Effects of Entomopathogenic Fungi on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)

Öz

The study investigated the efficacy of five entomopathogenic fungi [Aspergillus sp., Beauveria sp., Fusarium sp., Trichoderma sp. (1) and Trichoderma sp. (2)] isolated from soil samples which were taken in the wheat farming of INPV (National Institute of Plant Protection of Constantine, Constantine-Algeria) on the second-stage larvae of Tuta absoluta (Lepidoptera: Gelechiidae). Spore suspensions of fungi were applied by spraying method after dilution with sterile distilled water containing 0.02% Tween 80, so that 10⁷ spores/ml concentration. Individuals exposed to distilled water containing Tween 80 were used as the control group in order to compare the effect of entomopathogenic fungi. Evaluations of infection development were made at the 1^st, 3^rd, 5^th and 7^th days following applications. According to the results obtained, the highest mortality at the 7th DAA (Day After Application) was found as 75.00% and 73.00% from the individuals exposed to of with Trichoderma sp. (1) and with Trichoderma sp. (2) entomopathogenic fungi, respectively. These entomopathogenic fungi followed %62.00, %45.00 and %41.00 mortality of Aspergillus sp., Fusarium sp. and Beauveria sp., respectively.

Anahtar Kelimeler

• Aspergillus sp.,Beauveria sp.,Fusarium sp.,Tuta absoluta,Trichoderma sp.

Entomopatojen Fungusların Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)’ ya Etkileri

Öz

Bu çalışmada, Ulusal Bitki Koruma Enstitüsü (INPV-National Institute of Plant Protection of Constantine, Constantine-Algeria) çevresinden alınan toprak örneklerinden izole edilen 5 farklı entomopatojen fungus [Aspergillus sp., Beauveria sp., Fusarium sp., Trichoderma sp. (1) ve Trichoderma sp. (2)]’un Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) 2. dönem larvaları üzerine etkinliği araştırılmıştır. Fungusların spor süspansiyonları 10⁷ spor/ml olacak şekilde %0,02 oranında Tween 80 içeren steril saf su ile seyreltildikten sonra püskürtme yöntemiyle uygulanırken, Tween 80 içeren steril saf su uygulanmış bireyler ise kontrol grubu olarak kabul edilmiştir. Enfeksiyon gelişimi değerlendirmeleri, uygulamalar yapıldıktan 1, 3, 5 ve 7 gün sonra canlı birey sayıları üzerinden yapılmıştır. Elde edilen sonuçlara göre, uygulamadan sonraki 7. günde, kontrol grubuna göre en yüksek yüzde ölüm oranı %75,00 ile Trichoderma sp. (1) ve %73,00 ölüm oranı ile Trichoderma sp. (2) entomopatojen funguslarında elde edilmiştir. Bu fungusları sırasıyla %62,00, %45,00 ve %41,00 ölüm oranı ile - Aspergillus sp., Fusarium sp. ve Beauveria sp. fungusları takip etmiştir.

Anahtar Kelimeler

• Trichoderma sp.,Aspergillus sp.,Fusarium sp.,Beauveria sp.,Tuta absoluta,Trichoderma sp.

Kaynakça

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