Characterization of Nanocomposites Obtained by Green Synthesis Method Using Althaea officinalis Plant and Evaluation of Their Hemolytic Activities

Öz

In this study, metal oxide nanocomposites were synthesized using egg powder with a non-toxic green synthesis method. Plant extracts were prepared with different solvents (ethanol, methanol, acetone and ultrapure water) from the reddish-pink flower parts of the Althaea officinalis (A. officinalis - marshmallow) plant collected from the Burdur region. Quercetin and gallic acid amounts of these prepared plant extracts were determined by HPLC (High Performance Liquid Chromatography) analysis. According to the HPLC analysis results, nanocomposite structures were synthesized by adding eggshell powder, the main inorganic component of which is CaCO3, which was pulverized with the selected plant extract, to metal solutions. Characterization studies of the obtained nanocomposite structures were carried out by ATR-FTIR (Attenuated Total Reflectance- Fourier Transform Infrared Spectroscopy), XRD (X-ray Diffraction Spectroscopy) and SEM-EDX (Scanning Electron Microscope with Energy-Dispersive X-ray Detector) analyses. As a result of the study, nanocomposites containing MgO and Al2O nanoparticles (NP) were synthesized. It was determined that the synthesized nanocomposites were hemocompatible as a result of hemolysis experiments. Keywords: Eggshell, nanocomposite, green synthesis, metal nanoparticles, Althaea officinalis

Anahtar Kelimeler

• eggshell
• nanocomposite
• green synthesis
• metal nanoparticle
• Althaea officinalis

Yeşil Sentez Yöntemi İle Althaea officinalis Bitkisi Kullanılarak Elde Edilen Nanokompozitlerin Karakterizasyonu ve Hemolitik Aktivitelerinin Değerlendirilmesi

Öz

Bu çalışmada toksik olmayan yeşil sentez yöntemi ile yumurta tozu kullanılarak metal oksit nanokompozitleri sentezlenmiştir. Burdur yöresinden toplanan Althaea officinalis (A. officinalis-hatmi) bitkisinin kırmızımsı-pembe çiçek kısımlarından farklı çözücülerle (etanol, metanol, aseton ve ultra saf su) bitki özütleri hazırlanmıştır. Hazırlanan bu bitki özütlerinin kuersetin ve gallik asit miktarları HPLC (Yüksek Performanslı Sıvı Kromatografisi) analizi ile tespit edilmiştir. HPLC analiz sonuçlarına göre seçilen bitki özütü ile toz hale getirilen temel inorganik bileşeni CaCO3 olan yumurta kabukları tozu metal çözeltilerine eklenerek nanokompozit yapılar sentezlenmiştir. Elde edilen nanokompozit yapıların ATR-FTIR (Zayıflatılmış Toplam Yansıma- Fourier Dönüşümlü Kızılötesi Spektroskopisi), XRD (X-ışını Difraksiyon Spektroskopisi) ve SEM-EDX (Enerji Dağılımlı X-Ray Dedektörlü Taramalı Elektron Mikroskobu) analizleri ile karakterizasyon çalışmaları gerçekleştirilmiştir. Çalışma sonucunda MgO ve Al2O nanopartikülleri (NP) içeren nanokompozitler sentezlenmiştir. Sentezlenen nanokompozitlerin hemoliz deneyleri sonucunda hemouyumlu oldukları tespit edilmiştir. Anahtar kelimeler: Yumurta kabuğu, nanokompozit, yeşil sentez, metal nanopartiküller, Althaea officinalis

Anahtar Kelimeler

• yumurta kabuğu
• nanokompozit
• yeşil sentez
• metal nanopartiküller
• Althaea officinalis

Kaynakça

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