In vitro Antimicrobial Activity of Desarmillaria tabescens

Öz

Mushrooms are known to be nutritive and medicinal food stuff, which are good sources of some vitamins and essential minerals. They also contain some therapeutic agents, thus they have been used against several health problems for hundreds of years. The aim of this study is to determine the in vitro antimicrobial activity of Desarmillaria tabescens (Scop.) R.A. Koch & Aime 2017.

D. tabescens samples were air dried and extracted by using ethanol. Antimicrobial activity of D. tabescens ethanol extracts were investigated against several Gram positive and Gram negative bacteria strains, fungal strains, which are either standard or isolated from food and some multi drug resistant (MDR) clinical isolate bacteria namely, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) and Streptococcus pneumoniae (MDR) by using the disk diffusion method.

As a result, it was observed that ethanol extracts of D. tabescens has low to medium antimicrobial activity against several Gram positive and Gram negative microorganisms tested. The antimicrobial activity of D. tabescens especially observed against K. pneumoniae (MDR) and S. pneumoniae (MDR) is found to be remarkable.

Anahtar Kelimeler

• Desarmillaria tabescens,antimicrobial activity,disk diffusion,multi drug resistant bacteria,MDR

Desarmillaria tabescens’in in vitro Antimikrobiyal Aktivitesi

Öz

Mantarların, bazı vitaminlerin ve temel minerallerin kaynağı olan besleyici ve tıbbi gıda maddeleri olduğu bilinmektedir. Ayrıca bazı terapötik maddeler içerirler, bu yüzden yüzlerce yıldır çeşitli sağlık problemlerine karşı kullanılmıştır. Bu çalışmanın amacı Desarmillaria tabescens (Scop.) R.A. Koch & Aime 2017’nin in vitro antimikrobiyal aktivitesini belirlemektir.

D. tabescens örnekleri kurutulmuş ve etanol kullanılarak ekstre edilmiştir. D. tabescens etanol ekstraktlarının antimikrobiyal aktivitesi, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria innocua, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 5071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis DSMZ 20044, Staphylococcus aureus (MDR), Escherichia coli (MDR), Klebsiella pneumoniae (MDR), Acinetobacter baumannii (MDR) ve Streptococcus pneumoniae (MDR) gibi Gram pozitif ve Gram negatif standart, gıdadan izole edilmiş bakteri suşları, mantar suşları ve klinik izole bazı çoklu ilaca dirençli (MDR) klinik izolat bakteriler kullanılarak disk difüzyon yöntemini ile araştırılmıştır.

Sonuç olarak, D. tabescens'in etanol ekstraktlarının, test edilen bazı Gram pozitif ve Gram negatif mikroorganizmaya karşı düşük ila orta antimikrobiyal aktiviteye sahip olduğu gözlenmiştir. D. tabescens'in özellikle K. pneumoniae (MDR) ve S. pneumoniae'ye (MDR) karşı gözlenen antimikrobiyal aktivitesinin dikkat çekici olduğu bulunmuştur.

Anahtar Kelimeler

• Desarmillaria tabescens,antimikrobiyal aktivite,disk difüzyon,çoklu ilaca dirençli bakteriler,MDR

Kaynakça

1. Agrahar-Murugkar, D., and Subbulakshmi, G. (2005). Nutritional value of edible wild mushrooms collected from the Khasi hills of Meghalaya. Food Chemistry, 89(4), 599-603.
2. Altuner, E. M., Akata, I., and Canlı, K. (2012). In vitro Antimicrobial Activity Screening of Bovista nigrescens Pers. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 12(1), 90-96.
3. Altuner, E. M., and Canli, K. (2012). In vitro antimicrobial screening of Hypnum andoi AJE Sm. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 12(1), 97-101.
4. Altuner, E. M., Canli, K., and Akata, I. (2014). Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme. Journal of Pure and Applied Microbiology, 8(1), 539-545.
5. Andrews, J. M. (2007). BSAC standardized disc susceptibility testing method (version 6). Journal of antimicrobial chemotherapy, 60(1), 20-41.
6. Bala, N., Aitken, E. A., Fechner, N., Cusack, A., and Steadman, K. J. (2011). Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well plate assay. Pharmaceutical biology, 49(5), 492-500.
7. Bonatti, M., Karnopp, P., Soares, H. M., and Furlan, S. A. (2004). Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food chemistry, 88(3), 425-428.
8. Canli, K., Akata, I., and Altuner, E. M. (2016b). In vitro antimicrobial activity screening of Xylaria hypoxylon. African Journal of Traditional, Complementary and Alternative medicines, 13(4), 42-46.

