Ramalina calicaris (L.) Fr. Liken Türünün Antibakteriyel ve Antioksidan Aktivitesi

Yıl 2018, Cilt: 30 Sayı: 3, 269 - 275, 30.09.2018

Damla Güneş Yücel , Gülşah Özyiğitoğlu

https://doi.org/10.7240/marufbd.402055

Cited By: 1

Öz

Bu
çalışmada Ramalina calicaris (L.) Fr. liken türünün antibakteriyel ve
antioksidan aktivitesinin araştırılması amaçlanmıştır. Bolu’da iki farklı
lokasyondan (Mudurnu-Aladağlar) toplanmış olan Ramalina calicaris türüne ait liken örnekleri; ayrıştırma ve teşhis
yapıldıktan sonra yıkanmış, kurutulmuş ve aseton çözücüsü kullanılarak özütleri
çıkarılmıştır. Liken aseton özütlerinin antibakteriyel aktiviteleri iki Gram
negatif (CECT 4122 Pseudomonas aeruginosa,
ATCC 25922 Escherichia coli) ve iki
Gram pozitif (ATCC 29212 Enterococcus
faecalis, ATCC 25923 Staphylococcus
aureus) bakteri üzerinde disk difüzyon yöntemiyle araştırılmıştır. Araştırma
sonucunda aynı ilde iki farklı lokasyondan toplanmış olan liken örneklerine ait
özütlerin ikisinin de sadece Gram pozitif olan E. faecalis ve S. aureus
üzerinde antibakteriyel aktiviteye sahip olduğu, fakat antibakteriyel
aktiviteleri arasında çok büyük farklılıklar olmadığı belirlenmiştir. Ayrıca,
antibakteriyel etkinliği nispeten daha yüksek bulunan Bolu-Aladağlar
lokasyonundan toplanmış olan R.calicaris
örneklerinin CUPRAC metodu kullanılarak antioksidan aktivitesi de araştırılmıştır.
R. calicaris aseton özütünün belirli
bir düzeyde kromojenik oksidan indirgeme kapasitesine sahip olduğu, dolayısıyla
antioksidan aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Liken, Ramalina calicaris, antibakteriyel aktivite, antioksidan aktivite

Antibacterial and Antioxidant Activities of the Lichen Species Ramalina calicaris (L.) Fr.

Öz

In
this study, the antibacterial and antioxidant activities of the lichen species Ramalina calicaris (L.) Fr. were
investigated. Lichen samples belonging to Ramalina
calicaris were collected from two different locations (Mudurnu-Aladağlar)
in Bolu. After the separation and the identification steps, the samples were
washed, dried and then extracted using an acetone solvent. Antibacterial
activities of the acetone extracts were investigated by disk diffusion method
on two Gram negative (CECT 4122 Pseudomonas
aeruginosa, ATCC 25922 Escherichia
coli) and two Gram positive (ATCC 29212 Enterococcus
faecalis, ATCC 25923 Staphylococcus
aureus) bacteria. As a result of the study, the both extracts of lichen
samples collected from two different locations in Bolu had antibacterial
activity on only Gram positive E.
faecalis and S. aureus. However,
they did not show a great difference between their antibacterial activities. Antioxidant activity studies were also carried out with R. calicaris samples collected from
Bolu-Aladağlar location, which indicated relatively higher antibacterial
activity by using CUPRAC method. It has been found that R.
calicaris acetone extract has antioxidant activity as it has a certain
level of chromogenic oxidant reduction capacity.

Anahtar Kelimeler

Lichen, Ramalina calicaris, antibacterial activity, antioxidant activity

Kaynakça

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