Drosophila melanogaster’de Cetraria islandica (L.) Ach. Likeninin Koruyucu Etkisinin Belirlenmesi

Öz

In this study, the effects of water extract obtained from Cetraria islandica (L.) Ach. Ciwe) on the development of Drosophila melanogaster were investigated. When the different concentrations of Ciwe (0.25; 0.5 and 1.0 mL/100 mL medium) were added to growth medium of adult individuals, no elongation of metamorphosis of F1 progeny was determined. It was observed that the mean number of offspring increased depending on an increase of Ciwe on the application groups. Furthermore, malformed individuals seen in the control group were not observed by adding the different concentrations of Ciwe in the application groups. These differences were statistically signific ant (p< 0.05) according to control group.

Anahtar Kelimeler

• Drosophila melanogaster, Cetraria islandica, Development Stages, Theraupetic Agent, Antioxidant Effect

Determination of Protective Activity of Cetraria islandica (L.) Ach. on Drosophila melanogaster

Öz

In this study, the effects of water extract obtained from Cetraria islandica (L.) Ach. Ciwe) on the development of Drosophila melanogaster were investigated. When the different concentrations of Ciwe (0.25; 0.5 and 1.0 mL/100 mL medium) were added to growth medium of adult individuals, no elongation of metamorphosis of F1 progeny was determined. It was observed that the mean number of offspring increased depending on an increase of Ciwe on the application groups. Furthermore, malformed individuals seen in the control group were not observed by adding the different concentrations of Ciwe in the application groups. These differences were statistically signific ant (p< 0.05) according to control group.

Anahtar Kelimeler

• Drosophila melanogaster, Cetraria islandica, Development Stages, Theraupetic Agent, Antioxidant Effect

Kaynakça

1. Behera, B.C, Adawwadkar, B., & Makhija, U. (2004). Capacity of some Graphidaceous lichens to scavenge superoxide and inhibition of tyrosinase and xanthine oxidase activities. Current Science, 87, 83– 87.
2. Boustie, J., & Grube, M. (2005). Lichens- a promising source of bioactive secondary metabolites. Plant Genetic Resources, 3, 273– 287.
3. Bozcuk, A.N. (1978). The effects of some genotypes on the longevity of adult Drosophila. Experimental Gerontology, 13, 279- 286.
4. Chen, C.W., & Ho, C.T. (1995). Antioxidant properties of polyphenols extracted from green and black teas. Journal of Food Lipids, 2, 35– 46.
5. Duh, P.D., Tu, Y.Y., & Yen, G.C. (1999). Antioxidant activity of aqueous extract of harn jyur (Chyrsanthemum morifolium Ramat). Food Science and Technology Research, 32, 269– 277.
6. Dülger, B., Gücin, F., & Aslan, A. (1998). Antimicrobial activity of Cetraria islandica (L.) Ach. Turkish Journal of Biology, 22, 111– 118.
7. Gülçin, I., Oktay, M., Küfrevioğlu, O.I., & Aslan, A. (2002). Determination of antioxidant activity of lichen Cetraria islandica (L.) Ach. Journal of Ethnopharmacology, 79, 325– 329.
8. Hatano, T., Yasuhara, T., Yoshihara, R., Agata, I., Noro, T., & Okuda, T. (1989). Effects of interaction of tannins with co-existing substances. VII. Inhibitory effects of tannins and related polyphenols on xanthine oxidase. Chemical & Pharmaceutical Bulletin, 37, 2016– 2021.