9. Canli, K., Altuner, E. M., Akata, I., Turkmen, Y., and Uzek, U. (2016a). In vitro antimicrobial screening of Lycoperdon lividum and determination of the ethanol extract composition by gas chromatography/mass spectrometry. Bangladesh Journal of Pharmacology, 11(2), 389-394.
10. Canli, K., Yetgin, A., Akata, I., and Altuner, E. M. (2016c). In vitro antimicrobial screening of Aquilaria agallocha roots. African Journal of Traditional, Complementary and Alternative Medicines, 13(5), 178-181.
11. Cardwell, G., Bornman, J., James, A., and Black, L. (2018). A review of mushrooms as a potential source of dietary vitamin D. Nutrients, 10(10), 1498.
12. Cheung, L. M., and Cheung, P. C. (2005). Mushroom extracts with antioxidant activity against lipid peroxidation. Food Chemistry, 89(3), 403-409.
13. Clardy, J., and Walsh, C. (2004). Lessons from natural molecules. Nature, 432(7019), 829-837.
14. Core R Team, R: A language and environment for statistical computing. R Foundation for Statistical Computing, https://www.R-project.org/ (last accession: 15.08.2019).
15. Donnelly, D. M., Konishi, T., Dunne, O., and Cremin, P. (1997). Sesquiterpene aryl esters from Armillaria tabescens. Phytochemistry, 44(8), 1473-1478.
16. Dülger, B., Fedai, Ş. E. N., and Gücin, F. (1999). Antimicrobial Activity of The Macrofungi Russula delica Fr. Turkish Journal of Biology, 23(1), 127-134.
17. Dulger, B., Suerdem, T. B., Yesilyurt, D., Hacioglu, N., and Camdeviren, A. (2005). Evaluation of antimicrobial activity of the macrofungus Phellinus torulosus. Journal of Biological Sciences, 5(4), 436-439.
18. Dundar, A., Okumus, V., Ozdemir, S., Celik, K. S., Boga, M., Ozcagli, E., Ozhan, G., and Yildiz, A. (2015). Antioxidant, antimicrobial, cytotoxic and anticholinesterase activities of seven mushroom species with their phenolic acid composition. Journal of Horticulture, 2(4), 1000161.
19. Falandysz, J. (2008). Selenium in edible mushrooms. Journal of Environmental Science and Health Part C, 26(3), 256-299.
20. Gunde-Cimerman, N. (1999). Medicinal value of the genus Pleurotus (Fr.) P. Karst.(agaricales sl, Basidiomycetes). International Journal of Medicinal Mushrooms, 1(1), 69-80.
21. Hammer, K. A., Carson, C. F., and Riley, T. V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology, 86(6), 985-990.
22. Herath, H. B., Jacob, M., Wilson, A. D., Abbas, H. K., and Nanayakkara, N. D. (2013). New secondary metabolites from bioactive extracts of the fungus Armillaria tabescens. Natural product research, 27(17), 1562-1568.
23. Imtiaj, A., and Lee, T. S. (2007). Screening of antibacterial and antifungal activities from Korean wild mushrooms. World journal of agricultural sciences, 3(3), 316-321.
24. Mahasneh, A. M., and El-Oqlah, A. A. (1999). Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan. Journal of Ethnopharmacology, 64(3), 271-276.
25. Onbasili, D., Altuner, E. M., and Çelik, G. Y. (2011). Mnium marginatum özütlerinin antimikrobiyal aktivitesi. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 11(2), 205-208.
26. Ooi, V.E.C. (2010) Medicinally important fungi. In: Van Griensven editor. Science and cultivation of edible fungi, Rotterdam: Balkema.
27. Paudel, B., Bhattarai, H. D., Lee, J. S., Hong, S. G., Shin, H. W., and Yim, J. H. (2008). Antibacterial potential of Antarctic lichens against human pathogenic Gram‐positive bacteria. Phytotherapy research, 22(9), 1269-1271.
28. Silici, S., and Koc, A. N. (2006). Comparative study of in vitro methods to analyse the antifungal activity of propolis against yeasts isolated from patients with superficial mycoses. Letters in applied microbiology, 43(3), 318-324.
29. Wasser, S. P., and Weis, A. L. (1999a). Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives. International Journal of medicinal mushrooms, 1(1), 31-62.
30. Wasser, S. P., and Weis, A. L. (1999b). Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: a modern perspective. Critical Reviews™ in Immunology, 19(1), 65-96.
31. Watanabe, F., Yabuta, Y., Bito, T. and Teng, F. (2014). Vitamin B12-containing plant food sources for vegetarians. Nutrients, 6(5), 1861-1873.
32. Webster, D., Taschereau, P., Belland, R. J., Sand, C., and Rennie, R. P. (2008). Antifungal activity of medicinal plant extracts; preliminary screening studies. Journal of Ethnopharmacology, 115(1), 140-146.
33. WHO, Antibiotic resistance, https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance (last accession: 15.08.2019).