Determination of Anti-MRSA and Antimicrobial Activity of Ramaria aurea Extracts

Öz

Infectious diseases have been one of the leading causes of death worldwide for years. Especially multidrug-resistant Methicillin-Resistant Staphylococcus aureus (MRSA) strains are among the most important threats to public health worldwide. Basidiomycetes group fungi are known as a promising source of antibacterial compounds. This study aimed to determine the antimicrobial activity of methanol and ethanol extracts of Ramaria aurea (Schaeff.) Quél. against pathogenic standard microorganisms and anti-MRSA activity against MRSA strains isolated from clinical samples. Broth microdilution method was used to determine the antimicrobial activity. Concentrations between 12.5-0.006 mg/ml of the extracts were applied to the strains used. While methanol and ethanol extracts were found to have antibacterial activity against standard bacterial strains at doses ranging from 0.19 to 6.25 mg/ml, the most effective strain of both extracts was Bacillus cereus at a dose of 0.19 mg/ml. In addition, no antifungal effect was determined for both methanol and ethanol extracts. While methanol extract was found to have anti-MRSA activity against all studied MRSA strains at doses ranging from 0.04 to 6.25 mg/ml, ethanol extract was found to have anti-MRSA activity against all these strains at doses ranging from 0.19 to 3.12 mg/ml. As a result, it was determined that the studied Ramaria aurea extracts had antibacterial and anti-MRSA activity, and it can be said that this species may be a natural source of antimicrobial agents and may be used in the pharmaceutical industry.

Anahtar Kelimeler

• Antifungal
• Antimicrobial
• Anti-MRSA activity
• Basidiomycota
• Methicillin Resistant Staphylococcus aureus

Ramaria aurea Ekstraktlarının Anti-MRSA ve Antimikrobiyal Aktivitesinin Belirlenmesi

Öz

Bulaşıcı hastalıklar yıllardan beri dünya çapında ölümlerin önde gelen nedenlerinden biridir. Özellikle çoklu ilaç direncine sahip Metisiline Dirençli Staphylococcus aureus (MRSA) suşları, dünya çapında halk sağlığını tehdit eden en önemli unsurların başında gelir. Basidiomycetes grubu mantarlar umut verici bir antibakteriyel bileşik kaynağı olarak bilinmektedirler. Bu çalışmada Ramaria aurea (Schaeff.) Quél.’nın metanol ve etanol ekstraktlarının patojen standart mikroorganizmalara karşı antimikrobiyal ve klinik örneklerden izole edilen MRSA suşlarına karşı anti-MRSA aktivitesinin belirlenmesi amaçlanmıştır. Antimikrobiyal aktiviteyi belirlemek için sıvı mikrodilüsyon metodu kullanılmıştır. Kullanılan suşlara ekstraktların 12.5-0.006 mg/ml arasındaki konsantrasyonları uygulanmıştır. Metanol ve etanol ekstraktlarının, standart bakteri suşlarına karşı 0.19-6.25 mg/ml aralığındaki dozlarda antibakteriyal aktiviteye sahip olduğu tespit edilirken, her iki ekstraktın da en etkili olduğu suşun 0.19 mg/ml dozla Bacillus cereus suşu olduğu görülmüştür. Ayrıca metanol ve etanol ekstraklarının her ikisinin de antifungal bir etkisi belirlenememiştir. Metanol ekstraktının çalışılan tüm MRSA suşlarına karşı 0.04-6.25 mg/ml aralığındaki dozlarda anti-MRSA aktivitesi gösterdiği belirlenirken, etanol ekstraktının bu suşların tamamına karşı 0.19-3.12 mg/ml aralığındaki dozlarda anti-MRSA aktivitesine sahip olduğu tespit edilmiştir. Sonuç olarak çalışılan Ramaria aurea ekstraklarının antibakteriyal ve anti-MRSA etkiye sahip olduğu belirlenmiş olup bu türün antimikrobiyal ajanların doğal bir kaynağı olabileceği ve farmakoloji endüstrisinde kullanılabileceği söylenebilir.

Anahtar Kelimeler

• Antifungal
• Antimikrobiyal
• Anti-MRSA aktivite
• Basidiomycota
• Metisilin dirençli Staphylococcus aureus

Kaynakça

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