9. Hidalgo, M.E., Fernande, E., Quilhot, W., & Lissi, E.A. (1993). Photohaemolytic activity of lichen metabolites. Photochemistry and Photobiology B, 21, 37– 40.
10. Hidalgo, M.E., Fernandez, E., Quilhot, W., & Lissi, E. (1994). Antioxidant activity of depsides and depsidones. Phytochemistry, 37, 1585– 1587.
11. Ingolfsdotti, K., Chung, G.A., Skulason, V.G., Gissurarson, S.R., & Vilhelmsdóttir, M. (1998). Antimiyobacteriyal of lichen metabolites in vitro. European Journal of Pharmaceutical Sciences, 6, 141– 144.
12. Jayaprakasha, G.K., & Rao, L.J. (2000). Phenolic constituents fromthe lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activities. Zeitschrift für Naturforschung C, 55c, 1018– 1022.
13. Lemonica, I.P., Damasceno, D.C., & di-Stasi, L.C. (1996). Study of the embryotoxic effects of an extract of rosemary (Rosmarinus officinalis L.). Brazilian Journal of Medical and Biological Research, 29(2), 223- 227.
14. Malencic, D.J., Gasic, O., Popovic, M., & Boza, P. (2000). Screening for antioxidant properties of Salvia reflexa hornem. Phytotherapy Research, 14, 546– 548.
15. Menegazzi, M., Di Paola, R., Mazzon, E., Muià, C., Genovese, T., Crisafulli, C., Suzuki, H., & Cuzzocrea, S. (2006). Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. Free Radical Biology & Medicine, 40(5), 740- 753.
16. Ogmundsdottir, H.M., Zoega, G.M., Gissurarson, S.R., & Ingólfsdóttir, K. (1998). Anti- proliferative effects of lichen-derived inhibitors of 5-lipoxygenase on malignant cell- lines and mitogen- stimulated lymphocytes. The Journal of Pharmacy and Pharmacology, 50, 107– 115.
17. Pengsuparp, T., Cai, L., Constant, H., Fong, H.H.S., Lin, L.Z., Kinghorn, A.D., Pezzuto, J.M., Cordell, G.A., Ingolfsdöttir, K., Wagner, H., & Hughes, S.H. (1995). Mechanistic evaluation of new plant-derived compounds that inhibit HIV-1 reverse transcriptase. Journal of Natural Products, 58, 1024– 1031.
18. Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F. (1999). Free radical scavenging capacity an inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Research International, 32, 407– 412.
19. Schempp, C.M., Kiss, J., Kirkin, V., Averbeck, M., Simon-Haarhaus, B., Kremer, B., Termeer, C.C., Sleeman, J., & Simon, J.C. (2005). Hyperforin acts as an angiogenesis inhibitor. Planta Medica, 71(11), 999- 1004.
20. Shukla, P.K., Khanna, V.K., Ali, M.M., Maurya, R.R., Handa, S.S., & Srimal, R.C. (2002). Protective effect of Acorus calamus against acrylamide induced neurotoxicity. Phytother Research, 16, 256- 260.
21. Srinivasan, D., Nathan, S., Suresh, T., & Lakshmana Perumalsamy, P. (2001). Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. Journal of Ethnopharmacology, 74(3), 217- 220.
22. Stepanenko, L.S., Krivoshchekova, O.E., Dimitrenok, P.S., & Maximov, O.B. (1997). Quinones of Cetraria islandica. Phytochemistry, 46, 565- 568.
23. Stajner, D., Milic, N., Mimica-Dukic, N., Lazıc, B., & Igıc, R. (1998). Antioxidant abilities of cultivated and wild species of garlic. Phytother Research, 12, 513– 514.
24. Tanaka, M., Kuei, C.W., & Nagashima, Y. (1998). Application of antioxidative maillrad reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkai Shi. Bulletin Of The Japanese Society Of Scientific Fisheries, 54, 1409– 1414.
25. Uysal, H., Kara, A.A., Algur, O.F., Dumlupinar, R., & Aydogan, M.N. (2007). Recovering effects of aqueous extracts of some selected medical plants on the teratogenic effects during the development of D. melanogaster. Pakistan Journal of Biological Sciences, 10(10), 1708- 1712.
26. Uysal, H., Altun, D., Aşkın, H., & Aslan, A. (2009). The Effects of Water Extract of Usnea longissima Ach. on Some Development Stages in Drosophila melanogaster. Fresenius Environmental Bulletin, 18(4), 450- 455.