwer Academic Publisher, 470p. Moravec, F., Nie, P., Wang, G.T. (2003). Some nematodes of fishes from central China, with the redescription of Procamallanus (Spirocamallanus) fulvidraconis (Camal-lanidae). Folia Parasitologica, 50: 220–230.
22. Ozmen, O., Stavrescu-Bedivan, M.M., Innal, D. (2021). His-topathological aspects caused by nematod parasite Eust-rongylides excisus observed in some fish species of Eğirdir Lake, Turkey. Agro Life Scientific Journal, 10(1): 172-178.
23. Ozturk, O.M., Oğuz, M.C., Altunel, F.N. (2001). Manyas Gölündeki kaya balıkları (Gobius fluviatilis L.)’nın meta-zoon parazitleri üzerine bir araştırma ve Türkiye helminth faunası için iki yeni kayıt. Türkiye Parazitol Dergisi, 25: 88-93.
24. Pekmezci, G.Z. and Bolukbas, C.S. (2021). Morphological and molecular characterization of Eustrongylides excisus larvae (Nematoda: Dioctophymatidae) in Sander lucio-perca (L.) from Northern Turkey. Parasitology Research, 120: 2269–2274.
25. Soylu, E. (2013). Metazoan Parasites of Perch Perca fluviati-lis L. From Lake Sığırcı, Ipsala, Turkey. Pakistan J Zoo-logy, 45(1): 47-52.
26. Spalding, M.G. (1990). Antemortem diagnosis of Eustrongyli-dosis in wading birds (Ciconiiformes). Colonial Water-birds, 13(1): 75–77.
27. Tamura, K., Nei, M., Kumar, S. (2004). Prospects for infer-ring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Scien-ces (USA) 101, 11030-11035.
28. Tanrıkul, T.T., Dinçtürk, E., Dereli, H. (2019). The infection of Eustrongylides spp. in pike perch (Sander lucioperca L., 1758) (Teleostei: Percidae). Anatolian Enviromental and Animal Sciences, 4(2): 122-126.
29. Xiong, F., Wang, G.T., Wu, S.G., Nie, P. (2009). Develop-ment of Eustrongylides ignotus (Nematoda: Dioctophmi-da) in domestic ducks (Anas platyrhynchos domestica (L.). The Journal of Parasitology, 95: 1035–1039.
30. Youssefi, M.R., Tabaripour, R., Hosseini, M. (2020). Molecu-lar characterisation and histopathological study of Eust-rongylides excisus nematode in the northern pike (Esox lucius). Bulgarian Journal of Veterinary Medicine, 26(1): 81-88.